Human prostate adenocarcinoma PC3, derived from bone metastasis, and LNCaP, derived from lymph node metastasis, cells were purchased from the Korean Cell Line Bank. Cells were cultured in RPMI-1640 medium (Thermo Fisher Scientific) containing 10 mM D-glucose supplemented with 10% FBS (ATCC) and 1% penicillin/streptomycin in a 5% CO2 incubator at 37 °C. For mitogen stimulation, cells were serum-deprived for 24 h and treated with or without 10% FBS for 6 h. All cells were routinely tested for negative mycoplasma contamination using Mycoplasma Detection Kit (Lonza).
Mycoplasma detection kit
Manufactured by Lonza
Sourced in Switzerland, United States
The Mycoplasma Detection Kit is a laboratory equipment product designed to detect the presence of Mycoplasma, a type of bacteria, in cell cultures. The kit provides a reliable and efficient method for monitoring and ensuring the absence of Mycoplasma contamination in research and biopharmaceutical applications.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (15 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (11 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (6 mentions)
Mda mb 231, by American Type Culture Collection (6 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (6 mentions)
Fetal bovine serum (fbs), by Merck Group (5 mentions)
Rpmi 1640, by Thermo Fisher Scientific (4 mentions)
Du145, by American Type Culture Collection (3 mentions)
L glutamine, by Thermo Fisher Scientific (3 mentions)
Bt474, by American Type Culture Collection (3 mentions)
Mcf 7, by American Type Culture Collection (3 mentions)
Mda mb 453, by American Type Culture Collection (3 mentions)
Hek293t cell, by American Type Culture Collection (3 mentions)
Hek293t, by American Type Culture Collection (3 mentions)
Mda mb 468, by American Type Culture Collection (3 mentions)
Gentamicin, by Thermo Fisher Scientific (3 mentions)
Roswell park memorial institute (rpmi), by Thermo Fisher Scientific (3 mentions)
Glutamax, by Thermo Fisher Scientific (3 mentions)
Neurobasal a media, by Thermo Fisher Scientific (2 mentions)
Multigas incubator, by Sanyo (2 mentions)
90 protocols using mycoplasma detection kit
1
Cultivation of Prostate Cancer Cells
2
Ovarian Cancer Cell Lines Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The human ovarian cancer cell lines: MCAS and OVSAHO (JCRB cell bank, Osaka, Japan, catalog no. JCRB0240 and no. JCRB1046 respectively) were kindly provided by Prof. Aikou Okamoto (Jikei University School of Medicine, Japan), in 2016. The cisplatin sensitive A2780 (The European Collection of Authenticated Cell, ECACC catalog no. 93112519) and cisplatin-resistant A2780-cis (ECACC catalog no. 93112517) cell lines were a generous gift from Dr. Benjamin Tsang (University of Ottawa, Canada), in 2018. The transformed normal epithelial ovarian cell line HOSE6–3 (RRID: CVCL_7673), established by Prof. GSW Tsao (School of Biomedical Sciences, The University of Hong Kong), was kindly provided by his laboratory in 2018. To avoid contaminations, our cell-culture laboratories, including hoods and incubators, are systemically fumigated every year, and any new cells are tested upon arrival, for the presence of mycoplasma using the “Mycoplasma Detection Kit” (Lonza, Catalog #: LT07–118). None of the cells used for this study were tested positive.
MCAS, A2780, A2780 cis and HOSE6–3 cells were propagated in DMEM (Gibco, NY, USA), while OVSAHO was cultured in RPMI-1640 media supplemented with 10% FBS (Gibco, NY, USA) and 1% penicillin-streptomycin antibiotic (Gibco, NY, USA) in a humidified incubator at 37 °C and 5% CO2.
MCAS, A2780, A2780 cis and HOSE6–3 cells were propagated in DMEM (Gibco, NY, USA), while OVSAHO was cultured in RPMI-1640 media supplemented with 10% FBS (Gibco, NY, USA) and 1% penicillin-streptomycin antibiotic (Gibco, NY, USA) in a humidified incubator at 37 °C and 5% CO2.
3
Isolation and Characterization of Glioblastoma Neurospheres
4
Culture of Human Pancreatic Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human pancreatic duct epithelial cell lines H6c7, pancreatic carcinoma cell lines PANC-1 and SW-1990 (ATCC) were cultured in RPMI-1640 medium (Gibco) containing 10 mM D-glucose supplemented with 10% FBS (Gibco) and 1% penicillin/streptomycin in a 5% CO2 incubator at 37°C. All cell lines were routinely tested for negative mycoplasma contamination using Mycoplasma Detection Kit (Lonza).
