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Caspase 11

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Caspase-11 is a member of the caspase family of enzymes. Caspases are a group of proteolytic enzymes that play essential roles in cell death and inflammation. Caspase-11 is responsible for the activation of other caspases and the initiation of the inflammatory response.

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Lab products found in correlation

Caspase 3, by Cell Signaling Technology (2 mentions) Cleaved caspase 3, by Cell Signaling Technology (2 mentions) Ab125066, by Abcam (1 mentions) Sirt1, by Cell Signaling Technology (1 mentions) Mettl3, by Cell Signaling Technology (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Ripa buffer, by Solarbio (1 mentions) Irdye 680 conjugated anti mouse igg, by LI COR (1 mentions) Irdye800 conjugated anti rabbit igg, by LI COR (1 mentions) Total erk, by Cell Signaling Technology (1 mentions) P jnk, by Cell Signaling Technology (1 mentions) Bcl xl, by Cell Signaling Technology (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) P erk, by Cell Signaling Technology (1 mentions) Cofilin, by Cell Signaling Technology (1 mentions) Tubulin, by Cell Signaling Technology (1 mentions) Torin1, by Bio-Techne (1 mentions) Fam flica caspase 1 assay kit, by ImmunoChemistry Technologies (1 mentions) P ampk, by Cell Signaling Technology (1 mentions) Rapamycin, by Cell Signaling Technology (1 mentions)

3 protocols using caspase 11

1

Western Blot Analysis of Cellular Proteins

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Cells or tissues were lysed in RIPA Buffer (Solarbio, Beijing, China) containing proteinase inhibitor cocktail. Proteins were separated with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto polyvinylidene fluoride (PVDF) membranes. The membranes were blocked and incubated with primary antibodies including CitH3 (1:1000), MPO (1:1000), LC3B (1:1000), SQSTM1/p62 (1:1000), GPX4 (1:2000, ab125066, Abcam), cleaved caspase-3 (1:1000, 9661, Cell Signaling Technology), caspase-3 (1:1000, 9662, Cell Signaling Technology), caspase-11 (1:1000, 14340, Cell Signaling Technology), SIRT1 (1:1000), METTL3 (1:1000), β-Actin (1:3000, 3700, Cell Signaling Technology). Signals were detected with a ECL chemiluminescence kit (Tanon, Shanghai, China) after HRP-conjugated secondary antibodies incubation.
Qu M., Chen Z., Qiu Z., Nan K., Wang Y., Shi Y., Shao Y., Zhong Z., Zhu S., Guo K., Chen W., Lu X., Wang Z., Zhang H, & Miao C. (2022). Neutrophil extracellular traps-triggered impaired autophagic flux via METTL3 underlies sepsis-associated acute lung injury. Cell Death Discovery, 8, 375.
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2

Antibody-Based Protein Expression Analysis

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We used antibodies for p-JNK, total JNK, p-ERK, total-ERK, AceH3K9, H3K917Me3, Bcl-XL, Caspase3, cleaved Caspase 3, Caspase 11, ILβ and cytochrome C (Cell Signaling Technology); β-actin and BAX (Santa Cruz Biotechnology, Inc.). Secondary antibodies were IRDye680 conjugated anti-mouse IgG and IRDye680 conjugated anti-goat IgG, IRDye800 conjugated anti-rabbit IgG and IRDye800 conjugated anti-goat IgG (Rockland, PA) (LICOR); TRITC- and FITC-conjugated donkey anti–mouse, anti–goat, and anti–rabbit (Jackson ImmunoResearch Laboratories, Inc.), and DAPI (Life-Tech); Antibodies CD68 (e-Bioscience), JC-1 staining kit (Thermo Fisher Scientific), and TUNEL assay kit (TMR Tunel staining kit, Roche) JNK-inhibitor SP600125 (Calbiochem) and ERK–inhibitor PD98059 (Calbiochem).
Samanta S., Zhou Z., Rajasingh S., Panda A., Sampath V, & Rajasingh J. (2018). DNMT and HDAC inhibitors together abrogate endotoxemia mediated macrophage death by STAT3-JMJD3 signaling. The international journal of biochemistry & cell biology, 102, 117-127.
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3

Antibody-Mediated Analysis of Cellular Signaling

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Antibodies specific for CD11b were from BD Biosciences; p-rpS6, rpS6, p-AktS473, p-AktT308, Akt, p-AMPK, AMPK, tubulin, mTOR, LC3A, p-cofilin, cofilin, caspase-11, and FLAG (Cell Signaling); caspase-1 (Genentech); HA (Abcam); CD40, CD80, and anti-rabbit IgG (Biolegend Pharmaceuticals); anti-goat and -rabbit (Alexa 647) IgG (Molecular Probes); Concanamycin A (Sigma-Aldrich); LimKi 3 and Jasplakinolide (EMD Calbiochem); Torin1(Tocris); rapamycin (Cell Signaling); IRDye680- and IRDy800-conjugated antibodies (anti-rabbit, anti-mouse) (LI-COR Biosciences); phalloidin and LysoSensor Yellow/Blue dextran (ThermoFisher); Streptavidin (PE) (Jackson ImmunoResearch); and FAM-FLICA caspase-1 assay kit (ImmunoChemistry Technologies). Antibodies specific to nucleosome (PL2-3), Smith (2.12.3), and CD16/32 (2.4G2) and L-cell media (LCM) for BMMφ differentiation were generated as previously described (13 (link)).
Monteith A.J., Vincent H.A., Kang S., Li P., Claiborne T.M., Rajfur Z., Jacobson K., Moorman N.J, & Vilen B.J. (2018). mTORC2 activity disrupts lysosome acidification in systemic lupus erythematosus by impairing caspase-1 cleavage of Rab39a. Journal of immunology (Baltimore, Md. : 1950), 201(2), 371-382.
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