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Bio plex platform

Manufactured by Bio-Rad
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The Bio-Plex platform is a multiplex assay system that allows for the simultaneous detection and quantification of multiple analytes in a single sample. The platform utilizes fluorescently-labeled magnetic beads and a dedicated flow cytometer to enable high-throughput analysis of protein, nucleic acid, and other biomolecular targets.

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Lab products found in correlation

Human immune monitoring 65 plex procartaplex immunoassay kit, by Thermo Fisher Scientific (4 mentions) Milliplex kit, by Merck Group (3 mentions) Bio plex pro human cytokine 27 plex immunoassay, by Bio-Rad (2 mentions) Milliplex human cytokine panel a immunoassay, by Merck Group (2 mentions) Ab300071, by Abcam (1 mentions) Ve cadherin, by Thermo Fisher Scientific (1 mentions) Eclipse te300 fluorescent microscope, by Nikon (1 mentions) Alexa flour 555, by Thermo Fisher Scientific (1 mentions) Cd31 nb100 2284, by Novus Biologicals (1 mentions) Hcytomag 60k, by Merck Group (1 mentions) Advia centaur xp, by Siemens (1 mentions) Bio plex manager software version 6, by Bio-Rad (1 mentions) Bio plex software, by Bio-Rad (1 mentions) Paxgene tube, by BD (1 mentions) Vacutainer serum tube, by BD (1 mentions) Bioplex promagnetic bead based assays, by Bio-Rad (1 mentions) Anti ccp2 elisa, by Axis-Shield (1 mentions)

Close spelling variants of this product

Bio-Plex 200 detection platform Bio-Plex MAGPIX platform Bio-Plex 200 System platform Bio-Plex 200 platform Luminex Bio-Plex 200 platform Bio-Plex

37 protocols using bio plex platform

1

Cytokine Profiling in TB-IRIS Patients

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The plasma concentrations of 18 cytokines and chemokines were measured in 33 TB-IRIS and 30 non-IRIS patients in week 0 and week 2 samples. TNF-α, IFN-γ, IFN-α2 and IL-1β, IL-6, IL-8 and IL-12p40 were quantified on the Bio-Plex platform (Bio-Rad Laboratories, Hercules, CA), using customized Milliplex kits (HCYTMAG-60 K, Merck Millipore, Germany). The concentration of IL-1α was measured by enzyme-linked immunorsorbent assay (eBioscience, San Diego, CA).
Lai R.P., Meintjes G., Wilkinson K.A., Graham C.M., Marais S., Van der Plas H., Deffur A., Schutz C., Bloom C., Munagala I., Anguiano E., Goliath R., Maartens G., Banchereau J., Chaussabel D., O'Garra A, & Wilkinson R.J. (2015). HIV–tuberculosis-associated immune reconstitution inflammatory syndrome is characterized by Toll-like receptor and inflammasome signalling. Nature Communications, 6, 8451.
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2

Multiplex Cytokine Profiling in Serum and Saliva

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The levels of 10 host markers were evaluated in serum and saliva samples for all study participants using a 10 plex customized kit from Bio-rad Laboratories (Hercules, CA, USA) on the Bio-Plex platform (Bio Rad). These included interferon gamma (IFN-γ), interleukin (IL) -2, 5, 6, granulocyte colony stimulating factor (G- CSF), granulocyte monocyte colony stimulating factor (GM-CSF), macrophage inflammatory protein (MIP)-1α and β, vascular endothelial growth factor (VEGF) and tumor necrosis factor alpha (TNF-α). As recommended by the manufacturer, the serum and saliva samples were diluted 1:4. Samples from one study participant were tested on the same plate. The Bio Plex Manager software, version 6.1 was used for bead acquisition and analysis of median fluorescent intensity.
Namuganga A.R., Chegou N.N., Mubiri P., Walzl G, & Mayanja-Kizza H. (2017). Suitability of saliva for Tuberculosis diagnosis: comparing with serum. BMC Infectious Diseases, 17, 600.
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3

