Meso scale discovery (msd)

Manufactured by MSD

Sourced in United States

Meso Scale Discovery is a versatile laboratory equipment used for various scientific applications. It employs electrochemiluminescence technology to enable sensitive and reliable detection and quantification of a wide range of analytes, including proteins, peptides, and small molecules. The system provides a platform for performing multiplex assays, allowing for the simultaneous measurement of multiple targets in a single sample.

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Lab products found in correlation

Sector imager 2400, by MSD (3 mentions) V plex aβ peptide panel 6e10 kit, by MSD (2 mentions) Bicinchoninic acid assay, by Thermo Fisher Scientific (2 mentions) Discovery workbench software, by Mesoscale (1 mentions) Msd sector imager 2400, by MSD (1 mentions) Odyssey fc system, by LI COR (1 mentions) Image studio, by LI COR (1 mentions) V plex plus kit, by MSD (1 mentions) Multiplex bead assay, by Bio-Rad (1 mentions) R d quantikine elisa kit, by R&D Systems (1 mentions) Meso scale discovery (msd), by Mesoscale (1 mentions) Phosphate inhibitor cocktail 3, by Merck Group (1 mentions) Pierce bsa protein assay kit, by Thermo Fisher Scientific (1 mentions) Phosphate inhibitor cocktail 2, by Merck Group (1 mentions) Tissuelyzer 2, by Qiagen (1 mentions) Complete mini, by Roche (1 mentions) Msd discovery workbench 4, by Mesoscale (1 mentions) Quickplex sq 120, by MSD (1 mentions) Meso scale discovery 6000, by Mesoscale (1 mentions) Elisa max standard set human il 6, by BioLegend (1 mentions)

Spelling variants of this product

Meso Scale Discovery (MSD; (3 citations) Meso Scale DiscoveryTM; (2 citations)

59 protocols using meso scale discovery (msd)

Measuring IL15 Shedding from Cells

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Example 80

To test if IL15 is shed into the media, supernatant from HCT116 cells expressing IL15-IL15Ra fusion constructs was subject to immunoassays such as ELISA and MSD (Rockville, Md.). In FIG. 54B, OD indicates optical density. IL15 was measured in the media of cells expressing IL15-IL15Ra fusion constructs. OT-IL15-009 construct demonstrated a TMP dose dependent increase in L 5 levels detected in the media. The level of IL15 in the media detected with the constitutive construct OT-IL15-008 was much higher than the levels detected with the DD regulated constructs (FIG. 54B). As shown in FIG. 54C, a dose dependent increase in L 5 levels was observed with both the DD regulated constructs using the MSD immunoassay. The detection of membrane bound IL15-IL15Ra fusion constructs in the supernatant suggests that IL15 constructs are likely shed from the cell surface.

US11629340B2. DHFR tunable protein regulation (2023-04-18). OBSIDIAN THERAPEUTICS, INC. [US]. Inventors: Vipin Suri [US], Dan Jun Li [US], Dexue Sun [US], Byron Delabarre [US], Vijaya Balakrishnan [US], Brian Dolinski [US], Mara Christine Inniss [US], Grace Y. Olinger [US].

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Correlation of Biomarkers in Primary HLH

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Example 2

The studies presented herein are from an ongoing phase 2 pilot study in primary HLH patients who were administered the NI-0501 antibody and from patients who received the NI-0501 antibody in compassionate use.

As shown in FIG. 1, serum levels of CXCL9 and IFNγ were measured by Luminex and Meso Scale Discovery (MSD) technology, respectively, in samples obtained from 6 primary HLH patients and from 3 compassionate use patients. Correlations were performed between CXCL9 and total IFNγ concentrations. Statistics were performed and p values were obtained using the Spearman test.

As shown in FIG. 2, predose serum levels of CXCL9 and IFNγ were measured by Luminex and MSD technology, respectively, in samples obtained from 6 primary HLH patients and 3 compassionate use patients. Correlations were performed between CXCL9 and total IFNγ concentrations. Statistics were performed and p values obtained using the Spearman test.

