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Csu x1a 5000

Manufactured by Yokogawa
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The CSU-X1A 5000 is a confocal scanning unit that provides high-speed and high-resolution imaging for fluorescence microscopy applications. It features a spinning disk design and supports a wide range of fluorescence dyes and proteins.

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Lab products found in correlation

Neomycin, by Merck Group (2 mentions) Cell tak, by BD (2 mentions) Volocity software, by PerkinElmer (2 mentions) Metamorph nx 2.0, by Olympus (2 mentions) 4 oh tamoxifen, by Merck Group (2 mentions) 35 mm glass bottom dishes, by BD (2 mentions) Plan neofluar, by Zeiss (1 mentions) Axio observer z1, by Zeiss (1 mentions) Fibronectin, by MatTek (1 mentions)

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CSU-X1A 5000 unit (2 citations)

4 protocols using csu x1a 5000

1

High-resolution Spinning Disk Confocal Imaging

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Imaging was performed using an inverted Zeiss Axio Observer.Z1 research microscope (Carl Zeiss Microscopy GmbH, Jena, Germany) equipped with a Yokogawa CSU-X1A 5000 spinning disk confocal scanning unit (Yokogawa Denki, Musashino, Japan). This setup enables high-resolution spinning disk confocal microscopy with up to four different wavelengths.
The stained tissue samples were excited with lasers corresponding to their respective stains, namely 488 nm (Col-F), 561 nm (SPY555-DNA), and 638 nm (SiR-actin). They were then imaged for different magnifications and to a depth of about 50 µm from their surface. Subsequently, the individual channels were slightly adjusted in terms of contrast and superimposed.
Eichholz H.M., Cornelis A., Wolf B., Grubitzsch H., Friedrich P., Makky A., Aktas B., Käs J.A, & Stepan H. (2023). Anatomy of the fetal membranes: insights from spinning disk confocal microscopy. Archives of Gynecology and Obstetrics, 309(5), 1919-1923.
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2

Imaging Hair Cell Regeneration

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Utricles harvested from P3-P5 Lgr5EGFP-CreERT2/+; Rosa26RtdTomato/+ mice were attached to 35 mm glass bottom dishes (MatTek) pre-coated with CellTaK (BD Biosciences). Whole organs were cultured overnight, then treated with neomycin (1.0 mM × 24hr, Sigma) as above. 4OH-tamoxifen (500 nM, Sigma) in growth factor-enriched, serum-free media was present for 2–4 days first. Then organs were imaged in DMEF/F12 without phenol red (1:1 Cellgro) media using a spinning disc confocal imaging system (Zeiss Axio Observer Z1 or OlympusIX-81 coupled with a Yokogawa spinning disc system CSU-X1A 5000) connected to an incubating chamber (37°C, 5% CO2). One to two EGFP+ regions with tdTomato+ cells were selected from each utricle, and z-stack images spanning the sensory epithelium were taken at 0.5–1 hr intervals. Collected videos were processed and analyzed with ImageJ64 (NIH), MetaMorph (NX 2.0; Olympus) and Volocity software (v6.1.0; Improvision).
Wang T., Chai R., Kim G.S., Pham N., Jansson L., Nguyen D.H., Kuo B., May L., Zuo J., Cunningham L.L, & Cheng A.G. (2015). Lgr5+ Cells Regenerate Hair Cells via Proliferation and Direct Transdifferentiation in Damaged Neonatal Mouse Utricle. Nature communications, 6, 6613.
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3

Imaging Hair Cell Regeneration

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Utricles harvested from P3-P5 Lgr5EGFP-CreERT2/+; Rosa26RtdTomato/+ mice were attached to 35 mm glass bottom dishes (MatTek) pre-coated with CellTaK (BD Biosciences). Whole organs were cultured overnight, then treated with neomycin (1.0 mM × 24hr, Sigma) as above. 4OH-tamoxifen (500 nM, Sigma) in growth factor-enriched, serum-free media was present for 2–4 days first. Then organs were imaged in DMEF/F12 without phenol red (1:1 Cellgro) media using a spinning disc confocal imaging system (Zeiss Axio Observer Z1 or OlympusIX-81 coupled with a Yokogawa spinning disc system CSU-X1A 5000) connected to an incubating chamber (37°C, 5% CO2). One to two EGFP+ regions with tdTomato+ cells were selected from each utricle, and z-stack images spanning the sensory epithelium were taken at 0.5–1 hr intervals. Collected videos were processed and analyzed with ImageJ64 (NIH), MetaMorph (NX 2.0; Olympus) and Volocity software (v6.1.0; Improvision).
Wang T., Chai R., Kim G.S., Pham N., Jansson L., Nguyen D.H., Kuo B., May L., Zuo J., Cunningham L.L, & Cheng A.G. (2015). Lgr5+ Cells Regenerate Hair Cells via Proliferation and Direct Transdifferentiation in Damaged Neonatal Mouse Utricle. Nature communications, 6, 6613.
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4

Spinning Disk Confocal Microscopy

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Cells were imaged using a spinning disk confocal microscope (ZEISS) equipped with a CSU-X1A 5000 spinning disk unit (Yokogawa Electric Corp.), multilaser module with wavelengths of 458, 488, 514, and 561 nm, and an Axio Observer Z1 motorized inverted microscope equipped with a precision motorized XY stage (ZEISS). Images were acquired with a Zeiss Plan-Neofluar 40× 1.3-NA or 64× 1.4-NA objective on an Orca R2 CCD camera using Zen 2012 software (ZEISS). Cells were plated on dishes containing coverslips coated with fibronectin (MatTek Corporation) 24 h before treatment or transfection.
Ando K., Parsons M.J., Shah R.B., Charendoff C.I., Paris S.L., Liu P.H., Fassio S.R., Rohrman B.A., Thompson R., Oberst A., Sidi S, & Bouchier-Hayes L. (2017). NPM1 directs PIDDosome-dependent caspase-2 activation in the nucleolus. The Journal of Cell Biology, 216(6), 1795-1810.
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