Protein samples from co-immunoprecipitation were loaded onto SDS-polyacrylamide gels using SDS-sample buffer, separated by gel-electrophoresis and analysed by immunoblotting. All antibodies were diluted in 50 mM Tris [pH 7.2], 150 mM NaCl with 0.2% Tween-20 and 5% skim milk powder. Antibodies and dilutions are listed in Supplementary Table S1 . For visualization, we used Amersham ECL Prime or Select Western Blotting Detection Reagent (GE Healthcare) in combination with the Fusion FX-6 Edge system (Vilber Lourmat). Quantification was performed in a semi-automated manner using the ImageQuant TL 1D software (GE Healthcare Life Sciences) with a rolling-ball background correction.
Amersham ecl prime or select western blotting detection reagent
Manufactured by GE Healthcare
The Amersham ECL Prime or Select Western Blotting Detection Reagent is a chemiluminescent substrate used for the detection of proteins in Western blot analysis. It provides a sensitive and quantitative method for visualizing and analyzing target proteins.
Automatically generated - may contain errors
Lab products found in correlation
Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions)
Imagequant tl 1d software, by GE Healthcare (1 mentions)
Fusion fx 6 edge system, by Vilber (1 mentions)
Anti lc3, by Cell Signaling Technology (1 mentions)
Anti β tubulin, by Santa Cruz Biotechnology (1 mentions)
Anti tufm, by Thermo Fisher Scientific (1 mentions)
Anti sdha, by Cell Signaling Technology (1 mentions)
Anti cox 4, by Cell Signaling Technology (1 mentions)
Chemidoc imaging system, by Bio-Rad (1 mentions)
2 protocols using amersham ecl prime or select western blotting detection reagent
1
Immunoblotting Analysis of Protein Complexes
2
Mitochondrial Protein Profiling by Western Blot
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total cell lysates were prepared from cells. Briefly, cells were washed with PBS and resuspended in lysis buffer (50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 0.25% NP-40, 0.1% Triton X-100, 0.1% SDS and supplemented with protease inhibitors). Protein concentrations were measured with Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, cat. 23227). Equal amounts of protein were separated on SDS-PAGE and transferred to nitrocellulose or PVDF (for anti-LC3 antibody only) membrane. Membranes were probed with anti-MTCO1 (COX1, Abcam, cat. ab14705), anti-COX4 (Cell Signaling, cat. 4850), anti-SDHA (Cell Signaling, cat. 5839), anti-β-tubulin (Santa Cruz, cat. sc-53140), anti-TUFM (Thermo Fisher Scientific, cat. MA5-31363), anti-MRPS18A (Thermo Fisher Scientific, cat. PA5-57274), anti-LC3 (Cell Signaling, cat. 3868S), and secondary HRP-conjugated antibodies (Santa Cruz Biotechnology). Detection was performed using Amersham ECL Prime or Select Western Blotting Detection Reagent (GE Healthcare Life Sciences) and ChemiDoc Imaging System (Bio-Rad). Data were analyzed using ImageLab software.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!