Apoptosis was determined by flow cytometry analysis using the Annexin V-FITC/PI Apoptosis Kit (Becton Dickinson, Franklin Lakes, NJ, United States). Cells were seeded in 6-well plates (5 × 105 cells/well), digested with trypsin (Gibco trypsin-EDTA; Thermo Fisher Scientific), washed with phosphate-buffered saline (PBS) three times, suspended in 500 μl of binding buffer, and then incubated with 5 μl of fluorescein isothiocyanate (FITC)-conjugated Annexin V and 3 μl of propidium iodide (PI) for 15 min at room temperature in the dark. After incubation, the samples were tested using a flow cytometer (Moflo XDP, United States). Early apoptotic chondrocytes were defined as annexin V-positive and PI-negative cells, and late apoptotic chondrocytes were defined as annexin V-positive and PI-positive cells. The proportion of apoptotic cells was calculated using the FASC Calibur MT flow cytometer (BD Bioscience, NJ, United States).
Annexin 5 fitc pi apoptosis kit
Manufactured by BD
Sourced in United States
The Annexin V-FITC/PI apoptosis kit is a laboratory reagent used for the detection and analysis of apoptosis, a form of programmed cell death, in cell cultures. The kit contains Annexin V conjugated with the fluorescent dye FITC and the DNA-binding dye propidium iodide (PI). These reagents enable the identification of cells in different stages of apoptosis by flow cytometry or fluorescence microscopy.
Automatically generated - may contain errors
Lab products found in correlation
Facscalibur flow cytometer, by BD (9 mentions)
Facscalibur, by BD (8 mentions)
Facscanto 2, by BD (3 mentions)
Facs vantage system, by BD (2 mentions)
Lsr 2 flow cytometer, by BD (2 mentions)
Facs flow cytometer, by BD (2 mentions)
Bcl 2, by Cell Signaling Technology (2 mentions)
Accuri c6, by BD (2 mentions)
Gibco trypsin edta, by Thermo Fisher Scientific (1 mentions)
Fasc calibur mt flow cytometer, by BD (1 mentions)
Mem culture medium, by Thermo Fisher Scientific (1 mentions)
Jc 1 probe, by Solarbio (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Cell counting kit 8 cck 8, by Dojindo Laboratories (1 mentions)
Trypsin, by Solarbio (1 mentions)
Formic acid, by Tedia (1 mentions)
High performance liquid chromatography grade acetonitrile, by Tedia (1 mentions)
Oligomycin, by Agilent Technologies (1 mentions)
Glut4, by Cell Signaling Technology (1 mentions)
88 protocols using annexin 5 fitc pi apoptosis kit
1
Annexin V-FITC/PI Apoptosis Assay
2
Quantifying Cell Apoptosis by Flow Cytometry
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Apoptosis rates of cells were measured using an Annexin V-FITC/PI apoptosis kit (Becton Dickinson, Franklin Lakes, NJ, USA) following the manufacturer's instructions. Briefly, cells were harvested, washed with cold PBS, and resuspended in binding buffer. Then, 5 µl Annexin V-FITC and 5 µl PI were added to each sample containing 1×105 cells/100 µl. The samples were incubated at 25°C in the dark for 15 min, followed by addition of 400 µl binding buffer. Within 1 h of preparation, the samples were analyzed by a flow cytometer (Becton Dickinson). The experiments were repeated three times independently.
3
Apoptosis Quantification by Annexin V-FITC/PI
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell apoptosis was detected following specific treatments by staining using the Annexin V-FITC/PI apoptosis kit (Becton Dickinson, San Jose, CA, USA) according to the manufacturer's instructions. Briefly, cells were harvested after treatment and resuspended in binding buffer. Aliquots of 105 cells were mixed with 5 μL each of annexin V-FITC and PI solution for 15 min at room temperature in the dark. After incubation, 400 μL binding buffer was added, and cells were analyzed by FACS-Calibur flow cytometer (Becton Dickinson). Cell population in the lower left quadrant (Annexin V-/PI-) represents the live cells, population in the lower right quadrant (Annexin V+/PI-) represents the early apoptotic cells, and population in the upper right quadrant (Annexin V+/PI+) represents the late apoptotic or dead cells.
