Integrin α5 (clone 5H10-27(MFR5)) and integrin αV (clone RMV-7) antibodies were purchased from BD Biosciences. Integrin β1 (clone HMβ1-1) was obtained from Biolegend and integrin β3 antibody (clone HMb3-1) from Millipore (MA, US). The secondary antibodies [biotin-SP-conjugated goat α-rat IgG, Rhodamine Red-X-AffiniPure goat α-Armenian hamster IgG(H + L)] and streptavidin-FITC were purchased from Jackson ImmunoResearch (West Grove, PA). For quantification of surface integrin expression, detached fibroblasts were incubated in suspension medium (DMEM containing 0.25% BSA) for 40 min at 37°C. Then, 2 × 105 cells were incubated with appropriate primary antibodies (diluted 1:300) in FACS buffer (5% heat-inactivated FCS, 1% sodium azide in PBS) for 1 hr at 4°C. After washing, secondary antibodies were applied for 1 h at 4°C, then washed again, before samples were analyzed by flow cytometry (LSRII, BD Biosciences).
Integrin β1 clone hmβ1 1
Manufactured by BioLegend
Sourced in United States
Integrin β1 (clone HMβ1-1) is a monoclonal antibody that binds to the integrin beta 1 subunit. Integrins are cell surface receptors involved in cell-cell and cell-extracellular matrix interactions.
Automatically generated - may contain errors
2 protocols using integrin β1 clone hmβ1 1
1
Quantifying Surface Integrin Expression
2
Quantifying Cell Surface Integrin Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Integrin α 5 (clone 5H10-27(MFR5)) and integrin α V (clone RMV-7) antibodies were purchased from BD Biosciences. Integrin β 1 (clone HMβ1-1) was obtained from Biolegend and integrin β 3 antibody (clone HMb3-1) from Millipore (MA, US). Secondary antibodies (biotin-SP-conjugated goat α-rat IgG), streptavidin-FITC, and Rhodamine Red-X-AffiniPure Goat Anti-Armenian Hamster IgG (H+L) were purchased from Jackson ImmunoResearch (West Grove, PA). For quantification of surface integrin expression, suspended fibroblasts were incubated in suspension medium (DMEM containing 0.25% BSA) for 40 min at 37℃. Then, 2 × 10 5 cells were incubated with appropriate primary antibodies (diluted 1:300) in FACS buffer (5% heat-inactivated FCS, 1% sodium azide in PBS) for 1 hr at 4℃.
After washing, secondary antibodies were applied for 1 hr at 4℃, then washed again, samples were analyzed by flow cytometry (LSRII, BD Biosciences).
After washing, secondary antibodies were applied for 1 hr at 4℃, then washed again, samples were analyzed by flow cytometry (LSRII, BD Biosciences).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!