US11236158B2. Methods, compositions and dosing regimens for treating or preventing interferon-gamma related indications (2022-02-01). Swedish Orphan Biovitrum AG [CH]. Inventors: Cristina De Min [CH], Walter Ferlin [CH], Fabrizio De Benedetti [CH].

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Measuring Lower Limb and Hand Strength

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Maximum quadriceps strength was assessed using a hand-held dynamometer (microFET2, Biometrics, Almere, The Netherlands) in a sitting position with the knee flexed at 90°. Participants were instructed to stretch their knee as much as possible. After measuring the lever distance (knee joint—application), the sum of the maximum force momentum [Nm] from a single trial of each leg was used for analysis.
Maximum hand grip strength [kg] was assessed with a dynamometer (Jamar, MSD, Londerzeel, Belgium, respectively). The value of a single trial with the preferred hand was used for analysis.

Lindemann U., Mohr C., Machann J., Blatzonis K., Rapp K, & Becker C. (2016). Association between Thigh Muscle Volume and Leg Muscle Power in Older Women. PLoS ONE, 11(6), e0157885.

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Serum Biomarkers in Primary HLH

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Example 2

US11091543B2. Methods, compositions and dosing regimens for treating or preventing interferon-gamma related indications (2021-08-17). Swedish Orphan Biovitrum AG [CH]. Inventors: Cristina de Min [CH], Walter Ferlin [CH], Fabrizio De Benedetti [CH].

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Multiplex Cytokine Profiling Protocol

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Cytokines were measured using a multiplex panel (IFN-α2a, IFN-λ, IFN-γ, IL-6, IL-8, monocyte chemoattractant protein 1 [MCP-1], interferon-inducible T cell alpha chemoattractant [I-TAC], IL-15) (MSD, MesoScale Discovery, Gaithersburg, USA) assay. The MSD assay was performed following the manufacturer's instructions using an MSD Sector Imager 2400. Cytokine concentrations were calculated using the Discovery Workbench software (version 4.0, Mesoscale Discovery, Gaithersburg, MD, USA) and based on a 4-fold serial diluted standard. CXCL10/IP-10 was quantified using a commercially available ELISA (R&D Systems, Wiesbaden, Germany) according to the manufacturer's instruction.

Danov O., Wolff M., Bartel S., Böhlen S., Obernolte H., Wronski S., Jonigk D., Hammer B., Kovacevic D., Reuter S., Krauss-Etschmann S, & Sewald K. (2020). Cigarette Smoke Affects Dendritic Cell Populations, Epithelial Barrier Function, and the Immune Response to Viral Infection With H1N1. Frontiers in Medicine, 7, 571003.

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Quantification of GSK3β and MLC-2 Phosphorylation

Cited 1 time

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GSK3β assay was performed on platelet rich plasma (Banerji et al. 2018 (link)) using assays validated to Good Clinical Practice standards on the MesoScale Discovery (MSD®). To monitor the phosphorylation status of GSK3β the samples were added to the plate followed by a solution containing the MSD SULFO-TAGTM labelled detection antibody for GSK3β.The plate was then analysed on the MSD SECTORTM Imager 6000 (Yap et al. 2014 (link)). The levels of pSer9 GSK3β, represented as a percent of the pre-treatment levels, were normalised to the levels of total GSK3β.
Myosin regulatory light chain 2 (MLC-2) levels of phosphorylated Ser19 and total MLC-2 were measured in lysates of platelets, isolated from blood by centrifugation, by Western blotting using fluorescent tagged IgG secondary antibodies and GAPDH as a loading control. The assay was validated by the ICR Clinical PD Biomarker group for use in clinical trials including low, medium and high Quality Controls. Fluorescent detection by LI-COR Odyssey Fc system and densitometric analysis of the images was performed using Image Studio (LI-COR).

Pal A., Asad Y., Ruddle R., Henley A.T., Swales K., Decordova S., Eccles S.A., Collins I., Garrett M.D., De Bono J., Banerji U, & Raynaud F.I. (2020). Metabolomic changes of the multi (-AGC-) kinase inhibitor AT13148 in cells, mice and patients are associated with NOS regulation. Metabolomics, 16(4), 50.

