Cell proliferation was valued by the Cell Counting Kit-8 (CCK-8) Assay Kit (Vazyme, Nanjing, China). The KTC-1 and TPC-1 cells were cultured in 96-well plates with 1.5 × 103 cells/well; 10 μl of CCK-8 reagent was added to each well and then incubated for 3 h in a dark environment. A microplate reader was used to measure the absorbance at 450 nm. The proportion of relative cell proliferation through the average optical density (OD) of each group was calculated. The formula is as follows: (OD treatment/OD control) × 100%.
Cell counting kit 8 cck 8 assay kit
Manufactured by Vazyme
Sourced in China
The Cell Counting Kit-8 (CCK-8) Assay Kit is a colorimetric assay used for the determination of cell viability and cytotoxicity. It employs the water-soluble tetrazolium salt WST-8, which is reduced by dehydrogenases in viable cells to produce a yellow-colored formazan dye. The amount of the formazan dye is directly proportional to the number of living cells, allowing for the quantification of cell proliferation and cytotoxicity.
Automatically generated - may contain errors
6 protocols using cell counting kit 8 cck 8 assay kit
1
Cell Proliferation Assay with CCK-8
2
Cell Proliferation Assay using CCK-8
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell proliferation was valued by the Cell Counting Kit-8 (CCK-8) Assay Kit (Vazyme, Nanjing, China). The KTC-1 and TPC-1 cells were cultured in 96-well plates with 1.5 × 103 cells/well\. A microplate reader was measured in the absorbance at 450 nm. The proportion of relative cell proliferation through the average optical density of each group was calculated. The formula is as follows: (OD treatment/OD control) × 100%.
3
Colorimetric Glucose Assay for BMEC Metabolism
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A colorimetric glucose oxidase/peroxidase assay kit (E1010-1, Applygen, China) was used to determine the amount of glucose present in the culture medium. The amount of glucose absorbed was estimated as the difference between the amounts before and after 6-h incubation and presented as miligrams of glucose per miligram of protein per hour (Silva et al., 2022 (link)). A BCA Protein Assay Kit (P0012, Beyotime, Beijing, China) was used to assess the total protein content of the cells, and a Cell Counting Kit-8 (CCK-8) assay kit (A311, Vazyme Biotech Co., Ltd, China) was used for the estimation of cell proliferation, as previously described (Yang et al., 2022 ). The HK1KO, HK2KO, and wild-type BMEC were seeded into 96-well plates at 1250, 2500, 5000, 10,000, and 20,000 cells per well to generate a standard curve. After treatment, the cells were washed five times with 200 μL of sterile water and then cultured in 100 μL of DMEM supplemented with 10 μL of CCK-8 reagent at 37 °C with 5% CO2 for 3 h. The absorbance of each well at 450 nm was determined using an automated microplate reader (Multiskan GO, Thermo Scientific, Shanghai, China).
4
Assessing Erastin-Induced Cell Viability
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After 6 h of siRNA transfection, HK-2 cells were plated in 96-well plates (5,000 cells per well) and incubated for 24 h. Then cells were treated with 15 μM of erastin for another 24 h. Cell viability was assessed by Cell Counting Kit-8 (CCK-8) Assay Kit (Vazyme, Nanjing, China). After treatment with erastin, 10 μl CCK-8 was added to each well and incubated at 37°C for 2 h. The optical density value of each well was measured with a microplate reader (Bio-Tek, United States) at 450 nm.
5
Cell Viability Evaluation via CCK-8 Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell viability was evaluated using the Cell Counting Kit-8 (CCK 8) Assay Kit (Vazyme, China). 5 × 103 H9c2 cells/well were cultured in 96-well plates and incubated overnight. After the cells achieved 70% confluence, they were incubated with different LPS or neferine concentrations (5, 10, 20, and 50 μg/ml) for 24 h. Subsequently, 10 μl CCK-8 solution was added to each well. After incubation at 37°C for 2 h, the absorbance was measured at 450 nm.
6
Measuring Cell Viability and Death
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell death was measured by PI staining coupled with microscopy. PI-staining positive cells were observed using fluorescence microscopy. Cell viability was measured using the Cell Counting Kit-8 (CCK 8) Assay Kit (Vazyme, China). Briefly, 5 × 103 H9c2 cells/well were cultured in 96-well plates and incubated overnight. After the cells achieved 70% confluence, they were treated according to different experimental requirements. Subsequently, 10 μl CCK-8 solution was added to each well. After incubation at 37 °C for 2 h, the absorbance was measured at 450 nm.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!