The largest database of trusted experimental protocols

3 protocols using snu 175

1

Cell Culture Protocols for Colon Cancer

Check if the same lab product or an alternative is used in the 5 most similar protocols
HCT-15, DLD-1, HCT-8, SW480, SNU-175, SNU-C5, HT-29, Caco-2, and SNU-C4 cells were purchased from the Korean Cell Line Bank (Seoul, Korea). HCT-15, DLD-1, HCT-8, SW480, SNU-175, SNU-C5, HT-29, and SNU-C4 cells were maintained in RPMI 1640 with 10% FBS, 4mM L-glutamine, and 1% penicillin/streptomycin at 37 °C with 5% CO2. Caco-2 cells were maintained in DMEM with 10% FBS, 4mM L-glutamine, and 1% penicillin/streptomycin at 37 °C with 5% CO2. Normal colon epithelial CCD8410CoN cell line was kindly provided by Prof. Nayoung Kim at Seoul National University, Seoul, Korea. CCD841CoN cells were maintained in an Eagle’s Minimum Essential Medium (EMEM) with 10% FBS, 4mM L-glutamine, and 1% penicillin/streptomycin at 37 °C with 5% CO2.
+ Open protocol
+ Expand
2

Establishment and Characterization of Human CRC Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
The human CRC cell lines Caco-2, COLO-320DM, DLD-1, HCT-8, HCT-15, HT-29, LoVo,
SW480, SNU-C2B, SNU-C5, and SNU-175 were purchased from the Korean Cell Line Bank
(Seoul, Korea). The DiFi cell line was kindly provided by Dr. J. O. Park (Samsung
Medical Center, Seoul, Korea). The KRAS mutation status of all cell
lines are summarized in Table 1[12 -15 (link)]. All cell lines were
cultured in RPMI-1640 medium (Gibco, Grand Island, NY), supplemented with 10% fetal
bovine serum (FBS; Gibco) and 1% penicillin/streptomycin solution (WelGENE Inc.,
Daegu, Korea), and were maintained at 37°C in 5% atmospheric
CO2.
+ Open protocol
+ Expand
3

Culturing Human CRC Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human CRC cell lines (colo-201, colo-205, colo-320, HCT-15, HCT-116, HT-29, Lovo, LS174T, SNU-61, SNU-70, SNU-81, SNU-175, SNU-254, SNU-283, SNU-407, SNU-977, SNU-1033, SNU-1181, SNU-1235, SNU-1411, SNU-1544, SNU-1684, SNU-C1, SNU-C2A, SNU-C4, SNU-C5, SW-403, and SW-480) were purchased from the Korean Cell Line Bank [18 (link)]. All cell lines were cultured in RPMI1640 (Hyclone Laboratories, Ind., Logan, UT), supplemented with 10% fetal bovine serum, at 37°C in humidified 5% CO2.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!