Cadmium oxide (99.5%, powder), 2-ethylhexanoic acid (2-EHA, 99%), 1-octadecene (ODE, technical grade, 90%), oleylamine (OLA, technical grade, 70%), hexadecylamine (technical grade, 90%), trioctylamine (98%), trioctylphisphine (TOP, technical grade, 97%), selenium powder (powder, 100 mesh, 99.5%), zinc oxide (puriss, 99–100%), thiourea (TU, ACS Reagent,>99%), triethylene glycol dimethyl ether (TEGDME, 99%), hexadecylamine (technical grade, 90%), palmitic acid (BioXtra, ≥99%) zinc acetate dihydrate (ACS reagent, ≥99.0%), magnesium acetate tetrahydrate (ACS reagent, ≥98%), aluminium chloride hexahydrate (99%), gallium nitrate hydrate (99.9%), lithium chloride (anhydrous, ACS reagent, ≥99%), tetramethylammonium chloride (TMACl, reagent grade, ≥98%), potassium hydroxide (reagent grade, 90%), dimethyl sulfoxide (DMSO, anhydrous, ≥99.9%), and monoethanolamine (MEA, ACS reagent, ≥99.0%) were purchased from Sigma-Aldrich. n-Hexadecylphosphonic acid (97%) was from PlasmaChem GmbH. Methyl acetate (extra pure, 99%) was from Acros. Hexane, n-octane, toluene, acetone, methanol, ethanol, n-butanol, and isopropyl alcohol of spectroscopy grade were purchased from the local supplier Ekos-1. All reagents were used as received, without purification.
Magnesium acetate tetrahydrate
Manufactured by Merck Group
Sourced in United States
Magnesium acetate tetrahydrate is a chemical compound commonly used as a laboratory reagent. It has the chemical formula Mg(CH3COO)2·4H2O. The compound is a crystalline solid that is soluble in water and other polar solvents. It is often used in various chemical and analytical applications, but a detailed description of its specific functions or intended uses cannot be provided in an unbiased and factual manner without the risk of extrapolation.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulfoxide (dmso), by Merck Group (5 mentions)
Zinc acetate dihydrate, by Merck Group (4 mentions)
1 octadecene, by Merck Group (4 mentions)
Ammonium acetate, by Merck Group (4 mentions)
Sodium chloride (nacl), by Merck Group (3 mentions)
N n dimethylformamide (dmf), by Merck Group (3 mentions)
Potassium hydroxide, by Merck Group (2 mentions)
Monoethanolamine, by Merck Group (2 mentions)
Adenosine 5 triphosphate atp disodium salt hydrate, by Merck Group (2 mentions)
4 2 hydroxyethyl piperazine 1 ethanesulfonic acid hepes, by Merck Group (2 mentions)
Kinase glo kit, by Promega (2 mentions)
Ammonium hydroxide, by Merck Group (2 mentions)
Formic acid, by Merck Group (2 mentions)
Ethanol, by Merck Group (2 mentions)
Sulfur, by Merck Group (2 mentions)
1 octanethiol, by Merck Group (2 mentions)
Cadmium oxide, by Merck Group (2 mentions)
Agarose, by IBI Scientific (2 mentions)
Tetramethylammonium chloride, by Merck Group (1 mentions)
Palmitic acid, by Merck Group (1 mentions)
21 protocols using magnesium acetate tetrahydrate
1
Colloidal Synthesis of Inorganic Nanomaterials
2
Inhibition of AChE and GSK-3β Activities
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Acetylcholine iodide (ACh),
butyrylthiocholine chloride (BCh), electric eel AChE (EC 3.1.1.7),
horse serum BChE (EC 3.1.1.8), 5,5′-dithiobis[2-nitrobenzoic
acid], quercetin, and berberine were purchased from E. Merck, Fluka,
or Sigma, unless otherwise stated. BACE1 fluorescence resonance energy
transfer (FRET) assay kits (β-secretase) were purchased from
Pan Vera Co. (Madison, WI). Human recombinant GSK-3β was obtained
from ProSpec (ProSpec-Tany TechnoGene Ltd., Ness-Ziona, Israel). The
GSM substrate mimicking muscle glycogen synthase was purchased from
Merck Millipore (Millipore Corporation, CA). Kinase-Glo kits were
obtained from Promega (Promega Corporation, Madison, WI). Adenosine
5-triphosphate (ATP) disodium salt hydrate, ammonium hydroxide, 4-(2-hydroxyethyl)
piperazine-1-ethanesulfonic acid (HEPES), ammonium acetate, formic
acid, ethylene glycol-bis(aminoethylether)-N,N,N,N-tetra acetic acid
tetra sodium salt (EGTA), dimethyl sulfoxide (DMSO), ethylenediaminetetraacetic
acid (EDTA), amyloid-β protein fragments (Aβ1–40/42), thioflavin T, 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP), and magnesium
acetate tetrahydrate were purchased from Sigma-Aldrich (St. Louis,
MO). All chemicals and solvents from commercial sources were of reagent
grade.