Multiplex Immune Protein Profiling

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The preconfigured multiplex Human Immune Monitoring 65-plex ProcartaPlex immunoassay kit (Invitrogen, Thermo Fisher Scientific, UK) was used to measure 65 protein targets in plasma on the Bio-Plex platform (Bio-Rad Laboratories, Hercules, CA, USA), using Luminex xMAP technology. Analytes measured included APRIL; BAFF; BLC; CD30; CD40L; ENA-78; Eotaxin; Eotaxin-2; Eotaxin-3; FGF-2; Fractalkine; G-CSF; GM-CSF; Gro-Alpha; HGF; IFN-Alpha; IFN-gamma; IL-10; IL-12p70; IL-13; IL-15; IL-16; IL-17A; IL-18; IL-1Alpha; IL-1Beta; IL-2; IL-20; IL-21; IL-22; IL-23; IL-27; IL-2R; IL-3; IL-31; IL-4; IL-5; IL-6; IL-7; IL-8; IL-9; IP-10; I-TAC; LIF; MCP-1; MCP-2; MCP-3; M-CSF; MDC; MIF; MIG; MIP-1Alpha; MIP-1Beta; MIP-3Alpha; MMP-1; NGF-Beta; SCF; SDF-1Alpha; TNF-Beta; TNF-Alpha; TNF-R2; TRAIL; TSLP; TWEAK; VEGF-A. All assays were conducted as per the manufacturer’s recommendation.
Fendler A., Au L., Shepherd S.T., Byrne F., Cerrone M., Boos L.A., Rzeniewicz K., Gordon W., Shum B., Gerard C.L., Ward B., Xie W., Schmitt A.M., Joharatnam-Hogan N., Cornish G.H., Pule M., Mekkaoui L., Ng K.W., Carlyle E., Edmonds K., Del Rosario L., Sarker S., Lingard K., Mangwende M., Holt L., Ahmod H., Stone R., Gomes C., Flynn H.R., Agua-Doce A., Hobson P., Caidan S., Howell M., Wu M., Goldstone R., Crawford M., Cubitt L., Patel H., Gavrielides M., Nye E., Snijders A.P., MacRae J.I., Nicod J., Gronthoud F., Shea R.L., Messiou C., Cunningham D., Chau I., Starling N., Turner N., Welsh L., van As N., Jones R.L., Droney J., Banerjee S., Tatham K.C., Jhanji S., O’Brien M., Curtis O., Harrington K., Bhide S., Bazin J., Robinson A., Stephenson C., Slattery T., Khan Y., Tippu Z., Leslie I., Gennatas S., Okines A., Reid A., Young K., Furness A.J., Pickering L., Gandhi S., Gamblin S., Swanton C., Nicholson E., Kumar S., Yousaf N., Wilkinson K.A., Swerdlow A., Harvey R., Kassiotis G., Larkin J., Wilkinson R.J, & Turajlic S. (2021). Functional antibody and T-cell immunity following SARS-CoV-2 infection, including by variants of concern, in patients with cancer: the CAPTURE study. Research Square.
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4

