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Mscgm cd mesencymal stem cell growth medium bulletkit

Manufactured by Lonza
Sourced in Switzerland

MSCGM-CD™ Mesencymal Stem Cell Growth Medium BulletKit™ is a complete, serum-free, low-protein medium optimized for the growth and expansion of human mesenchymal stem cells (hMSCs) in culture.

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2 protocols using mscgm cd mesencymal stem cell growth medium bulletkit

1

Culturing hMSC-ATs using MSCGM-CD™ Medium

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The MSCGM-CD™ Mesencymal Stem Cell Growth Medium BulletKit™ was obtained from Lonza
(Basel, Switzerland). hMSC-ATs (46-year-old Caucasian female) (PromoCell, Heidelberg,
Germany) were cultured. Fetal bovine serum (FBS) was obtained from BioWest (Nuaille,
France). D-MEM/Ham’s F-12 medium was obtained from Wako (Osaka, Japan). Plastic dishes
were obtained from TPP (Trasadingen, Switzerland). All other materials used were of the
highest commercial grade.
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2

Mesenchymal Stem Cell Culture Optimization

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The MSCGM-CD™ Mesencymal Stem Cell Growth Medium BulletKit™ was obtained from Lonza (Basel, Switzerland). hADSCs (46-year-old/female/Caucasian) were obtained from PromoCell (Heidelberg, Germany). Fetal bovine serum (FBS) was obtained from BioWest (Nuaille, France). d-MEM (high glucose) with l-Glutamine, Phenol Red and Sodium Pyruvate (DMEM) was obtained from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Recombinant Human MFG-E8 Protein was obtained from R&D Systems, Inc. (Minneapolis, MN, USA). Lipopolysaccharide (LPS) from Escherichia coli O26:B6 was obtained from MilliporeSigma (St. Louis, MO, USA). Plastic dishes were obtained from TPP (Trasadingen, Switzerland). All other materials were of the highest commercial grade.
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