Plasma glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), non‐esterified free acid (NEFA), TG, total cholesterol, low‐density lipoprotein (LDL), high‐density lipoprotein (HDL) and glucose levels (Glu) were measured with L‐type Wako GOT‐J2, L‐type Wako GPT‐J2, NEFA‐HA Test Wako, L‐type Wako TG‐H, L‐type CHO H, L‐Type LDL‐C, L‐Type HDL‐C and L‐Type Glucose 2 (Wako Pure Chemical Industries, Osaka, Japan), respectively. 3‐Hydroxybutyrate was determined by Ketone test liquid (Sanwa Kagaku Kenkyusho, Nagoya, Japan) using auto analyser (7170, Hitachi Ltd., Tokyo, Japan). Plasma GIP, insulin, and adiponectin levels were measured using enzyme‐linked immunosorbent assay kits (LINCO RESEARCH, INC., Missouri, U.S.A., Shibayagi, Gumma, Japan, and Otsuka Pharmaceutical Ltd., Tokushima, Japan, respectively).
Auto analyser 7170
Manufactured by Hitachi
Sourced in Japan
The Hitachi Auto Analyser (7170) is a laboratory instrument designed for automated chemical analysis. It is capable of performing various types of analysis, such as colorimetric, enzymatic, and immunochemical tests, on a wide range of sample types. The instrument is equipped with a high-speed, precise liquid handling system and can handle multiple samples simultaneously, enabling efficient and reliable analysis in a laboratory setting.
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Lab products found in correlation
Enzyme linked immunosorbent assay kit, by Linco Research (1 mentions)
L type ldl c, by Fujifilm (1 mentions)
L type hdl c, by Fujifilm (1 mentions)
L type wako tg h, by Fujifilm (1 mentions)
Human insulin, by Santa Cruz Biotechnology (1 mentions)
Microplate reader, by Tecan (1 mentions)
Anti insulin, by Abcam (1 mentions)
Free fatty acid assay kit, by Abcam (1 mentions)
Accu check active, by Roche (1 mentions)
Anti fabp4, by Abcam (1 mentions)
2 protocols using auto analyser 7170
1
Metabolic Biomarker Quantification Protocol
2
Metabolic Biomarker Profiling in Mice
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Serum concentrations of alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatinine (Cr), urea nitrogen, triglycerides (TG), total cholesterol (TC), low‐density lipoprotein cholesterol (LDL‐C) and high‐density lipoprotein cholesterol (HDL‐C) were determined using an automatic biochemical analyser (Hitachi Auto Analyser 7170, Japan).
Serum concentrations of insulin and FABP4 were determined by sandwich ELISA. The primary antibodies were as follows: anti‐insulin and anti‐FABP4 (Abcam, Cambridge, UK). The absorbance was determined at 450 nm on a microplate reader (Tecan Group AG, Männedorf, Switzerland). The level of nonesterified fatty acids (NEFAs) was detected using the Free Fatty Acid Assay Kit (Abcam, Cambridge, UK) according to the manufacturer's instructions.
Mouse blood was dropped onto a glucose test strip (Accu‐Check Active, Roche) and measured by a glucometer (Accu‐Check Active, Roche). For the glucose tolerance test (IP‐GTT), mice were given an intraperitoneal injection (ip) of 100 mg/mL D‐glucose (2 g/kg body weight) after overnight fasting, and blood glucose concentrations were monitored. For the insulin tolerance test (IP‐ITT), mice were fasted for 4 hours before ip administration of human insulin (0.75 U/kg body weight, Santa Cruz Biotechnology, Dallas, TX), and blood glucose concentrations were monitored.
Serum concentrations of insulin and FABP4 were determined by sandwich ELISA. The primary antibodies were as follows: anti‐insulin and anti‐FABP4 (Abcam, Cambridge, UK). The absorbance was determined at 450 nm on a microplate reader (Tecan Group AG, Männedorf, Switzerland). The level of nonesterified fatty acids (NEFAs) was detected using the Free Fatty Acid Assay Kit (Abcam, Cambridge, UK) according to the manufacturer's instructions.
Mouse blood was dropped onto a glucose test strip (Accu‐Check Active, Roche) and measured by a glucometer (Accu‐Check Active, Roche). For the glucose tolerance test (IP‐GTT), mice were given an intraperitoneal injection (ip) of 100 mg/mL D‐glucose (2 g/kg body weight) after overnight fasting, and blood glucose concentrations were monitored. For the insulin tolerance test (IP‐ITT), mice were fasted for 4 hours before ip administration of human insulin (0.75 U/kg body weight, Santa Cruz Biotechnology, Dallas, TX), and blood glucose concentrations were monitored.
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