The morphology of the electrospun fiber mats was characterized using a scanning electron microscope [SEM, ETH: 2 kV, Everhart-Thornley detector (SE2), AURIGA base 55, Carl Zeiss]. The samples were coated with a thin layer of gold (Q150T Turbo-Pumped Sputter Coater/Carbon Coater, Quorum Technologies) before SEM observations. The average diameter and diameter distribution of electrospun fibers was estimated from the SEM micrographs via Image J analysis software (NIH, USA). The average fiber diameter was measured randomly at 50 different points for each sample.
Q150t turbo pumped sputter coater carbon coater
Manufactured by Quorum Technologies
Sourced in United Kingdom
The Q150T Turbo-Pumped Sputter Coater/Carbon Coater is a versatile laboratory equipment used for depositing thin films of materials onto various substrates. It employs a vacuum-based sputtering process to coat samples with a controlled thickness of the desired material, such as metals, alloys, or carbon. The Q150T features a turbo-molecular pump for efficient evacuation of the chamber, ensuring a high-quality coating environment.
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5 protocols using q150t turbo pumped sputter coater carbon coater
1
Characterization of Electrospun Fiber Mats
2
High-Resolution SEM Analysis of Powder Samples
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Scanning electron microscope (SEM) analysis was performed with a high-resolution field emission SEM (HR-FESEM; model Auriga 405; Carl Zeiss Microscopy GmbH, Jena, Germany) equipped with an energy dispersive spectrometer for X-ray microanalysis (XEDS, model Quantax; Bruker, Berlin, Germany). Before analysis, a small portion of the powder sample was fixed to specific support and coated with a thin layer of C by a sputtering machine (Q150T Turbo-Pumped Sputter Coater/Carbon Coater; Quorum Technologies Ltd., East Sussex, United Kingdom) to make its surface conductive. HR-FESEM XEDS acquisitions were performed under a high vacuum (10−6 hPa) at 20 keV accelerating voltage. Micrographs were acquired by secondary electron detector (SED) at a working distance (WD), magnification, spot size, and tilt angle conditions properly adjusted to optimize image resolution. The microanalysis was performed at WD and magnification ranging from 3.0 to 7.8 mm and 100× to 45,000×, respectively.
3
SEM Imaging of Surface Samples
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Surfaces were washed in sterile water and left to air dry. Surfaces were then
sputter coated with 7 nm platinum using Q150T turbo-pumped sputter coater/carbon
coater (Quorum Technologies Ltd, East Sussex, UK). In order to ensure charge
distribution, Electrodag 1415 (AGG3648, Agar scientific, Essex, UK) was painted
from the edge of surfaces to metal mounting stub. Samples were viewed using
Quanta FEG 250 scanning electron microscope (SEM; FEI™, Eindhoven, The
Netherlands). The SEM was controlled by xT microscope control software, which
also allowed for image capturing.
sputter coated with 7 nm platinum using Q150T turbo-pumped sputter coater/carbon
coater (Quorum Technologies Ltd, East Sussex, UK). In order to ensure charge
distribution, Electrodag 1415 (AGG3648, Agar scientific, Essex, UK) was painted
from the edge of surfaces to metal mounting stub. Samples were viewed using
Quanta FEG 250 scanning electron microscope (SEM; FEI™, Eindhoven, The
Netherlands). The SEM was controlled by xT microscope control software, which
also allowed for image capturing.
4
Scanning Electron Microscopy of Alginate-Gelatin Microcapsules
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For scanning electron microscopy (Carl Zeiss Scanning Electron Microscope; 2 kV, Everhart-Thornley, detector SE2, AURIGA base 55), microcapsules were fixed following reported protocols [18] . Briefly, alginate-gelatin microcapsules were washed in 0.1 M HEPES buffer, and resuspended in 4.0% w/v Paraformaldehyde, keeping them in this solution for three days (4°C). After that, capsules were dehydrated, using consecutive immersion steps in 30%, 50%, 70%, 80%, 90%, 95% and 100% v/v ethanol (Roth, Germany) for 24 -48 hours. Capsules then were critical point dried with a Leica EM CPD300 device, and coated with gold particles (Q150T Turbo-Pumped Sputter Coater/Carbon Coater, Quorum Technologies).
5
Graphite Metallization and SEM Analysis
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Semi-thin sections were dehydrated and metalliszed with graphite by "Q150T Turbo-Pumped Sputter Coater/Carbon Coater" (Quorum Technologies Ltd., Laughton, United Kingdom); EDX microanalysis was performed in a Zeiss SEM LEO 1525 (LEO Electron Microscopy Inc., One Zeiss Drive, Thornwood, NY).
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