End-capped polymer buffering capacity as a function of polymer structure was assessed by titrating 10 mg (100 μL at 100 mg/mL) of polymer dissolved in 10 mL of acidified, 100 mM NaCl from pH 3.0 to pH 11.18 (link) For titrations, pH was determined using a SevenEasy pH Meter (Mettler Toledo) with pH assessed after stepwise addition of 100 mM sodium hydroxide.
Seveneasy ph meter
Manufactured by Mettler Toledo
Sourced in Switzerland, United States
The SevenEasy pH meter is a compact, easy-to-use device designed for reliable pH measurement. It features a digital display, automatic temperature compensation, and the ability to store up to 12 calibration points. The SevenEasy pH meter is suitable for a variety of applications where accurate pH measurement is required.
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Lab products found in correlation
Nicolet evolution 300 uv vis spectrophotometer, by Thermo Fisher Scientific (2 mentions)
Inlab routine pro ph probe, by Mettler Toledo (2 mentions)
Praying mantis accessory, by Harrick (2 mentions)
Spectralon baseline, by Harrick (2 mentions)
L isoleucine, by Merck Group (1 mentions)
L leucine, by Merck Group (1 mentions)
Ammonium acetate, by Merck Group (1 mentions)
Milli q, by Merck Group (1 mentions)
Pyrex, by Corning (1 mentions)
Matlab, by MathWorks (1 mentions)
Millex hv pvdf, by Merck Group (1 mentions)
Chemstation, by Hewlett-Packard (1 mentions)
Agilent 1100 hplc system, by Agilent Technologies (1 mentions)
Seven2go s3, by Mettler Toledo (1 mentions)
Ascorbate, by Merck Group (1 mentions)
Penicillin, by Harvard Bioscience (1 mentions)
Streptomycin, by Harvard Bioscience (1 mentions)
Fetal calf serum (fcs), by Harvard Bioscience (1 mentions)
Dmem f12, by Harvard Bioscience (1 mentions)
Collagenase 2 solution, by Harvard Bioscience (1 mentions)
36 protocols using seveneasy ph meter
1
Polymer Buffering Capacity Analysis
2
Amino Acid Standards Preparation
3
Measuring Redox Dynamics in Bacterial Cultures
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Hamilton oxidation-reduction potential (ORP) probes (Hamilton Company, Reno, NV, USA) were inserted into air-tight Pyrex (Corning Inc, Corning, NY, USA) bottles containing mMRS supplemented with 20 μg/mL DHNA and/or 1.25 mM ferric ammonium citrate and incubated in a water bath at 37 °C. A custom cap for the Pyrex bottles was 3D printed with polylactic acid filament (2.85 mm diameter) such that the ORP probe threads into the cap and an o-ring seal can be used to provide an air-tight seal between the probe and the cap. The ORP was allowed to equilibrate over 40 min before L. plantarum NCIMB8826, Δndh2 (MLES100), or ΔpplA (MLES101) were inoculated at an OD600 of 0.10. Two uninoculated controls were used to measure baseline ORP over time. The ORP data was collected via Modbus TCP/IP protocol (Stride Modbus Gateway, AutomationDirect, Cumming, GA, USA) into a database (OSIsoft, San Leandro, CA, USA) and analyzed in MATLAB (Mathworks, Nantick, MA, USA). pH was measured using a Mettler Toledo SevenEasy pH meter (Mettler Toledo, Columbus, OH, USA). Cells were collected at either 24 hr or at the greatest ORP difference between the wild-type and mutant strains (ΔmVmax) by centrifugation at 10,000 g for 3 min and used for ferrihydrite reduction analyses.
4
pH Measurement of Diluted Samples
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Two batches of diluted samples (1:5 with DDW) were measured for each system using the Seven Easy pH meter (Seven Easy pH meter, Mettler Toledo, Greifensee, Switzerland).
