The UV spectrum was recorded on a Jasco V-560 spectrophotometer (JASCO Corporation, Kyoto, Japan). Optical rotation was obtained on an Autopol IV Polarimeter (Rudolph Research Analytical, Flanders, NJ, USA). CD spectrum was acquired on a Jasco J-810-150S spectropolarimeter (JASCO Corporation, Japan). High-resolution electrospray ionization mass spectrometry (HRESIMS) data were collected on an AB Sciex Triple TOF 4600 mass spectrometer (AB SCIEX, Framingham, MA, USA). NMR spectra were recorded on a Bruker Avance II 500 MHz NMR spectrometer (Bruker, Karlsruhe, Germany) with tetramethylsilane (TMS) as an internal standard. Agilent 1260 Infinity (Agilent Technologies Inc., Santa Clara, CA, USA), Waters 2535 (Waters Corporation, Milford, MA, USA), and Shimadzu LC-20AR (Shimadzu Corporation, Kyoto, Japan) semi-preparative HPLC systems were created using a Welch Ultimate XB-C18 column (250 mm × 10.0 mm, 5 μm). Silica gel (100−200 mesh and 200−300 mesh, Qingdao Marine Chemical Ltd., Qingdao, China) and Sephadex LH-20 (GE Healthcare Bio-Sciences AB, Uppsala, Sweden) were used for column chromatography. The Silica gel GF254 (Qingdao Marine Chemical Co., Ltd., Qingdao, China) was used for analytical and preparative thin-layer chromatography (TLC).
Waters 2535
Manufactured by Waters Corporation
Sourced in United States
The Waters 2535 is a liquid chromatography system that provides reliable and precise separations. It features a quaternary gradient pump, an integrated sample manager, and a variety of detection options to meet the needs of various analytical applications.
Automatically generated - may contain errors
Lab products found in correlation
Silica gel, by Qingdao Marine Chemical (2 mentions)
Triple tof 4600 mass spectrometer, by AB Sciex (1 mentions)
Agilent 1260 infinity, by Agilent Technologies (1 mentions)
Sephadex lh 20, by Cytiva (1 mentions)
V 560 spectrophotometer, by Jasco (1 mentions)
Lc 20ar, by Shimadzu (1 mentions)
Autopol 4 polarimeter, by Rudolph Research Analytical (1 mentions)
Welch ultimate xb c18 column, by Hill-Rom (1 mentions)
Uv 2501pc spectrophotometer, by Shimadzu (1 mentions)
Avance 3 600 spectrometer, by Bruker (1 mentions)
810 spectropolarimeter, by Jasco (1 mentions)
Model 241 polarimeter, by PerkinElmer (1 mentions)
Pack ods a column, by YMC (1 mentions)
983g spectrometer, by PerkinElmer (1 mentions)
Silica gel tlcplates, by Qingdao Marine Chemical (1 mentions)
2 protocols using waters 2535
1
Analytical Techniques for Compound Characterization
2
Spectroscopic Analysis of Molecular Compounds
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The UV
spectra
were measured on a Shimadzu UV-2501 PC spectrophotometer. The specific
optical rotations were determined by a PerkinElmer polarimeter model
241, and IR spectra were performed by using a PerkinElmer 983 G spectrometer
(PerkinElmer, Inc., Waltham, MA, USA). The ECD spectra were recorded
on a JASCO 810 spectropolarimeter. The NMR spectra were obtained by
using a Bruker AVANCE III 600 spectrometer (Karlsruhe, Germany), and
HRESI mass spectra were determined by using Micromass Q-TOF2 spectrometer
(AB SCIEX, Canada). Semipreparative HPLC was performed by using Waters
2535 (Waters, Milford, MA, USA) with the YMC-Pack ODS-A column (5
μm,
10 × 250 mm, YMC, Kyoto, Japan), which contained a Waters 2489
UV detector. Its flow rate was 2.0 mL/min and the wavelength for detection
was 223 nm. The MPLC system was carried out by using a Büchi
Flash chromatography with a C-650 pump and a flash column (460 mm
× 26 mm i.d., Büchi Corp., Flawil, Switzerland), while
its flow rate was 15.0 mL/min. Sephadex LH-20 column chromatography
was purchased from GE Corp. D101 macroporous resin was acquired from
Xi’an Sunresin New Material Co. Ltd. Silica gel (200–300
mesh) and Silica gel TLC plates were obtained from Qingdao Marine
Chemical Factory.
spectra
were measured on a Shimadzu UV-2501 PC spectrophotometer. The specific
optical rotations were determined by a PerkinElmer polarimeter model
241, and IR spectra were performed by using a PerkinElmer 983 G spectrometer
(PerkinElmer, Inc., Waltham, MA, USA). The ECD spectra were recorded
on a JASCO 810 spectropolarimeter. The NMR spectra were obtained by
using a Bruker AVANCE III 600 spectrometer (Karlsruhe, Germany), and
HRESI mass spectra were determined by using Micromass Q-TOF2 spectrometer
(AB SCIEX, Canada). Semipreparative HPLC was performed by using Waters
2535 (Waters, Milford, MA, USA) with the YMC-Pack ODS-A column (5
μm,
10 × 250 mm, YMC, Kyoto, Japan), which contained a Waters 2489
UV detector. Its flow rate was 2.0 mL/min and the wavelength for detection
was 223 nm. The MPLC system was carried out by using a Büchi
Flash chromatography with a C-650 pump and a flash column (460 mm
× 26 mm i.d., Büchi Corp., Flawil, Switzerland), while
its flow rate was 15.0 mL/min. Sephadex LH-20 column chromatography
was purchased from GE Corp. D101 macroporous resin was acquired from
Xi’an Sunresin New Material Co. Ltd. Silica gel (200–300
mesh) and Silica gel TLC plates were obtained from Qingdao Marine
Chemical Factory.
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