NICHE-Mediated Localized Immunosuppression

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F344 rats (n = 4) were implanted with 2 NICHE (1 in each flank), loaded with 5 × 105 BM-MSCs and vascularized for 6 weeks. For each rat, one NICHE drug reservoir was loaded with 9 mg CTLA4Ig (30 mg/mL) and 0.6 mg ALS (2 mg/mL) for local Immunosuppressant (IS) release NICHE. The contralateral NICHE drug reservoir was loaded with saline and served as a control. Two weeks later, NICHE were harvested and processed for histology and VEGF quantification. For functional blood vessel staining, following pressure-cooker-based epitope retrieval, sections were stained with primary antibodies CD31 (NB100-2284, Novus Biologicals, 1:200), VE Cadherin (36-1900, Invitrogen, 1:25), and eNOS (ab300071, Abcam, 1:50). Anti-rabbit Alexa Flour 555 (A-21428, Invitrogen, 1:200) was used as secondary antibody. Imaging was performed using a Nikon Eclipse TE300 Fluorescent Microscope in the FL2 channel (585 ± 20 nm). Fluorescence intensity was measured by 2 independent observers using the NIS-Elements Basic Research software. VEGF was quantified from tissue lysate using Bio-Plex Pro Rat Cytokine VEGF Set (#171L1026M) magnetic bead-based assay (Bio-Rad Laboratories) on the Bio-plex platform (Bio-Rad), according to manufacturer’s instructions.
Paez-Mayorga J., Campa-Carranza J.N., Capuani S., Hernandez N., Liu H.C., Chua C.Y., Pons-Faudoa F.P., Malgir G., Alvarez B., Niles J.A., Argueta L.B., Shelton K.A., Kezar S., Nehete P.N., Berman D.M., Willman M.A., Li X.C., Ricordi C., Nichols J.E., Gaber A.O., Kenyon N.S, & Grattoni A. (2022). Implantable niche with local immunosuppression for islet allotransplantation achieves type 1 diabetes reversal in rats. Nature Communications, 13, 7951.
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5

Urinary Cytokine Quantification

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Urine levels of mature IL-1β were assayed on the BioPlex platform (BioRad, Luminex Technology, Hercules, CA). Creatinine was assayed using the Urinary Creatinine Kit from Cayman Chemical (Ann Arbor, MI). Cytokine levels were adjusted for creatinine and reported as pg cytokine/mg creatinine.
Hughes FM J.r., Kennis J.G., Youssef M.N., Lowe D.W., Shaner B.E, & Purves J.T. (2016). The NACHT, LRR and PYD Domains-Containing Protein 3 (NLRP3) Inflammasome Mediates Inflammation and Voiding Dysfunction in a Lipopolysaccharide-Induced Rat Model of Cystitis. Journal of clinical & cellular immunology, 7(1), 396.
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6

Cytokine Profiling in Rat Sera

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The levels of inflammatory cytokines including tumor necrosis factor (TNF‐α), interleukin (IL)‐1α, interleukin (IL)‐4, interleukin (IL)‐10, and interleukin (IL)‐18 were measured in sera from all rats. This was performed using a Bio‐Plex Pro™ magnetic bead‐based assays (Bio‐Rad, RRID: AB_2857368; https://scicrunch.org/resolver/RRID:AB_2857368) on the Bio‐Plex® platform (Bio‐Rad) according to the manufacturer's instruction. Following optimization, samples were evaluated undiluted in a blinded manner. Bio‐Plex Manager™ software, version 6.0 was used for bead acquisition and analysis. The lower limit of sensitivity was 3 pg/ml, 1 pg/ml, 1 pg/ml, 5 pg/ml, and 4 pg/ml for TNF‐α, IL‐1α, IL‐4, IL‐10, and IL‐18, respectively.
Li H., Zhang G., Guo Y., Deng J., Fischer H., Craig L.B., Kem D.C, & Yu X. (2020). Autoimmune activation of the GnRH receptor induces insulin resistance independent of obesity in a female rat model. Physiological Reports, 8(24), e14672.
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7

Multiplex Cytokine Profiling from Serum

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The evaluation of the various cytokine levels was performed on triplicate serum samples by using multiplex technology. The samples were removed from −80°C and allowed to thaw at room temperature prior to the assay. The analysis of cytokine levels was achieved by using the Bio-Plex Pro™ Reagent kit in 1 × 96-well flat-bottomed plate, Cat. #171-304070M (USA). The assays were performed in accordance with the product protocol manual (Merck Millipore, Billerica, MA, USA) and the Bio-Plex platform (Bio-Rad Laboratories, Hercules, CA, USA) was used to read the plate.
Arokoyo D.S., Oyeyipo I.P., Du Plessis S.S., Chegou N.N, & Aboua Y.G. (2018). Modulation of Inflammatory Cytokines and Islet Morphology as Therapeutic Mechanisms of Basella alba in Streptozotocin-Induced Diabetic Rats. Toxicological Research, 34(4), 325-332.
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8