4
Annexin V-FITC/PI Apoptosis Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell apoptosis was analyzed using the Annexin V-FITC/PI Apoptosis kit (BD Biosciences, Franklin Lakes, NJ) according to the manufacturer's protocols. Cells were seeded in 6-well plates at a density of 2×105 cells/well. After indicated treatments, the cells were collected and washed twice with cold PBS, and then resuspended in a binding buffer containing Annexin V-FITC and propidium iodine (PI). After incubation for 15 min at room temperature in the dark, the fluorescent intensity was measured using a FACSCalibur flow cytometer (BD Biosciences, Franklin Lakes, NJ).
5
Apoptosis Analysis of Compound-1H in U87 and LN229 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Apoptosis induced by the compound-1H in U87 and LN229 cells was analyzed with a FACS using the Annexin V-FITC/PI apoptosis kit (BD, 556547). Briefly, after 48 h of the compound-1H treatment, U87 and LN229 cells were harvested and then resuspended with 1× binding buffer to a cell density of 1 × 106 cells/mL. Subsequently, the cells were stained with 5 μL Annexin V-FITC and 5 μL PI (50 μg/mL) and then incubated in the dark room for 15 min. The stained cells were diluted with 1× binding buffer to appropriate density, and immediately detected using FACS. The percentage of apoptotic cells was obtained using FACS.
6
Annexin V-FITC/PI Apoptosis Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Using an Annexin V-FITC/PI apoptosis kit (BD Pharmingen, USA), the apoptosis of treated RIN-m5F cells was assessed following the manufacturer’s instructions. The cells were gathered and twice washed in phosphate-buffered saline (PBS). After that, cells were incubated in 500 μL of 1× binding buffer containing 5 μL of Annexin V-fluorescein isothiocyanate (FITC) and 10 μL of propidium iodide (PI) for 5 min in the dark. The next step was to identify apoptosis within 1 h using a flow cytometer (BD Biosciences, USA), and to generate the plot, the values for Annexin V and PI were placed as the horizontal and vertical axes, respectively.
7
Apoptosis Measurement in Hepatocellular Carcinoma
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell apoptosis measurement was performed according to protocol described previously [23 (link)]. Briefly, after treatment Bel 7402 and SMMC 7721 cells with different concentrations of XAG, cells were stained with Annexin V-FITC/PI apoptosis kit (BD Pharmingen, NJ, USA) in the dark for 15 min. Apoptotic cell ratio was detected using a flow cytometer (Beckman Coulter Inc., FL, USA).
8
Apoptosis Induction and Measurement
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After 48 h of transfection, cells were collected for the apoptosis assay. BH3 hydrochloride (MedChemExpress, Shanghai, China), an apoptosis-inducing polypeptide, which induces apoptosis by activating Bax or neutralizing Bcl-2 [34 (link)], was used for apoptosis induction. An AnnexinV-FITC/PI apoptosis kit (BD bioscience, USA) was applied in this study following the manufacturer’s protocol. Each assay was repeated three times. Student’s t-test was used to examine the statistical significance between the experimental group and the control group.
9
Ceftiofur Sodium Cytotoxicity Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Ceftiofur sodium (CEF, purity > 98%, CAS# 104010-37-9) was purchased from Meilunbio (China). Desfuroylceftiofur (DFC) was obtained from Toronto Research Chemicals (Canada). RPMI 1640, DMEM and MEM culture medium were provided by Gibco (USA). Fetal bovine serum (FBS) was purchased from BI (Israel). Cell Counting Kit-8 (CCK-8) was obtained from Dojindo (Japan). Hoechst 33342/PI fluorescent dye, JC-1 probe and trypsin were provided by Solarbio (China). Annexin V-FITC/PI apoptosis kit was purchased from BD (USA). High-performance liquid chromatography grade acetonitrile and formic acid were obtained from Tedia (USA).
10
Quantifying Apoptosis in HSFs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell apoptosis was detected with an Annexin V-FITC PI Apoptosis Kit (BD Biosciences, CA, USA). After coculture for 24 h to 48 h, the HSFs were collected and resuspended in a flow tube with 1 × Annexin V Binding Buffer, and Annexin V-FITC and PI were added according to the instructions. After incubation for 15 min, the cells were detected with a FACSCalibur instrument (BD Biosciences, CA, USA). Data were analyzed using FlowJo software (Tree Star, Inc., Ashland, OR, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!