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Measuring Cytokine Levels in Microglia

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Cytokine levels in the microglia cell culture media were measured with the Meso Scale Discovery (MSD, Kenilworth, NJ, USA) proinflammatory mouse V‐Plex Plus kit [21].

Jørgensen L.Ø., Hyrlov K.H., Elkjaer M.L., Weber A.B., Pedersen A.E., Svenningsen Å.F, & Illes Z. (2020). Cladribine modifies functional properties of microglia. Clinical and Experimental Immunology, 201(3), 328-340.

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Plasma Immunoglobulin Profiling in Mice

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Blood was taken by a puncture of the saphenous vein, heart puncture, or retro-orbital plexus (Li-heparin-coated tubes; KADE, Nümbrecht, Germany) and centrifuged to separate cells and plasma. Total plasma IgE was measured in AROM+ and WT male mice at different age points using a classical immunoassay isotope-specific sandwich ELISA (BD Pharmingen). The determination of the immunoglobulin isotype levels (IgG1, IgG2a, IgG3, IgM and IgA) was performed either with a multiplex bead assay (Biorad, CA, USA) or with a combined electrochemiluminescence multiplexed assay system (Meso Scale Discovery, MSD, Rockville, MD USA).

Aguilar-Pimentel J.A., Cho Y.L., Gerlini R., Calzada-Wack J., Wimmer M., Mayer-Kuckuk P., Adler T., Schmidt-Weber C.B., Busch D.H., Fuchs H., Gailus-Durner V., Ollert M., Hrabě de Angelis M., Ohlsson C., Poutanen M., Teperino R, & Strauss L. (2020). Increased estrogen to androgen ratio enhances immunoglobulin levels and impairs B cell function in male mice. Scientific Reports, 10, 18334.

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Taurolidine Infusion for Postoperative Outcomes

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A randomised, multicentre, placebo controlled, open label clinical trial was performed. Three centres recruited patients including Cork University Hospital, Bons Secours Cork and Mercy University Hospital. Patients were randomised on a 1:1 allocation ratio to 2% Taurolidine infusions or to a placebo, given 4 times a day for a total of 4 days. A sealed envelope method was used for randomisation. Randomisation codes were generated from www.randomization.com.
The investigational medicinal product was an intravenous formulation of 2% Taurolidine (C₇H₁₆N₄O₄S₂) manufactured by Geistlich-Pharma AG, CH 6110 Wolhusen/Luzern, Switzerland. The comparator placebo was 0.9% saline. The Taurolidine solution required central administration and all patients randomised to receive Taurolidine had either a central line or a peripheral long line inserted prior to the operation. First dose of Taurolidine or Placebo was administered at induction of anaesthesia. Trial bloods were performed pre-operatively, and at 3 h, 6 h, day 1, day 2, day3, day 5 and day 7 (only if still an inpatient) post-operatively. Human IFN-γ, IL-1β, IL-2, IL-6, IL-10, TNF-α, Human VEGF, Human CRP levels were measured using a customised ELISA kit manufactured by MSD (Meso Scale Discovery)® (Gaithersburg, Maryland, US).

Redmond H.P., Neary P.M., Jinih M., O’Connell E., Foley N., Pfirrmann R.W., Wang J.H, & O’Leary D.P. (2018). RandomiSed clinical trial assessing Use of an anti-inflammatoRy aGent in attenUating peri-operatiVe inflAmmatioN in non-meTastatic colon cancer – the S.U.R.G.U.V.A.N.T. trial. BMC Cancer, 18, 794.

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Quantification of Cytokine Levels

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The concentrations of IL-1β, IL-10, TNF, IL-6, IL-8, IL-12p70 and IFN-γ present in the supernatants were quantified using Meso Scale Discovery (MSD, Rockville, MD), according to the manufacturer’s protocol.

Ó Maoldomhnaigh C., Cox D.J., Phelan J.J., Malone F.D., Keane J, & Basdeo S.A. (2021). The Warburg Effect Occurs Rapidly in Stimulated Human Adult but Not Umbilical Cord Blood Derived Macrophages. Frontiers in Immunology, 12, 657261.

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