We cut 14 μm frozen sections from fly antennae and prepared and stained them as previously described [4 (link)]. We used the following antibodies: mouse anti-GFP (A11120, ThermoFisher Scientific) at 1:200, Alexa 488-conjugated anti-mouse IgG (ab150117, Abcam) at 1:850, Alexa 594-conjugated anti-rabbit IgG (A11012, ThermoFisher Scientific) at 1:850, the mouse monoclonal 21A6 (DSHB) at 1:100, and a polyclonal rabbit anti-Orco antibody (raised against the peptide SSIPVEIPRLPIKS by AbFrontier, South Korea) at 1:3000.
Alexa 488 conjugated anti mouse igg
Manufactured by Abcam
Sourced in United Kingdom
Alexa Fluor 488-conjugated anti-mouse IgG is a secondary antibody that binds to mouse primary antibodies. It is labeled with the Alexa Fluor 488 fluorescent dye, which can be detected using a green fluorescence channel.
Automatically generated - may contain errors
2 protocols using alexa 488 conjugated anti mouse igg
1
Immunostaining of Fly Antennae
2
Quantifying Anti-Nuclei Antibody Levels
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Anti-nuclei antibody (ANA) level was detected by indirect immunofluorescence assay. HK-2 cells were seeded in 12-well plate at 10^5 cells/ml. Cells were fixed with 4% paraformaldehyde and permeated in 0.1% tritonx-100. Mouse serum was collected and diluted in PBS for 20 times dilution then incubated with fixed HK-2 cells overnight. Alexa-488 conjugated anti-mouse IgG (Abcam, UK) was used to detect mouse anti-nuclei antibody level.
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