Western blots were carried out as described previously (Ventoso et al., 2006 (link)) using the following primary antibodies: anti-RPS4X (sc-85133, Santa Cruz Biotech.), anti-RPS7 (sc-377317, Santa Cruz Biotech.), anti-RPS6 (sc-74576, Santa Cruz Biotech.), anti-eIF4A (STJ2724, St. John´s lab,), anti-eIF3g (STJ23512, St. John´s lab), anti-EGFP (11814460001, Roche), anti-eEF1A1 (2551, Cell Signaling), anti-AKT1 (9272, Cell Signaling), anti-ACTB (T-5168, Sigma), anti-eIF4b (sc-376062, Santa Cruz Biotech.), anti-HRas (sc-53959, Santa Cruz Biotech.), anti-CCND3 (sc-453, Santa Cruz Biotech.), anti-ODC1 (sc-398116, Santa Cruz Biotech.), anti-GRK2 (a gift from C. Murga, CBMSO). Blots were developed with ECL (GE) and bands were quantified by densitometry. Immunofluorescence analysis was carried out as described previously using anti-RPS7 (1:500) and anti-mouse Alexa 595 as secondary antibody (Invitrogen). The preparations were analyzed under a Nikon A1R confocal microscope.
Anti ccnd3
Manufactured by Santa Cruz Biotechnology
Anti-CCND3 is a lab equipment product that targets the CCND3 protein. CCND3 is a member of the cyclin family and plays a role in cell cycle regulation. The Anti-CCND3 product can be used for research purposes, but no further details on its intended use or application are provided.
Automatically generated - may contain errors
Lab products found in correlation
Anti akt1, by Cell Signaling Technology (1 mentions)
Anti rps6, by Santa Cruz Biotechnology (1 mentions)
Anti egfp, by Roche (1 mentions)
Anti actb, by Merck Group (1 mentions)
Anti hras, by Santa Cruz Biotechnology (1 mentions)
Anti eef1a1, by Cell Signaling Technology (1 mentions)
Anti eif4b, by Santa Cruz Biotechnology (1 mentions)
Enhanced chemi luminescence (ecl), by GE Healthcare (1 mentions)
A1r confocal microscope, by Nikon (1 mentions)
Anti ccnd1, by Cell Signaling Technology (1 mentions)
Ecl plus detection kit, by GE Healthcare (1 mentions)
Protease inhibitor, by Thermo Fisher Scientific (1 mentions)
Radioimmunoprecipitation assay (ripa), by Thermo Fisher Scientific (1 mentions)
Anti rabbit, by Vector Laboratories (1 mentions)
Anti tubulin, by Thermo Fisher Scientific (1 mentions)
Anti mouse, by Vector Laboratories (1 mentions)
Anti goat, by Santa Cruz Biotechnology (1 mentions)
Anti gapdh, by Cell Signaling Technology (1 mentions)
2 protocols using anti ccnd3
1
Western Blotting and Immunofluorescence Analysis
2
Western Blot Profiling of Oncogenic Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were washed 3 times with PBS and lysed on ice with RIPA (Thermo scientific, 89900) supplemented with EDTA and protease inhibitors (Thermo Scientific, 78410). Equal amounts of protein were loaded and separated on an SDS-PAGE gel of 6, 8 or 12%. Proteins were transferred onto nitrocellulose 0.2 μm membrane (Biorad) using wet transfer (Tris/glycine, 20% ethanol) overnight (15h) at 30V. Detection was performed with ECL Plus detection kit (GE-Healthcare) using either the digital camera (GE Healthcare, LAS 4000) or the Agfa Gevaert, CURIX 60 system.
Primary antibodies for detection were as follows: anti-SASH1 (Novus, NBP1-26650), anti-CCND3 (Santa Cruz Biotechnologies Sc-182), anti-CCND1 (Cell Signaling, # 2926), anti-GAPDH (Cell Signaling #21185), anti-tubulin (Invitrogen, 322500). The following secondary antibodies: anti-goat (Santa Cruz Biotechnology, Sc-2020), anti-rabbit (Vector laboratories, PI1000) and anti mouse (Vector Laboratories, PI2000) coupled with horseradish peroxidase were used for detection.
Primary antibodies for detection were as follows: anti-SASH1 (Novus, NBP1-26650), anti-CCND3 (Santa Cruz Biotechnologies Sc-182), anti-CCND1 (Cell Signaling, # 2926), anti-GAPDH (Cell Signaling #21185), anti-tubulin (Invitrogen, 322500). The following secondary antibodies: anti-goat (Santa Cruz Biotechnology, Sc-2020), anti-rabbit (Vector laboratories, PI1000) and anti mouse (Vector Laboratories, PI2000) coupled with horseradish peroxidase were used for detection.
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