Anti uvrag

Manufactured by Cell Signaling Technology

Sourced in United States

Anti-UVRAG is a primary antibody that specifically recognizes the UVRAG protein. UVRAG is a protein involved in the regulation of autophagy, a cellular process that degrades and recycles damaged or unnecessary cellular components. The Anti-UVRAG antibody can be used in various applications, such as Western blotting, immunoprecipitation, and immunohistochemistry, to detect and study the UVRAG protein.

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Lab products found in correlation

Anti vps34, by Cell Signaling Technology (4 mentions) Anti atg14, by Cell Signaling Technology (3 mentions) Mini protean tgx precast gel, by Bio-Rad (2 mentions) Anti beclin 1, by Santa Cruz Biotechnology (2 mentions) Anti ampkα, by Cell Signaling Technology (2 mentions) Anti p raptor ser792, by Cell Signaling Technology (2 mentions) Anti acc, by Merck Group (2 mentions) Anti flag m2 hrp, by Merck Group (2 mentions) Anti p ampkα thr172, by Cell Signaling Technology (2 mentions) Anti β actin hrp, by Santa Cruz Biotechnology (2 mentions) Anti myc hrp sc40 hrp, by Santa Cruz Biotechnology (2 mentions) Anti tbc1d1, by Cell Signaling Technology (2 mentions) Anti p acc ser79, by Merck Group (2 mentions) Anti p tbc1d1 ser237, by Merck Group (2 mentions) Anti ha high affinity hrp, by Roche (2 mentions) Anti raptor, by Cell Signaling Technology (2 mentions) Anti atg14, by MBL Life Science (2 mentions) Anti beclin 1, by Cell Signaling Technology (2 mentions) Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions) Anti sqstm1 p62, by Abcam (1 mentions)

Close spelling variants of this product

UVRAG (6 citations)

6 protocols using anti uvrag

Antibodies for Western Blot Analysis

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All primary antibodies used for western blots were purchased from commercial sources. Antibodies from Santa Cruz include: anti-beclin 1 (sc11427), anti-myc HRP (sc40 HRP) and anti-β-actin HRP (sc47778 HRP). Antibodies from Cell Signaling Technology include: anti-UVRAG (#11315), anti-Vps34 (#4263), anti-Atg14 (#96752, to detect mouse Atg14), anti-p-AMPKα(Thr172) (#2535), anti-AMPKα (#2532), anti-p-Raptor(Ser792) (#2083), anti-Raptor (#2280), anti-TBC1D1 (#66433) and anti-HA (#3724, to detect Tlr9-HA in tissue lysates of Tlr9-HA KI mice). Other primary antibodies include: anti-p-ACC(Ser79) (#07–303, Millipore), anti-ACC (#04–322, Millipore), anti-p-TBC1D1(Ser237) (#07–2268, Millipore), anti-Atg14 (M184–3, MBL International, to detect human ATG14), anti-HA high affinity-HRP (#12013819001, Roche, to detect overexpressed TLR9-HA in cells and Tlr9-HA after immunoprecipitation from muscle lysates of the Tlr9-HA KI mice), anti-Flag M2-HRP (A8592, Sigma), and anti-GLUT4 (GT41-A, Alpha Diagnonstic International). To detect beclin 1 in Tlr9-HA immunoprecipitates from muscle lysates by western blot analysis, 10% MINI-PROTEAN TGX Precast gels (Bio-Rad) were used. For detection of all other proteins, 4–20% Precast gels were used.

Liu Y., Nguyen P.T., Wang X., Zhao Y., Meacham C.E., Zou Z., Bordieanu B., Johanns M., Vertommen D., Wijshake T., May H., Xiao G., Shoji-Kawata S., Rider M.H., Morrison S.J., Mishra P, & Levine B. (2020). Tlr9 and Beclin 1 Cross-Talk Regulates Muscle AMPK Activation in Exercise. Nature, 578(7796), 605-609.

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Antibodies for Western Blot Analysis

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Western Blot Analysis of Apoptosis and Autophagy

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Total proteins were extracted from the collected cells. Equal amounts of protein were separated via SDS-PAGE and then transferred onto polyvinylidene fluoride (PVDF) membranes (Merck Millipore). The membranes were subsequently blocked for 2 h in Tris-buffered saline containing 0.1% Tween and 5% nonfat dry milk and then incubated with primary antibodies overnight at 4 °C. After incubation with horseradish peroxidase-conjugated secondary antibodies (1:5000; Multi Sciences Biotech, Hangzhou, China), the PVDF membranes were visualized using chemiluminescence (ECL; Biological Industries, Beit Ahemeq, Israel). The specific Caspase-3 inhibitor Z-DEVD-FMK (DEVD) and the tyrosine kinase inhibitor imatinib were obtained from Selleck Chemicals. The primary antibodies used were as follows: anti-BECLin1, anti-cleaved PARP, anti-cleaved Caspase-3, anti-Atg5, anti-Atg7, anti-UVRAG, anti-p-BCR/ABL (Tyr177), anti-Flag, anti-HA, and anti-GAPDH, purchased from Cell Signaling Technology (Beverly, MA, USA), and anti-BCR/ABL, anti-p62/SQSTM1, and anti-LC3 I/II, purchased from Abcam (Cambridge, UK).