Quantification of Cytokine and Chemokine Profile

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The concentration of chemokines and cytokines in CD14+ culture supernatants was quantified by immuno-fluorescence assay using the Multiplex Kit panel I and II (Millipore UK) on a Bio-Plex platform (Bio-Rad). The plates were designed to measure TNF-α, IL-1β, IL-10, CCL2/MCP-1, CCL3/MIP-1α, CCL4/MIP-1β, CCL5/RANTES, CCL7/MCP-3, CCL8/MCP-2, CCL13/MCP4, CCL17/TARC, CCL19/MIP-3β, CCL20/MIP-3α, CCL21/6CKINE, CCL22/MDC, CXCL1/GRO, CXCL5/ENA-78, CXCL7/NAP-2, CXCL8/IL-8, CXCL9/MIG, CXCL10/IP-10, CXCL11/I-TAC, CXCL12/SDF-1, CX3CL1/Fractalkine, lymphotactin and vascular endothelial growth factor A (VEGFA).
Elmesmari A., Fraser A.R., Wood C., Gilchrist D., Vaughan D., Stewart L., McSharry C., McInnes I.B, & Kurowska-Stolarska M. (2016). MicroRNA-155 regulates monocyte chemokine and chemokine receptor expression in Rheumatoid Arthritis. Rheumatology (Oxford, England), 55(11), 2056-2065.
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9

Multiplexed Analysis of Biomarkers

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Mediators analysed in undiluted plasma (P), pericardial fluid (PF) and saliva (S) samples included IFN-gamma, IL-1alpha, IL-1beta, IL-6, IL-10, IL-12p40, TNF, CXCL8 (IL-8) and CXCL10 (IP-10), using customized Milliplex™ kits (HCYTOMAG-60 K, Millipore, St Charles, MO, USA) on the Bio-Plex platform (Bio-Rad Laboratories, Hercules, CA, USA) as described [8] (link). Caspases 3, 8 and 9 in undiluted pericardial fluid and plasma were measured using human in vitro ELISA kits from Abcam (Cambridge, UK), following the manufacturer's recommendations.
Shenje J., Lai R.P., Ross I.L., Mayosi B.M., Wilkinson R.J., Ntsekhe M, & Wilkinson K.A. (2017). Effect of prednisolone on inflammatory markers in pericardial tuberculosis: A pilot study. International Journal of Cardiology. Heart & Vasculature, 18, 104-108.
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10

Multiplex Assay of QFT Plasma

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QFT plasma samples from unstimulated (Nil) and TB-specific, antigen-stimulated (Ag) tubes were stored at -80oC until batched analyses for the analytes listed in Supplementary Table 1. Undiluted (Ag and Nil) QFT plasma samples were assayed on 11 plates using customized MilliplexTM kits (Millipore, St Charles, MO) on the Bio-Plex platform (Bio-Rad Laboratories, Hercules, CA). The upper limit of detection for all analytes was 10 000 pg/ml, while the mean lower limit of detection from the 11 plates was as follows (all in pg/ml): epidermal growth factor (EGF), 8; IFN-α2, 6; interleukin (IL)-1α, 3; C-X-C motif chemokine 10 (CXCL10), 10; chemokine (C-C motif) ligand (CCL) 3, 7; CCL4, 3; soluble CD40 ligand (sCD40L), 16; transforming growth factor-α (TGF-α), 3; tumor necrosis factor (TNF)-α, 3; vascular endothelial growth factor (VEGF), 205; IFN-γ, 3; IL-2, 3; and IL-10, 3.
Lesosky M., Rangaka M.X., Pienaar C., Coussens A.K., Goliath R., Mathee S., Mwansa-Kambafwile J., Maartens G., Wilkinson R.J, & Wilkinson K.A. (2018). Plasma Biomarkers to Detect Prevalent or Predict Progressive Tuberculosis Associated With Human Immunodeficiency Virus–1. Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America, 69(2), 295-305.
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