Phosphodiesterase 1 crotalus adamanteus venom

O-(Pyridin-3-yl-methyl)hydroxylamine (PMOA), butyraldehyde, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), tris(hydroxymethyl)aminomethane (Tris), EDTA, DNase I (type IV, bovine pancreas), alkaline phosphatase (Escherichia coli), nuclease P1 (Penicillium citrinum), RNase A (bovine pancreas), RNase T1 (Aspergillus oryzae), and proteinase K (Tritirachium album) were purchased from Sigma-Aldrich (St. Louis, MO). Phosphodiesterase I (Crotalus adamanteus venom) and adenosine deaminase were purchased from Worthington Biochemical Corp. (Lakewood, NJ). LC-MS grade solvents were purchased from Fisher Chemical Co. (Pittsburgh, PA). Puregene protein precipitation (PP) solution and DNeasy Blood & Tissue kit were purchased from Qiagen (Germantown, MD). N,N-Bis(2-chloroethyl)ethylamine hydrochloride was purchased from Aaron Chemistry Gmbh (Mittenwald, Germany). Pall 10 kDa filter with omega membrane, MWCO 10 kDa, was purchased from Sigma Aldrich (St. Louis, MO) All other chemicals were ACS grade and purchased from Sigma-Aldrich unless otherwise stated. Strata-X 33 μm Polymeric Reversed Phase 30 mg solid-phase extraction (SPE) cartridge was purchased from Phenomenex (Torrance, CA). Sola SCX cartridges were purchased from Thermo Scientific (Bellafonte, PA). MicroLiter autosampler vials with silanized glass inserts were purchased from Wheaton (Millville, NJ).

Calf thymus (CT) DNA, RNase A (bovine pancreas), RNase T1 (Aspergillus oryzae), Proteinase K (Tritirachium album), DNase I (type IV, bovine pancreas), alkaline phosphatase (Escherichia coli), nuclease P1 (Penicillium citrinum) were purchased from Sigma-Aldrich (St. Louis, MO). Phosphodiesterase I (Crotalus adamanteus venom) was purchased from Worthington Biochemical Corp. (Newark, NJ). Maxwell 16 FFPE Plus LEV DNA purification kit was purchased from Promega Co. (Madison, WI). Unlabeled and stable isotopically labeled internal standards of [¹³C₁₀]-dG-C8-PhIP, [¹³C₁₀]-dG-C8-4-ABP, [¹³C₁₀]-dG-C8-AαC, [²H₃C]-dG-C8-MeIQx and [¹³C₁₀]-dG-N²-B[a]PDE were synthesized as described.^{37 (link),38 (link)}

4-ABP, calf thymus DNA (CT-DNA), Dimethyl sulfoxide (DMSO), Proteinase K (Tritirachium album), DNase I (type IV, bovine pancreas), alkaline phosphatase (Escherichia coli), nuclease P1 (Penicillium citrinum, RNase A (bovine pancreas), and RNase T₁ (Aspergillus oryzae) were purchased from Sigma-Aldrich (St. Louis, MO). Phosphodiesterase I (Crotalus adamanteus venom) was purchased from Worthington Biochemical Corp. (Newark, NJ). Isopore™ membrane filter (10 µm) and Sterifil^® Aseptic filter holder (500 mL) were purchased from Millipore-Sigma (Bedford, MA). 8-Methoxy-6-nitrophenanthro-[3,4-d]-1,3-dioxole-5-carboxylic (aristolochic acid, AA-I) was provided by Dr. H. Priestap, Department of Biological Sciences, Florida International University. The DNA adducts 7-(2′-deoxyadenosin-N⁶-yl)aristolactam-I (dA-AL-I) and [¹⁵N₅]-dA-AL-I,^{33 (link)}N-(2′-deoxyguanosin-8-yl)-4-aminobiphenyl (dG-C8–4-ABP), and [¹³C₁₀]-dG-C8–4-ABP were synthesized as described.^{34 (link)} DNeasy blood/tissue kit was purchased from Qiagen (Germantown, MD). Microliter CapLC vials with silanized inserts were purchased from Wheaton (Millville, NJ).