Huang X., Li Y., Shou L., Li L., Chen Z., Ye X, & Qian W. (2019). The molecular mechanisms underlying BCR/ABL degradation in chronic myeloid leukemia cells promoted by Beclin1-mediated autophagy. Cancer Management and Research, 11, 5197-5208.

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Rapamycin Modulates Autophagy Markers

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The reagent rapamycin (Rapa; R0395; Sigma-Aldrich) was used in the study. The primary antibodies used were anti-ATG14 (no. 96752; Cell Signaling Technology), anti-UVRAG (no. 13115; Cell Signaling Technology), anti-BECN1 (no. 54101; Cell Signaling Technology), anti-p62 (P0067; Sigma-Aldrich), anti-LC3 (L8918; Sigma-Aldrich), anti-Bif1 (NBP2-24733; Novus), anti-GAPDH (RM2002; Beijing Ray Antibody Biotech), anti-RABV (5B12) (NB110-7542; Novus), and fluorescein isothiocyanate (FITC)-conjugated anti-RABV (800-092; FUJIREBIO). The secondary antibodies used were horseradish peroxidase (HRP)-conjugated goat antirabbit IgG (ab97051; Abcam) and goat antimouse IgG (sc-2005; Santa Cruz).

Hou P., Guo Y., Jin H., Sun J., Bai Y., Li W., Li L., Cao Z., Wu F., Zhang H., Li Y., Yang S., Xia X., Huang P, & Wang H. (2023). Bif-1c Attenuates Viral Proliferation by Regulating Autophagic Flux Blockade Induced by the Rabies Virus CVS-11 Strain in N2a Cells. Microbiology Spectrum, 11(3), e03079-22.

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Lysosome Inhibition and Protein Interaction

Cited 2 times

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The lysosome inhibitors NH₄Cl and leupeptin (Fisher Scientific) were used together at 20 mM and 200 µM respectively for 4 h. The lysosomal inhibitor concanamycin A (Sigma–Aldrich) was used at 1 µM for 30 min. Primary antibodies used for immunoprecipitation and Western blotting were as follows: anti-NRBF2 (Cell Signaling Technology), anti-Vps34 for immunoprecipitation [24 (link)], anti-Vps34 for Western blotting [25 (link)], anti-Vps15 [18 (link)], anti-Beclin-1 (BD Biosciences), anti- Atg14L (MBL International), anti-UVRAG (Cell Signaling Technology), anti-LC3 (Cell Signaling Technology), anti-p62 (MBL International), anti-V5 for immunoprecipitation (Thermo Scientific); anti-V5 for Western blotting (Life Technologies), anti-FLAG (Sigma–Aldrich); anti-actin (Sigma–Aldrich), anti-GAPDH (glyceraldehyde-3-phosphate dehydrogenase; MBL International) and anti-(rabbit IgG) (Jackson ImmunoResearch). Anti-HA (haemagglutinin) and anti-Myc antibodies were produced in-house.

Cao Y., Wang Y., Abi Saab W.F., Yang F., Pessin J.E, & Backer J.M. (2014). NRBF2 regulates macroautophagy as a component of Vps34 Complex I. The Biochemical journal, 461(2), 315-322.

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Antibodies and Reagents for Cell Signaling Studies

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Antibodies used in this study are as follows: anti-Vps34 (Cell signaling, #4263), anti-Beclin1 (Cell signaling, #3738; Santa Cruz, sc-48341 for immunoprecipitation), anti-ATG14L (MBL, PD016), anti-UVRAG (Cell signaling, #13115), LC3B (Cell signaling, #2775), α-tubulin (Santa Cruz, sc-23948), anti-vinculin (SIGMA, V9131) anti-Flag HRP conjugate (Sigma, A8592), anti-HA HRP conjugate (Cell signaling, #2999S) and the secondary antibodies conjugated with HRP, anti-Rabbit IgG-HRP (Bethyl, #A120-101P) and anti-Mouse IgG-HRP (Bethyl, #A90-116P). The affinity resins for the protein purification or immunoprecipitation were obtained from SIGMA (Flag-M2 agarose, A2426), GE Healthcare (GSH-Sepharose, 17-0756-01; Protein G-Sepharose, 17-0618-01), and Invitrogen (Protein A-Sepharose, #101041). Chloroquine (C6628), α-pinene (147524), Δ-3-carene (115576), α-cedrol (93483), β-caryophyllene (22075), and α-humulene (5375) were obtained from Sigma-Aldrich. High glucose (25 mM) DMEM (LM-001-07) and glucose-free DMEM (LM001-56) were from Welgene.

Jung J., Chun Y., Jang Y.P., Oh M.S., Kim J.H, & Kim J. (2021). An alcoholic extract of Thuja orientalis L. leaves inhibits autophagy by specifically targeting pro-autophagy PIK3C3/VPS34 complex. Scientific Reports, 11, 17712.

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