butyrylthiocholine chloride (BCh), electric eel AChE (EC 3.1.1.7),
horse serum BChE (EC 3.1.1.8), 5,5′-dithiobis[2-nitrobenzoic
acid], quercetin, and berberine were purchased from E. Merck, Fluka,
or Sigma, unless otherwise stated. BACE1 fluorescence resonance energy
transfer (FRET) assay kits (β-secretase) were purchased from
Pan Vera Co. (Madison, WI). Human recombinant GSK-3β was obtained
from ProSpec (ProSpec-Tany TechnoGene Ltd., Ness-Ziona, Israel). The
GSM substrate mimicking muscle glycogen synthase was purchased from
Merck Millipore (Millipore Corporation, CA). Kinase-Glo kits were
obtained from Promega (Promega Corporation, Madison, WI). Adenosine
5-triphosphate (ATP) disodium salt hydrate, ammonium hydroxide, 4-(2-hydroxyethyl)
piperazine-1-ethanesulfonic acid (HEPES), ammonium acetate, formic
acid, ethylene glycol-bis(aminoethylether)-N,N,N,N-tetra acetic acid
tetra sodium salt (EGTA), dimethyl sulfoxide (DMSO), ethylenediaminetetraacetic
acid (EDTA), amyloid-β protein fragments (Aβ1–40/42), thioflavin T, 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP), and magnesium
acetate tetrahydrate were purchased from Sigma-Aldrich (St. Louis,
MO). All chemicals and solvents from commercial sources were of reagent
grade.
3
Fluorescent DNA Strand Preparation
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All DNA strands including Cy5 and Cy3 modified ones were bought from Sangon (Shanghai, China, www.sangon.com ), which were purified by denaturing polyacrylamide gel electrophoresis (PAGE). Strands were dissolved at a concentration of approximately 300 μM in nuclease free water. Actual strand concentrations were determined by UV/vis detection of DNA absorbance at 260 nm. The enzymes T4 DNA ligase and exonuclease I were purchased from Takara Biotechnology Co., Ltd. (Dalian, China). Water (18 MΩ cm) was from a Milli-Q Ultrapure Water Purification System (Merck Millipore, Shanghai, China). Ethylenediaminetetraacetate (EDTA), urea, magnesium acetate tetrahydrate, boric acid, acetic acid, and tris(hydroxymethyl)aminomethane (Tris) were purchased from Sigma Aldrich Corp. (Shanghai, China). The TBE buffer is composed of 89 mM Tris, 89 mM boric acid, and 2 mM EDTA at pH 8.3, and the TAE-Mg buffer is composed of 40 mM Tris, 2 mM EDTA and 12.5 mM Mg(Ac)2 at pH 6.0 ± 0.5.
4
Protein Translation Buffer Optimization for Force Measurements
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Force measurements were performed in various protein translation buffers [2 (link),11 (link)–12 (link)] in a microfluidic cell. All chemicals used in the buffer preparation were of a high purity grade and were used as received without further purification. Sodium chloride (≥98%), HEPES (BioUltra ≥99.5%), magnesium acetate tetrahydrate (99%), ammonium acetate (98%), 2-mercaptoethanol (BioUltra ≥99.0%) and DL-dithiothreitol (BioUltra ≥99.5%) were provided from Sigma-Aldrich (Dublin, Ireland). All aqueous solutions were prepared using nanopure water from a Millipore Milli-Q system, additionally filtered with 0.22 µm pore size filters. We used TICO buffer (20 mM HEPES-KOH, pH 7.5, 6 mM magnesium acetate, 30 mM ammonium acetate, 4 mM 2-mercaptoethanol) to examine various coupling ratios of PDHs to anti-DIG beads for the subsequent biotin-streptavidin binding in OT experiments. All experiments were also performed in TICO buffer with an increased Mg content of 12 mM (high Mg TICO). Control experiments were carried out in standard buffer (150 mM NaCl, 10 mM HEPES, pH 7.5) and in DTT buffer (40 mM HEPES-KOH, pH 7.5, 60 mM NH4Cl, 10 mM magnesium acetate, 1 mM DTT and 3.6 mM 2-mercaptoethanol) (adapted from [12 (link)]).
