Soluble s cd14

Manufactured by R&D Systems

Sourced in Germany

Soluble (s)CD14 is a protein that functions as a pattern recognition receptor. It binds to lipopolysaccharide (LPS) and facilitates its interaction with the CD14/TLR4/MD-2 receptor complex.

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Lab products found in correlation

Hek blue htlr4, by InvivoGen (4 mentions) Hek blue hnod2, by InvivoGen (4 mentions) Hek blue htlr9, by InvivoGen (3 mentions) Interleukin 6 (il 6), by Thermo Fisher Scientific (1 mentions) Il 10, by Thermo Fisher Scientific (1 mentions) Immage analyzer, by Beckman Coulter (1 mentions) Vidas analyzer, by bioMérieux (1 mentions) Cytometric bead array system, by BD (1 mentions) Resistin, by R&D Systems (1 mentions)

5 protocols using soluble s cd14

Biomarker Analysis in Fasting Participants

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Individuals had been fasting (12 hours) before their visit. Blood samples were retrieved and processed at a single center and stored at –80°C until use. Interleukin (IL)-6, IL-10 (Bender Medsystems, Burlingame, CA, USA), resistin, and soluble(s)CD14 (R&D Systems, Minneapolis, MN, USA) levels were analyzed using an enzyme-linked immunosorbent assay. Serum ultrasensitive C-reactive protein (us-CRP) was measured by immononephelometry on an IMMAGE analyzer (Beckman-Coulter, Miami, FL, USA). Plasma D-dimer was measured by enzyme-linked fluorescent assay on a VIDAS analyzer (Biomérieux, Marcy-l’Etoile, France). Inducible protein (IP)–10, fractalkine, Monokine induced by gamma interferon (MIG), monocyte chemoattractant protein (MCP)–1, E-selectin, and tumor necrosis factor (TNF)–α were quantified from plasma using the BD Cytometric Bead Array system (BD, Franklin Lakes, NJ, USA).

Boyd A., Boccara F., Meynard J.L., Ichou F., Bastard J.P., Fellahi S., Samri A., Sauce D., Haddour N., Autran B., Cohen A., Girard P.M, & Capeau J. (2019). Serum Tryptophan-Derived Quinolinate and Indole-3-Acetate Are Associated With Carotid Intima-Media Thickness and its Evolution in HIV-Infected Treated Adults. Open Forum Infectious Diseases, 6(12), ofz516.

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Measuring Gut Inflammation Biomarkers

Cited 1 time

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Enzyme linked immunosorbent assays (ELISA) were used to measure fecal and serum calprotectin, fecal and serum zonulin, serum diamine oxidase (Immundiagnostic AG, Bensheim, Germany), soluble (s)CD14 (R&D Systems, Minneapolis, USA), and lipopolysaccharide binding protein (LBP) (Hycult biotech, Uden, The Netherlands). All assays were performed according to manufacturers’ instructions. Bacterial products (endotoxin, peptidoglycan and bacterial DNA) were detected in serum using HEK-Blue hTLR4, HEK-Blue hNOD2 and HEK-Blue hTLR9 reporter cells (Invivogen, Toulouse, France), respectively as published previously [23 (link)].

Stadlbauer V., Engertsberger L., Komarova I., Feldbacher N., Leber B., Pichler G., Fink N., Scarpatetti M., Schippinger W., Schmidt R, & Horvath A. (2020). Dysbiosis, gut barrier dysfunction and inflammation in dementia: a pilot study. BMC Geriatrics, 20, 248.

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Gut Barrier and Microbial Products Assessment

Cited 1 time

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Enzyme linked immunosorbent assays (ELISA) were used to measure fecal and serum calprotectin, fecal and serum zonulin, serum diamine oxidase (Immundiagnostik AG, Bensheim, Germany), soluble (s)CD14 (R&D Systems, Minneapolis, USA), and lipopolysaccharide binding protein (LBP) (Hycult Biotech, Uden, The Netherlands). All assays were performed according to manufacturers’ instructions.
For the lactulose/mannitol/sucrose test, after overnight fasting, the study participants drank a solution of 100 ml water containing 10g lactulose, 5g D-mannitol and 20g sucrose. Urine is collected over 5 hours. After 2 hours consumption of water is allowed. Sugar recovery in urine test was analyzed by NMR spectroscopy as published previously [12 (link)].
Bacterial products (endotoxin, peptidoglycan and bacterial DNA) were detected in serum using HEK-Blue hTLR4, HEK-Blue hNOD2 and HEK-Blue hTLR9 reporter cells (Invivogen, Toulouse, France), respectively as published previously [12 (link)].

Stadlbauer V., Horvath A., Komarova I., Schmerboeck B., Feldbacher N., Wurm S., Klymiuk I., Durdevic M., Rainer F., Blesl A., Stryeck S., Madl T., Stiegler P, & Leber B. (2019). A single alcohol binge impacts on neutrophil function without changes in gut barrier function and gut microbiome composition in healthy volunteers. PLoS ONE, 14(2), e0211703.

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Intestinal Barrier Dysfunction Biomarkers

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Enzyme linked immunosorbent assays (ELISA) were used to measure fecal and serum calprotectin, fecal and serum zonulin, serum diamine oxidase (Immundiagnostic AG, Bensheim, Germany), soluble (s)CD14 (R&D Systems, Minneapolis, USA), and lipopolysaccharide binding protein (LBP) (Hycult biotech, Uden, The Netherlands). All assays were performed according to manufacturers' instructions. Bacterial products (endotoxin, peptidoglycan and bacterial DNA) were detected in serum using HEK-Blue hTLR4, HEK-Blue hNOD2 and HEK-Blue hTLR9 reporter cells (Invivogen, Toulouse, France), respectively as published previously [23] .

Stadlbauer V., Engertsberger L., Komarova I., Feldbacher N., Leber B., Pichler G., Fink N., Scarpatetti M., Schippinger W., Schmidt R., & Horvath A. (2020). Dysbiosis, gut barrier dysfunction and inflammation in dementia: A pilot study.

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Intestinal Barrier Dysfunction Biomarkers

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Enzyme linked immunosorbent assays (ELISA) were used to measure fecal and serum calprotectin, fecal and serum zonulin, serum diamine oxidase (Immundiagnostic AG, Bensheim, Germany), soluble (s)CD14 (R&D Systems, Minneapolis, USA), and lipopolysaccharide binding protein (LBP) (Hycult biotech, Uden, The Netherlands). All assays were performed according to manufacturers' instructions. Bacterial products (endotoxin, peptidoglycan and bacterial DNA) were detected in serum using HEK-Blue hTLR4, HEK-Blue hNOD2 and HEK-Blue hTLR9 reporter cells (Invivogen, Toulouse, France), respectively as published previously [23] .

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