5
Gallbladder Cancer Cell Line Culturing
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We used 5 GBC cell lines: TGBC2TKB, NOZ [25 (link)], GBd15 [26 (link)], TYGBK-1 [27 (link)], and TYGBK-8. TGBC2TKB was purchased from Riken Cell Bank (Tsukuba, Japan). NOZ, TYGBK-1, and TYGBK-8 were purchased from the Japanese Collection of Research Bioresources (JCRB) bank. All cell lines were cultured according to the supplier's specifications. There was no mycoplasma contamination in any of the cell lines with mycoplasma detection kit (Lonza, Basel, Switzerland). For normoxic conditions, cells were cultured in 5% CO2 and 95% air and for hypoxic conditions, cells were cultured in 1% O2, 5% CO2, and 94% N2, in a multigas incubator (Sanyo, Tokyo, Japan).
6
Culture of MNK-1, NKL, and KHYG-1 Cells
7
Culture of MNK-1, NKL, and KHYG-1 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MNK-1 cells40 (link) were cultured in DMEM supplemented with 10% FBS, 2 mM GlutaMAX, 1 mM sodium pyruvate, 55 μM 2-mercaptoethanol, 10 mM HEPES, 50 μg/ml gentamicin, 100 U/ml penicillin, and 100 μg/ml streptomycin (all from Corning or Gibco Life Technologies, except FBS from Atlanta Biologicals). To facilitate robust survival, 10 ng/ml recombinant mouse IL-2 (BioLegend) were re-applied daily. NKL cells41 (link) and KHYG-1 cells42 were cultured in RPMI 1640 medium supplemented with 2 mM L-glutamine, 1 mM sodium pyruvate, 100 U/ml penicillin, 100 μg/ml streptomycin and 7.5% FCS plus 7.5% new born calf serum (all from Corning or Gibco Life Technologies, except FBS from Atlanta Biologicals). The cell concentration was maintained at ~5 × 105 /ml in the presence of 50 U/ml of recombinant human IL-2 (Amgen). All cell lines routinely tested negative for mycoplasma contamination using the Mycoplasma Detection Kit (Lonza) and maintained in medium containing plasmocin (Invivogen) for prophylactic purpose.
8
Investigating NSCLC Cell Line Responses
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All drugs were purchased from Selleck Chemicals (Houston, TX) and prepared as 10 mmol/l stock solutions in dimethyl sulfoxide. Predesigned sets of four independent siRNA sequences of the target genes Plk1 and MET (siGENOME SMARTpool; Dharmacon, Lafayette, CO) were used. Human TGF‐β1 was purchased from Cell Signaling Technology (Danvers, MA). Human fibronectin was purchased from Sigma‐Aldrich (St. Louis, MO). Constitutive active TPR‐Met plasmid and control pBABE plasmid were obtained from Addgene (Cambridge, MA). Antibodies and dilutions used in the study are listed in Appendix Table S3 . The HGF neutralizing monoclonal antibody (24612.111) was acquired from Invitrogen (Catalog # MA1‐24767, Thermo Fisher Scientific, Waltham, MA).
Human NSCLC cell lines were obtained, maintained, and genotyped by STR profiling as previously described (Ferrarotto et al,2016 ; Peng et al, 2016 ) and routinely tested for the presence of mycoplasma species using the Mycoplasma Detection Kit (Lonza, Basel, Switzerland).
Human NSCLC cell lines were obtained, maintained, and genotyped by STR profiling as previously described (Ferrarotto et al,
9
Establishing Cell Culture Conditions for Breast Cancer Research
10
Murine models for cancer research
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Female C57BL/6J (6- to 8-week-old) mice and BALB/C-nu/nu mice were purchased from Joint Ventures Sipper BK Experimental Animal Co (Shanghai, China). Faslpr and Faslgld mice were purchased from the Jackson Laboratory (Farmington, CT, USA). Il9r−/−60 (link) mice were kindly provided by Dr. Jean-Christophe Renauld (Université Catholique de Louvain, France). Mice were housed in a specific pathogen-free facility, and experimental protocols were approved by the Animal Care and Use Committee of the School of Medicine, Zhejiang University. Murine B16F10 tumor cells and HEK293 cells were obtained from the American Type Culture Collection (Manassas, VA, USA). B16F10-OVA and LLC-OVA cells were provided by Dr. Qibin Leng (University of Chinese Academy of Sciences, Shanghai, China) and Wei Yang (Southern Medical University, Guangzhou, Guangdong, China), respectively. B16F10 and B16F10-OVA cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% (v/v) fetal calf serum (Lonza, Basel, Switzerland). LLC-OVA and HEK293 cells were cultured in RPMI-1640 medium supplemented with 10% fetal calf serum. All cells were routinely tested for mycoplasma contamination using the Mycoplasma Detection Kit (Lonza) and were found to be negative.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!