5
Standardized pH Measurement Protocol
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pH measurements were carried out using Mettler Toledo Seven Easy pH meter (model S20) which was routinely calibrated with standard buffers. The experiments were performed at the 20 mM pH 7.4 sodium phosphate buffer. All preparations used in the experiments were filtered through 0.45 μm Millipore Millex-HV PVDF filter.
6
HPLC Analysis of Forced Degradation Samples
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Samples were analyzed on an Agilent 1100 HPLC system (Agilent Technologies, Palo Alto, CA, USA), attached with a G1311A quaternary pump, a G1312A vacuum degasser, and a G1315B DAD detector. The detector wavelength was fixed at 246 nm and the peak areas were integrated automatically using the Hewlett–Packard ChemStation software program [16 (link)]. Other apparatus included an ultrasound generator and a SevenEasy pH meter (Mettler Toledo, USA) that was equipped with a combined glass–calomel electrode. A Diamonsil C18 column (150 mm × 4.6 mm, 5 μm) was maintained at 30 °C. The mobile phase was composed of a phosphate buffer (10 mM, pH 3.5) containing 0.5% triethlamine and methanol (85:15, v/v). The flow rate of the mobile phase was set at 1 mL/min. Measurements were made with 20 μL of injection volume. For the analysis of the forced degradation samples, the photodiode array detector was used in a scan mode with a range of 200–400 nm. The peak homogeneity was expressed in terms of peak purity factor and was obtained directly from the spectral analysis report using the above-mentioned software.
7
Honey pH Measurement Protocol
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The solution containing honey in water (20% w/v) on a dry matter basis was prepared for pH measurement. The moisture content of a honey sample determined by the refractometer (described in Moisture content) was used to calculate the correct dilution. The measurement was done using a SevenEasy™ pH meter (Mettler Toledo, Urdorf, Switzerland) and InLab 427 electrode (Mettler Toledo). The pH standards of 4.01 ± 0.02 and 7.01 ± 0.02 were used for calibration before each measurement.8
8
Optimizing Cell Culture Media pH
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To acidify the media pH, 1 M HCl and lactic acid were treated in the media dose-dependent manner and incubated at 37 °C 5% CO2 condition for 24 h. After stabilization, pH of the media was immediately measured using a SevenEasy pH meter (Mettler Toledo, Columbus, OH, USA). The pH of the cultured media was measured immediately after the experiments using a SevenEasy pH meter, and the analyzed pH of the cultured media was summarized in Supplementary Tables S4–S6 .
9
Comprehensive Sediment Characterization Protocol
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The salinity of seawater was tested using an optical salinometer (Nanbei). The 1:2.5 sediment/water (dH2O) suspensions were shaken for 30 min before measuring the pH using a SevenEasy pH meter (Mettler Toledo). Similarly, the 1:5 sediment/water (dH2O) suspensions were shaken for 30 min before measuring the concentration of soluble salt. The salinity of sediment, which was determined by the Seven2Go S3 (Mettler Toledo), was determined to measure the amount of soluble salt. The contents of carbon, nitrogen, hydrogen, and sulfur were measured using a Vario EL cube V3.1.8 in CHNS mode (Elementar Analysensysteme GmbH) after the sediment was air‐dried and filtered using a 200‐mesh screen. We used 20–50 mg sediment of each sample for this measurement. The temperatures of the combustion furnace and the reduction furnace were set to 1150°C and 850°C, respectively. The CO2 and SO2 column desorption temperatures were set to 240°C and 220°C, respectively. The time duration of oxygenation was 120 s and the total test time for a sample was 10 min. The standard sample used for calibration was sulfanilic acid.
10
pH Measurement of Tea Samples
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The pH measurements of tea samples were taken by following the methods from Ref. [12 ] by using a Seveneasy pH meter (Mettler-Toledo, Switzerland). Fresh standard buffer solutions of pH 4.0, 7.0, and 9.21 were used to calibrate pH meter before it was used at room temperature. The pH readings were made by triplicates and the findings were recorded as average.
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