5
Perovskite Solar Cell Fabrication
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Zinc oxide dispersion,
tin chloride (SnCl2·2H2O) dehydrate, Al2O3 (aluminum oxide) NPs with <50 nm particle
size, magnesium acetate tetrahydrate [(CH3COO)2Mg·4H2O], lead(II) bromide (PbBr2), cesium iodide (CsI), tert-butylpyridine (TBP), Li bis(trifluoromethanesulfonyl)imide
(Li-TFSI), potassium hydroxide solution (KOH), and solvents dimethyl
sulfoxide (DMSO anhydrous, ≥99.9%), N,N-dimethylformamide (DMF anhydrous, 99.8%), diethyl ether
(99.0%), ethanol (99.8%), and 2-propanol anhydrous 99.5% were purchased
from Sigma-Aldrich. Tin(IV) oxide 15% in H2O colloidal
dispersion and 1-benzyl-3-methylimidazolium chloride (IL1) were purchased
from Alpha Aesar. 1-Butyl-3-methylimidazolium tetrafluoroborate (IL2)
was purchased from Acros Organics. Lead(II) iodide (PbI2) (99.99%, trace metal basis) was purchased from TCI Deutschland
GmbH. Formamidinium iodide (FAI) methylammonium bromide (MABr), and
methylammonium iodide (MAI) were purchased from GreatCell Solar. 18NR-T
titania paste and cobalt salt (III) FK209 TFSI (98%) were purchased
from Dyesol Limited. 2,2′,7,7′-Tetrakis-(N,N-di-p-methoxyphenylamine)-9,9′-spirobifluorene
(spiro-OMeTAD) (≥99.8%) was purchased from Borun New Material
Technology Co., Ltd.
tin chloride (SnCl2·2H2O) dehydrate, Al2O3 (aluminum oxide) NPs with <50 nm particle
size, magnesium acetate tetrahydrate [(CH3COO)2Mg·4H2O], lead(II) bromide (PbBr2), cesium iodide (CsI), tert-butylpyridine (TBP), Li bis(trifluoromethanesulfonyl)imide
(Li-TFSI), potassium hydroxide solution (KOH), and solvents dimethyl
sulfoxide (DMSO anhydrous, ≥99.9%), N,N-dimethylformamide (DMF anhydrous, 99.8%), diethyl ether
(99.0%), ethanol (99.8%), and 2-propanol anhydrous 99.5% were purchased
from Sigma-Aldrich. Tin(IV) oxide 15% in H2O colloidal
dispersion and 1-benzyl-3-methylimidazolium chloride (IL1) were purchased
from Alpha Aesar. 1-Butyl-3-methylimidazolium tetrafluoroborate (IL2)
was purchased from Acros Organics. Lead(II) iodide (PbI2) (99.99%, trace metal basis) was purchased from TCI Deutschland
GmbH. Formamidinium iodide (FAI) methylammonium bromide (MABr), and
methylammonium iodide (MAI) were purchased from GreatCell Solar. 18NR-T
titania paste and cobalt salt (III) FK209 TFSI (98%) were purchased
from Dyesol Limited. 2,2′,7,7′-Tetrakis-(N,N-di-p-methoxyphenylamine)-9,9′-spirobifluorene
(spiro-OMeTAD) (≥99.8%) was purchased from Borun New Material
Technology Co., Ltd.
6
Synthesis of Semiconductor Nanocrystals
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Cadmium oxide (CdO, 99.99%, trace metals), trioctylphosphine (TOP, 90% technical grade), sulfur (S, 99.98%), 1-octadecene (ODE, 90%, technical grade), iso-Octane (99.7%, HPLC grade), 1-Octanethiol (> 98.5%), trimethylammonium chloride (TMACl, > 98%), potassium hydroxide (KOH, 99.99%), dimethyl sulfoxide (DMSO, > 99.9%) magnesium acetate tetrahydrate (99%), zinc acetate dihydrate (> 98%), lithium acetate (99.95%) and 1-butanol (anhydrous, 99.8%) were purchased from Sigma-Aldrich. Zinc acetate anhydrous (+ 99.9%) and ethyl acetate (> 99.5%, ACS certified) were purchased from Thermoscientific. Selenium (Se, 99.999%, metals basis) and oleic acid (90%, technical grade) were purchased from Alfa-Aesar. Octane (+ 99%, extra pure) was purchased from Acros Organics. All the reagents were used as received. Poly(ethylene dioxythiophene): polystyrene sulfonate (PEDOT: PSS) was purchased from Ossila. Poly[(9,9-dioctylfluorenyl-2,7-diyl)-co-(4,4’-(N-(p-butylphenyl)) diphenylamine)] (TFB) was purchased from American Dye Source. Polyvinylpyrrolidone (PVP10) with an average molecular weight of 10,000 was purchased from Sigma-Aldrich. Patterned ITO-glass substrates with 15 Ω resistance and 25.4 mm × 25.4 mm × 0.7 mm were purchased from Luminescence Technology Corp.
