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Aldh1l1 creert2 transgenic mice

Manufactured by Jackson ImmunoResearch
Sourced in United States

Aldh1l1-CreERT2 transgenic mice express a tamoxifen-inducible Cre recombinase under the control of the aldehyde dehydrogenase 1 family, member L1 (Aldh1l1) promoter. This mouse line can be used for cell type-specific gene manipulation in astrocytes.

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2 protocols using aldh1l1 creert2 transgenic mice

1

Lineage Tracing of Aldh1l1+ Cells

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All animal experiments were performed in accordance with the guidelines of the Institutional Animal Care and Use Committee of University (Approval number: 2018–059). The protocol was reviewed and approved by the Ethics Committee on Laboratory Animal Care. The mice were housed in rooms with controlled 12 hr light/dark cycles, temperature, and humidity, and food and water were provided ad libitum. Eight- to 10-week-old C57BL/6 mice weighing 22–26 g were obtained from the Vital River Laboratory Animal Technological Company (Beijing, China). Aldh1l1-CreERT2 transgenic mice (Stock number #029655), Rpl22 lsl-HA (Ribotag) mice (Stock number #011029), and Rosa26lsl-YFP mice (Stock number #006148) were obtained from The Jackson Laboratory (Bar Harbor, ME, USA). Aldh1l1-CreERT2 mice were used for breeding to the Rpl22lsl-HA mice or Rosa26lsl-YFP mice. Eight- to 10-week-old Aldh1l1-CreERT2;Rpl22lsl-HA and Aldh1l1-CreERT2;Rosa26lsl-YFP mice were used for lineage-tracing experiments.
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2

Astrocyte Lineage Tracing in PD Model

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All animal experiments were performed in accordance with the guidelines of the Institutional Animal Care and Use Committee of University. The protocol was reviewed and approved by the Ethics Committee preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
The copyright holder for this this version posted November 18, 2021. ; https://doi.org/10.1101/2021.11.12.468309 doi: bioRxiv preprint PTBP1 repression fails to convert reactive astrocytes to dopaminergic neurons in a PD model 4 on Laboratory Animal Care. The mice were housed in rooms with controlled 12 hours light/dark cycles, temperature, and humidity, and food and water were provided ad libitum. Eight-to ten-week-old C57BL/6 mice weighing 22-26g were obtained from the Beijing Vital River Laboratory Animal Technological Company (Beijing, China). Aldh1l1-CreER T2 transgenic mice and Rpl22 HA/HA mice (Ribotag) were obtained from The Jackson Laboratory (Stock number #029655, #011029). Hemizygous Aldh1l1-CreER T2 males or females were used for breeding to the Rpl22 HA/HA mice. Aldh1l1-CreER T2 : Ribotag mice aged at 8-10 weeks were used for lineage tracing experiments.
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