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Sodium heparin cpt tubes

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Sodium heparin CPT tubes are laboratory collection tubes used for the collection and transportation of blood samples. The primary function of these tubes is to prevent blood clotting by the addition of sodium heparin, an anticoagulant.

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Lab products found in correlation

Act diff hematology analyzer, by Beckman Coulter (2 mentions) Gibco ack buffer, by Thermo Fisher Scientific (2 mentions) Gemcell heat inactivated fetal bovine serum, by Gemini Bio (2 mentions)

Spelling variants of this product

Sodium heparin (88 citations) CPT tubes (67 citations) Heparin (64 citations) Sodium heparin tubes (44 citations) Heparin tubes (39 citations) BD Vacutainer CPT Mononuclear Cell Preparation Tubes with Sodium Heparin (6 citations) BD Vacutainer CPT Cell Preparation Tube with Sodium Heparin (2 citations) BD Vacutainer CPT Mononuclear Cell Preparation Tube-Sodium Heparin (2 citations)

2 protocols using sodium heparin cpt tubes

1

PBMC Isolation from SSc Patients

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SSc patients (n = 48) and healthy controls (n = 25) underwent venipuncture during which 32 mL of blood was collected in sodium heparin CPT tubes (BD Biosciences, San Jose CA), and PBMCs were isolated according to the manufacturer’s directions. Red blood cell lysis was performed using Gibco ACK Buffer (ThermoFisher). Purified PBMCs were counted using Ac-T Diff Hematology Analyzer (Beckman Coulter, Indianapolis, IN) and cryopreserved at 4–5 × 106 PBMCs/mL in GemCell heat inactivated fetal bovine serum (Gemini Bioproducts) using controlled rate cell freezing containers.
Wilfong E.M., Vowell K.N., Bunn K.E., Rizzi E., Annapureddy N., Dudenhofer R.B., Barnado A., Bonami R.H., Johnson J.E., Crofford L.J, & Kendall P.L. (2021). CD19 + CD21lo/neg cells are increased in systemic sclerosis-associated interstitial lung disease. Clinical and Experimental Medicine, 22(2), 209-220.
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2

Isolation and Cryopreservation of PBMCs

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SSc patients (n = 48) and healthy controls (n = 25) underwent venipuncture during which 32 mL of blood was collected in sodium heparin CPT tubes (BD Biosciences, San Jose CA), and PBMCs were isolated according to the manufacturer’s directions. Red blood cell lysis was performed using Gibco ACK Buffer (ThermoFisher). Purified PBMCs were counted using Ac-T Diff Hematology Analyzer (Beckman Coulter, Indianapolis, IN) and cryopreserved at 4–5 × 106 PBMCs/mL in GemCell heat inactivated fetal bovine serum (Gemini Bioproducts) using controlled rate cell freezing containers.
Wilfong E.M., Vowell K.N., Bunn K.E., Rizzi E., Annapureddy N., Dudenhofer R.B., Barnado A., Bonami R.H., Johnson J.E., Crofford L.J, & Kendall P.L. (2021). CD19 + CD21lo/neg cells are increased in systemic sclerosis-associated interstitial lung disease. Clinical and Experimental Medicine, 22(2), 209-220.
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