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Anti cd134 bv711 clone act35

Manufactured by BD
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Anti-CD134–BV711 (clone ACT35) is a fluorescently-labeled monoclonal antibody that binds to the CD134 cell surface receptor. This product is intended for use in flow cytometry applications.

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Lab products found in correlation

Cytofix cytoperm fixation permeabilization solution kit, by BD (3 mentions) Rosiglitazone, by Merck Group (3 mentions) Fenofibrate, by Merck Group (3 mentions) Anti cd3 clone okt3, by Thermo Fisher Scientific (3 mentions) Anti cd69 fitc clone l78, by BD (3 mentions) Anti active caspase 3 pe clone c92 605, by BD (3 mentions)

3 protocols using anti cd134 bv711 clone act35

1

Anti-CD3 Stimulation and PPAR Modulation

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PBMCs were stimulated for 24 hr with plate-bound anti-CD3 (clone OKT3; Thermo Fisher Scientific) in the absence or presence of fenofibrate (50 μM; Sigma-Aldrich) or rosiglitazone (40 μM; Sigma-Aldrich). Activation markers were quantified on the cell surface using anti-CD69–FITC (clone L78; BD Biosciences) and anti-CD134–BV711 (clone ACT35; BD Biosciences). Intracellular stains were performed using anti-active caspase-3–PE (clone C92-605; BD Biosciences) in conjunction with a Cytofix/Cytoperm Fixation/Permeabilization Solution Kit (BD Biosciences).
Nicoli F., Cabral-Piccin M.P., Papagno L., Gallerani E., Fusaro M., Folcher V., Dubois M., Clave E., Vallet H., Frere J.J., Gostick E., Llewellyn-Lacey S., Price D.A., Toubert A., Dupré L., Boddaert J., Caputo A., Gavioli R, & Appay V. (2022). Altered basal lipid metabolism underlies the functional impairment of naive CD8+ T cells in elderly humans. Journal of immunology (Baltimore, Md. : 1950), 208(3), 562-570.
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2

Anti-CD3 Stimulation and PPAR Modulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
PBMCs were stimulated for 24 hr with plate-bound anti-CD3 (clone OKT3; Thermo Fisher Scientific) in the absence or presence of fenofibrate (50 μM; Sigma-Aldrich) or rosiglitazone (40 μM; Sigma-Aldrich). Activation markers were quantified on the cell surface using anti-CD69–FITC (clone L78; BD Biosciences) and anti-CD134–BV711 (clone ACT35; BD Biosciences). Intracellular stains were performed using anti-active caspase-3–PE (clone C92-605; BD Biosciences) in conjunction with a Cytofix/Cytoperm Fixation/Permeabilization Solution Kit (BD Biosciences).
Nicoli F., Cabral-Piccin M.P., Papagno L., Gallerani E., Fusaro M., Folcher V., Dubois M., Clave E., Vallet H., Frere J.J., Gostick E., Llewellyn-Lacey S., Price D.A., Toubert A., Dupré L., Boddaert J., Caputo A., Gavioli R, & Appay V. (2022). Altered basal lipid metabolism underlies the functional impairment of naive CD8+ T cells in elderly humans. Journal of immunology (Baltimore, Md. : 1950), 208(3), 562-570.
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3

Activation Markers and Apoptosis in PBMCs

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PBMCs were stimulated for 24 hr with plate-bound anti-CD3 (clone OKT3; Thermo Fisher Scientific) in the absence or presence of fenofibrate (50 μM; Sigma-Aldrich) or rosiglitazone (40 Sigma-Aldrich). The expression of the standard activation markers CD69 and CD134 (OX40) was quantified on the cell surface using anti-CD69-FITC (clone L78; BD Biosciences) and anti-CD134-BV711 (clone ACT35; BD Biosciences). Intracellular staining for activated caspase-3, used as marker of susceptibility to apoptosis upon activation, was performed using anti-active caspase-3-PE (clone C92-605; BD Biosciences) in conjunction with a Cytofix/Cytoperm Fixation/Permeabilization Solution Kit (BD Biosciences).
Nicoli F., Cabral-Piccin M.P., Papagno L., Gallerani E., Folcher V., Dubois M., Barrett A.J., Vallet H., Frere J.J., Gostick E., Llewellyn‐Lacey S., Price D.A., Toubert A., Boddaert J., Caputo A., Gavioli R., & Appay V. (2020). Altered basal lipid metabolism underlies the functional impairment of naive CD8+ T cells in elderly humans.
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