The cells lysates were added with sample buffer and boiled at 95°C for 5 min. Protein samples were separated using 10% sodium dodecyl sulphate‐polyacrylamide gel electrophoresis (SDS‐PAGE) and transferred onto nitrocellulose membranes. The membranes were blocked by 5% BSA for 30 min. and incubated with the primary antibodies at 4°C overnight, followed by incubation with secondary antibodies. Proteins were visualized using an enhanced chemiluminescence method kit (GE Healthcare Life Sciences, Piscataway, NJ, USA), and the protein band intensity was quantified via densitometric analysis using Quantity One software (Bio‐Rad Laboratories, Inc., Hercules, CA, USA).
Enhanced chemiluminescence method kit
Manufactured by GE Healthcare
Sourced in United States
The Enhanced chemiluminescence method kit is a laboratory equipment product designed to facilitate the detection and analysis of proteins using a chemiluminescence-based technique. This kit provides the necessary reagents and materials to perform this analytical method.
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Lab products found in correlation
2 protocols using enhanced chemiluminescence method kit
1
Western Blot Protocol for Protein Analysis
2
Immunoblotting of GPD1L and GAPDH
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Total protein was dissolved in radio-immunoprecipitation assay buffer (150 mm NaCl, 10 mm Tris, pH 7.2, 5 mm EDTA, 1% Triton X-100, 0.1% SDS, 1% deoxycholic acid). Protein samples were separated using 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred to nitrocellulose membranes. The membranes were blocked using 5% BSA for 30 min and incubated with diluted (1:1,000) polyclonal antibodies specific for GPD1L and GAPDH overnight at 4°C, followed by hybridized with secondary antibodies. After washing 3 times with TBST, the specific proteins were visualized using an enhanced chemiluminescence method kit (GE Healthcare Life Sciences, Piscataway, NJ).
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