7
DNA Origami Triangles and Nanotubes
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Silicon wafers [Si(110), with native oxide] and M13mp18 scaffold strands for DNA origami triangles were purchased from University Wafers (South Boston, MA, USA) and Bayou Biolabs (Metairie, LA, USA), respectively. Staple strands for the DNA origami triangles and strands for DNA nanotubes were synthesized by Integrated DNA Technologies (Coralville, IA, USA). 2-Amino-2-(hydroxymethyl)-1,3-propanediol (Tris), ethylenediaminetetraacetic acid (EDTA), magnesium acetate tetrahydrate, sulfuric acid, hydrogen peroxide solution (30% H2O2), and poly(L-lactide) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Acetic acid (glacial), dichloromethane, and ethanol were purchased from Fisher Scientific (Fair Lawn, NJ, USA), Acros Organics (Fair Lawn, NJ, USA), and Decon Laboratories, Inc. (King of Prussia, PA, USA), respectively. PDMS backing stamp was fabricated with Sylgard 184 silicone elastomer kit (Dow Corning, Midland, MI, USA). All materials were used as received. High-purity water (18.3 MΩ) was used throughout the entire experiment by using a Barnstead MicroPure Standard water purification system (Thermo Scientific, Waltham, MA, USA).
8
DNA Oligonucleotide Preparation and Characterization
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All DNA oligonucleotides used in this study were purchased from Integrated DNA Technologies (IDT) at the desalted grade. The actual sequences used are shown in Supplementary Table S1 . The lyophilized DNA was reconstituted in 1× Tris–EDTA buffer (1× TE, pH 8.0) to give 100 μM stock and stored at 4°C. The following chemicals were used as received: magnesium acetate tetrahydrate ((CH3COO)2Mg·4H2O, ≥99.0%), silver nitrate (AgNO3, ≥99.0%), sodium borohydride (NaBH4, 99%) were purchased from Sigma Aldrich. 0.1 M phosphate buffer (pH 7.4) was prepared using sodium phosphate dibasic, anhydrous (Na2HPO4, ≥99.0%) from Fischer Scientific and sodium phosphate monobasic dehydrate (NaH2PO4·2H2O, ≥99%) from Kanto Chemical Co. Inc. Milli-Q water (UP) with resistance >18.2 MΩ/cm was used throughout the experiment.
9
Hydrogel and DNA Functionalization Protocol
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Acrylamide (Bio-Rad, Cat. No. 161-0100) was solubilized using MilliQ purified water. Rhodamine B-conjugated methacrylate monomer was obtained from PolySciences, Inc (Cat. No. 25404-100) and used for fluorescent visualization of hydrogels. Hydrogels were polymerized using the photoactive initiator Irgacure 2100 (BASF). ATP was purchased from Sigma (Cat. No. A6419) and solubilized to 53 mM using MilliQ purified water. Unmodified and acrydite-modified DNA strands were purchased with standard desalting purification from Integrated DNA Technologies, Inc. Fluorophore- and quencher-modified DNA was purchased with HPLC purification. All DNA was solubilized using TAE buffer (Life Technologies, Cat. No. 24710-030) supplemented with 12.5 mM magnesium acetate tetrahydrate (Sigma, Cat. No. M5661). As described in Supplementary Figs. 2 , 9 , 18 , and 22 , DNA sequences were designed using NUPACK (Supplementary Methods )53 (link) or adapted from previous literature31 (link),37 (link),38 (link),54 (link). Sequences used in this study are found in Supplementary Table 1 .
10
Colorimetric Heavy Metal Detection
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Magnesium acetate tetrahydrate
[Mg(COOCH3)2·4H2O] (99%), HCl,
NaOH, HgCl2, and NaCl were purchased from Sigma-Aldrich.l -lysine was obtained from Fluka. For the strip preparation,
Whatman filter paper (Grade-1) was purchased from Sigma-Aldrich. All
chemicals were of analytical grade unless specified otherwise and
were used as such. Clove buds and V. radiata seeds were purchased from local market (Sector 32, Chandigarh).
DDW was used in all the experiments.
[Mg(COOCH3)2·4H2O] (99%), HCl,
NaOH, HgCl2, and NaCl were purchased from Sigma-Aldrich.
Whatman filter paper (Grade-1) was purchased from Sigma-Aldrich. All
chemicals were of analytical grade unless specified otherwise and
were used as such. Clove buds and V. radiata seeds were purchased from local market (Sector 32, Chandigarh).
DDW was used in all the experiments.
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