2 2 dyphenyl 1 picrylhydrazyl dpph

Manufactured by Merck Group

Sourced in United States

2,2-dyphenyl-1-picrylhydrazyl (DPPH) is a stable free radical compound commonly used in laboratory settings as a reagent. Its core function is to measure the antioxidant capacity of various substances through a colorimetric assay. DPPH exhibits a deep purple color that changes to yellow upon reduction, allowing for the quantification of antioxidant activity.

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3 protocols using 2 2 dyphenyl 1 picrylhydrazyl dpph

Antioxidant Characterization of Organic Solvents

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Organic solvents and sodium hydrogen carbonate were purchased from Zorka pharma Sabac, Serbia. 2,2-dyphenyl-1-picrylhydrazyl (DPPH) was obtained from Sigma Chemicals Co., St. Louis, MO, USA. Folin-Ciocalteu phenol reagent, 3-tert-butyl-4-hydroxyanisole (BHA), and aluminium chloride hexahydrate (AlCl₃·6H₂O) were purchased from Fluka Chemie AG, Buchs, Switzerland. All other solvents and chemicals were of analytical grade.

Zlatić N.M, & Stanković M.S. (2017). Variability of Secondary Metabolites of the Species Cichorium intybus L. from Different Habitats. Plants, 6(3), 38.

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Comparative Analysis of Green Tea Varieties

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Three kinds of pure green tea (tea bags), the first two were popular Korean based brands and the third one, a well known international brand. First green tea was iced green tea (emart.kr), the second is Boseong green tea and the third one is Lipton green tea. These were purchased from a local supermarket in Seoul, Korea. Millipore water was used for all experiments. Standards of total phenolic acids (gallic acid) were procured from MP Biomedicals, LLC, Illkirch, France and of flavanoids (rutin hydrate) from abcam Biochemicals, Cambridge Science Park Cambridge CB4 0FL UK. Trolox was purchased from Enzo Life sciences Inc., Farmingdale, NY. 2,2-dyphenyl-1-picrylhydrazyl (DPPH) was obtained from Sigma Aldrich Company, St Louis, MO 63103, USA. The Folin- Ciocalteu’s phenol reagent and aluminium chloride (AlCl3) were from MP Biomedicals, LLC, BP 50067 Illkirch, France. All other solvents and chemicals were of analytical grade.

Gopal J., Muthu M., Paul D., Kim D.H, & Chun S. (2016). Bactericidal activity of green tea extracts: the importance of catechin containing nano particles. Scientific Reports, 6, 19710.

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Extraction and Characterization of Phenolic Compounds

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Carbon dioxide (CO 2 , SFE grade), contained in a dip tube cylinder, was purchased from Air Products Sp, Poland. Methanol for HPLC-super gradient were purchased from POCh (Gliwice, Poland), Folin-Ciocalteu's reagent, and hydrochloric acid 35-38% purity was purchased from Chempur (Piekary Śl ąskie, Poland). Glacial acetic acid, 3,4,5-trihydroxybenzoic acid (gallic acid), and 2,2-dyphenyl-1-picrylhydrazyl (DPPH), iron (III) chloride, sodium acetate, sodium carbonate anhydrous, potassium acetate, phosphate buffered saline tablet, potassium persulphate, 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ), and 2,2 -azino-bis(3-ethylbenzothiazoline)-6-sulphonic acid (ABTS) were purchased from Sigma-Aldrich (St. Louis, MO, USA). All phenolic standards including trans-ferulic acid, trans-cinnamic acid, trans-hydroxycinnamic acid, syringic acid, synapic acid, vanillic acid, p-coumaric acid, caffeic acid, caffeic acid phenethyl ester (CAPE), kaempherol, quercetin, (+)-catechin, (-)-epicatechin, apigenin, rutin, myricetin, pinocenbrin, pinobanksin, pinostrobin, galangin, chrysin, and naringenin were purchased from Sigma-Aldrich (St. Louis, MO, USA). All chemicals were of analytical grade.

Uwineza P.A., Gramza‐Michałowska A., Bryła M., & Waśkiewicz A. (2021). Antioxidant Activity and Bioactive Compounds of Lamium album Flower Extracts Obtained by Supercritical Fluid Extraction. Applied Sciences, 11(16), 7419-7419.

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