Specimens that had been fixed and stored in 70% ethanol were processed for SEM following standard methods [29 ]. These included critical point drying (CPD) in sample baskets and mounting on SEM sample mounts (stubs) using conductive double-sided carbon tape. Samples were coated with gold and palladium for 3 min using a Polaron #3500 sputter coater (Quorum (Q150 TES) https://www.quorumtech.com ) establishing an approximate thickness of 20 nm. Samples were placed and observed in an FEI Helios Dual Beam Nanolab 600 (FEI, Hillsboro, Oregon) Scanning Electron Microscope, with digital images obtained in the Nanolab software system (FEI, Hillsboro, Oregon), and then transferred to a USB for future reference. Samples were received under low vacuum conditions using 10 kV, spot size 2, 0.7 Torr using a GSE detector.
Nanolab software system
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Nanolab software system is a comprehensive platform designed for analyzing and managing data from nanomaterials research and development. It provides a suite of tools for data acquisition, analysis, and reporting, facilitating efficient and streamlined workflows in nanomaterials laboratories.
Automatically generated - may contain errors
10 protocols using nanolab software system
1
Preparing Biological Samples for SEM Imaging
2
SEM Imaging of Ethanol-Fixed Specimens
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Two specimens that had been fixed and stored in 70% ethanol were processed for SEM following standard methods [27 ]. These included critical point drying (CPD) in sample baskets and mounting on SEM sample mounts (stubs) using conductive double-sided carbon tape. Samples were coated with gold and palladium for 3 min using a Polaron #3500 sputter coater (Quorum (Q150 TES) https://www.quorumtech.com ), establishing an approximate thickness of 20 nm. Samples were placed and observed in an FEI Helios Dual Beam Nanolab 600 (FEI, Hillsboro, Oregon, USA) Scanning Electron Microscope with digital images obtained in the Nanolab software system (FEI) and then transferred to a USB for future reference. Samples were received under low vacuum conditions using 10 kV, spot size 2, 0.7 Torr using a GSE detector.
3
Scanning Electron Microscopy of Preserved Parasites
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Samples of parasites that had been fixed and stored in 70% ethanol were processed following standard methods [18 ]. These included critical point drying (CPD) in sample baskets and mounting on SEM sample mounts (stubs) using conductive double-sided carbon tape. Samples were coated with gold and palladium for 3 minutes using a Polaron #3500 sputter coater (Quorum (Q150 TES) www.quorumtech.com ), establishing an approximate thickness of 20 nm. Samples were placed and observed in an FEI Helios Dual-Beam Nanolab 600 Scanning Electron Microscope (FEI, Hillsboro, OR, USA) with digital images obtained in the Nanolab software system (FEI, Hillsboro, OR, USA), and then transferred to a USB key for future reference. Images were taken at various magnifications. Samples were received under low vacuum conditions using 10 KV, spot size 2, 0.7 Torr using a GSE detector.
4
Preparation and Imaging of Biological Specimens for SEM
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About 15 specimens from four host species that had been fixed and stored in 70% ethanol were processed for SEM following standard methods [36 ]. These included critical point drying (CPD) in sample baskets and mounting on SEM sample mounts (stubs) using conductive double sided carbon tape. Samples were coated with gold and palladium for 3 min using a Polaron #3500 sputter coater (Quorum (Q150 TES) www.quorumtech.com ) establishing an approximate thickness of 20 nm. Samples were placed and observed in an FEI Helios Dual Beam Nanolab 600 (FEI, Hillsboro, Oregon) Scanning Electron Microscope, with digital images obtained in the Nanolab software system (FEI, Hillsboro, Oregon) and then stored on a USB for future reference. Samples were received under low vacuum conditions using 10 kV, spot size 2, 0.7 Torr using a GSE detector.
5
Scanning Electron Microscopy of Fixed Parasites
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Samples of parasites that had been fixed and stored in 70% ethanol were processed following standard methods. These included critical point drying (CPD) in sample baskets and mounting on SEM sample mounts (stubs) using conductive double-sided carbon tape. Samples were coated with gold and palladium for 3 minutes using a Polaron #3500 sputter coater (Quorum (Q150 TES) www.quorumtech.com ) establishing an approximate thickness of 20 nm. Samples were placed and observed in an FEI Helios Dual Beam Nanolab 600 (FEI, Hillsboro, Oregon). Scanning Electron Microscope with digital images were obtained in the Nano lab software system (FEI, Hillsboro, Oregon). Images were taken at various magnifications. Samples were received under low vacuum conditions using 10 KV, spot size 2, 0.7 Torr using a GSE detector.
