Dimension rxl

For the evaluation of the potentially toxic and side effects of EO formulation, a clinical examination of tested animals was conducted at D0, D7, and D14, with special emphasis on general condition, animal behavior, feeding, and defecation. Additionally, blood samples from the jugular vein were taken from tested animals at D0 and D14 into a vacuum tube containing EDTA and used for the evaluation of toxic effects on blood count, whereby the analyses were conducted shortly thereafter (2–4 h after taking samples). Blood samples on the same days were also taken for biochemical analyses, for the evaluation of the effects of the applied EO formulation on the kidneys (parameters urea and creatinine) and the liver (activity of the enzymes aspartate aminotransferase (AST) and gamma-glutamyl transferase (GGT)).
A clinical chemistry system (Dimension RXL, Dade Behring, Ventura, CA, USA) was used to quantify urea, creatinine, and enzymes AST and GGT in serum samples. The white blood cell count, red blood cell count, hemoglobin, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, platelets, lymphocytes, monocytes, neutrophil granulocytes, eosinophils granulocytes, and basophils granulocytes were determined in blood samples using an electronic particle counter (MS9; Melet Schloesing Laboratoires, Osny, France).

GDS and GDR clones were cultured in 6-well plates under normal conditions and after 72 h supernatants were collected. Glucose and lactate concentrations were determined by colorimetric methods on an automated analyzer (Dimension RxL, Dade Behring, Milan, Italy). Values were normalized to cells number at the end of the incubation period.

Each subject underwent phlebotomy to obtain ca. 25 ml of venous whole blood from a cubital vein. Total cholesterol, triglycerides, LDL and HDL cholesterol concentrations were determined using a Dimension RxL unit (Dade-Behring, Germany). If triglyceride concentrations were < 4.6 mmol/liter and chylomicronemia was not reported, the LDL cholesterol concentration was calculated using the Friedewald formula [26 (link)]:
$\begin{array}{l}\mathit{LDL}\mathit{cholesterol}=\left(\mathit{Total}\mathit{cholesterol}-\mathit{triglycerides}\right)\\ /\left(2.2-\mathit{HDL}\mathit{cholesterol}\right)\end{array}$
Lipid concentrations are given as mmol/liter (in order to convert HDL, LDL and total cholesterol concentrations to mg/dl, the molar value is multiplied by 38.67; for triglycerides, the multiplication factor is 87.5). Normal values were based on the reference range of the Department of Clinical Chemistry of the University of Ulm: total cholesterol < 5 mmol/l; LDL cholesterol < 3 mmol/l; HDL cholesterol > 1.2 mmol/l in women and > 1.0 mmol/l in men; triglycerides < 1.7 mmol/l.