6
Specimen Preparation for SEM Imaging
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Four to six specimens that had been fixed and stored in 70% ethanol were processed for SEM following standard methods [23 ]. These included critical point drying (CPD) in sample baskets and mounting on SEM sample mounts (stubs) using conductive double-sided carbon tape. Samples were coated with gold and palladium for 3 min using a Polaron #3500 sputter coater (Quorum (Q150 TES) www.quorumtech.com ) establishing an approximate thickness of 20 nm. Samples were placed and observed in an FEI Helios Dual Beam Nanolab 600 (FEI, Hillsboro, Oregon, USA) Scanning Electron Microscope with digital images obtained in the Nanolab software system (FEI, Hillsboro, Oregon, USA) and then stored on a USB for future reference. Samples were received under low vacuum conditions using 10 KV, spot size 2, 0.7 Torr using a GSE detector.
7
Specimen Preparation for SEM Imaging
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Specimens that had been fixed and stored in 70% ethanol were processed for scanning electron microscopy (SEM) following standard methods [10 ]. These included critical point drying (CPD) in sample baskets and mounting on SEM sample mounts (stubs) using conductive double-sided carbon tape. Samples were coated with gold and palladium for 3 min using a Polaron #3500 sputter coater (Quorum (Q150 TES; www.quorumtech.com ) establishing an approximate thickness of 20 nm. Samples were placed and observed in an FEI Helios Dual Beam Nanolab 600 (FEI, Hillsboro. Oregon) scanning electron microscope with digital images obtained in the Nanolab software system (FEI, Hillsboro, OR) and then transferred to a USB for future reference. Samples were received under low vacuum conditions using 10 KV, spot size 2, 0.7 Torr, using a GSE detector.
8
SEM Imaging of Parasite Samples
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Samples of parasite that had been fixed and stored in 70% ethanol were processed following standard methods [21 ]. These included critical point drying (CPD) in sample baskets and mounting on SEM sample mounts (stubs) using conductive double-sided carbon tape. Samples were coated with gold and palladium for 3 min using a Polaron #3500 sputter coater (Quorum (Q150 TES) www.quorumtech.com ) establishing an approximate thickness of 20 nm. Samples were placed and observed in an FEI Helios 660 NanoLab DualBeam (FEI, Hillsboro, OR) scanning electron microscope, with digital images obtained in the Nanolab software system (FEI, Hillsboro, OR), and then transferred to a USB for future reference. Images were taken at various magnifications. Samples were received under low vacuum conditions using 10 KV, spot size 2, 0.7 Torr using a GSE detector.
9
Scanning Electron Microscopy of Parasites
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Samples of parasites that had been fixed and stored in 70% ethanol were processed following standard methods [18 ] which included critical point drying (CPD) in sample baskets and mounted on SEM sample mounts (stubs) using conductive double-sided carbon tape. Samples were coated with gold and palladium for 3 min using a Polaron #3500 sputter coater (Quorum (Q150 TES) www.quorumtech.com ) establishing an approximate thickness of 20 nm. Samples were placed and observed in an FEI Helios Dual Beam Nanolab 600 (FEI, Hillsboro, OR, USA) Scanning Electron Microscope with digital images obtained in the Nanolab software system (FEI) and then transferred to a USB for future reference. Images were taken at various magnifications. Samples were received under low vacuum conditions using a 10 kV, spot size 2 0.7 Torr using a GSE detector.
10
SEM Preparation of Ethanol-Fixed Specimens
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Specimens that had been fixed and stored in 70% ethanol were processed for SEM following standard methods (Lee, 1992) . These included critical-point drying in sample baskets and mounting on SEM sample mounts (stubs) using conductive double-sided carbon tape. Samples were coated with gold and palladium for 3 min using a Polaron #3500 sputter coater (Q150 TES, Quorum: www.quorumtech.com) establishing an approximate thickness of 20 nm. Samples were placed and observed in an FEI Helios Dual Beam Nanolab 600 (FEI, Hillsboro, Oregon, USA) scanning electron microscope, with digital images obtained in the Nanolab software system (FEI, Hillsboro, Oregon, USA) and then transferred to a USB for future reference. Samples were received under low vacuum conditions using 10 KV, spot size 2, 0.7 torr using a gaseous secondary electron detector.
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