Log In Sign up
The largest database of trusted experimental protocols

Acetyl histone h3 lys23

Manufactured by Merck Group
Visit Supplier

Acetyl histone H3 Lys23 is a lab reagent used in the study of epigenetic modifications. It is a modified version of the histone H3 protein, with an acetyl group attached to the 23rd lysine residue. This modification is important for regulating gene expression and chromatin structure.

Automatically generated - may contain errors

Lab products found in correlation

Pias3, by Santa Cruz Biotechnology (1 mentions) Chip kit, by Cell Signaling Technology (1 mentions) Tri methyl histone h3 lys27, by Cell Signaling Technology (1 mentions) Tri methyl histone h3 lys4, by Cell Signaling Technology (1 mentions) Smad2, by Cell Signaling Technology (1 mentions) Smad3, by Cell Signaling Technology (1 mentions) Stat3, by Santa Cruz Biotechnology (1 mentions) Smad4, by Santa Cruz Biotechnology (1 mentions) Ab18521, by Abcam (1 mentions) Ab17036, by Abcam (1 mentions) Epiquik histone h3 modification multiplex assay kit, by Epigentek (1 mentions) Abe223, by Merck Group (1 mentions) Image software, by Bio-Rad (1 mentions) Acetyl histone h3 lys14, by Merck Group (1 mentions) Chemidoc mp imager, by Bio-Rad (1 mentions)

2 protocols using acetyl histone h3 lys23

1

Chromatin Immunoprecipitation Assay for Smad Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Chromatin was prepared from 1 × 107 CD4+ T cells isolated from C57BL/6 mice, Cd4Cre;Smad2fl/fl mice, Smad3−/− mice and the littermate control mice under TH17-polarizing condition for 3–4 days. Immunoprecipitation was performed with antibodies (1:50 dilution) against Smad2 (Cell Signaling Technology, Cat. no. 5339), Smad3 (Cell Signaling Technology, Cat. no. 9523), phospho-Smad3C S423/425 (Cell Signaling Technology, Cat. no. 9520), Smad4 (Santa Cruz, Cat. no. sc-7966), STAT3 (Santa Cruz, Cat. no. sc-7179), tri-methyl histone H3 Lys4 (Cell Signaling Technology, Cat. no. 9751), tri-methyl histone H3 Lys27 (Cell Signaling Technology, Cat. no. 9733), acetyl histone H3 Lys23 (Millipore, Cat. no. 17–10112) and PIAS3 (Santa Cruz) using ChIP kit (Cell Signaling Technology) according to the manufacturer's protocol. Immunoprecipitated DNA released from the crosslinked proteins was quantitated with real-time PCR using the primers (Supplementary Table 3) and was normalized to input DNA.
Yoon J.H., Sudo K., Kuroda M., Kato M., Lee I.K., Han J.S., Nakae S., Imamura T., Kim J., Ju J.H., Kim D.K., Matsuzaki K., Weinstein M., Matsumoto I., Sumida T, & Mamura M. (2015). Phosphorylation status determines the opposing functions of Smad2/Smad3 as STAT3 cofactors in TH17 differentiation. Nature Communications, 6, 7600.
+ Open protocol
+ Expand
2

Quantifying Histone Modifications via Immunoblot and ELISA

Check if the same lab product or an alternative is used in the 5 most similar protocols
Extracted histones were subjected to immunoblot analysis for H3/H4 acetylation and methylation marks using antibodies to acetyl-Histone H3 Lys4 (ABE223; Millipore), acetyl-Histone H3 Lys14 (04-1044; Millipore), acetyl-Histone H3 Lys18 (AB1191; Abcam), acetyl-Histone H3 Lys23 (07-360; Millipore), acetyl-Histone H4 Lys5 (07-327; Millipore), acetyl-Histone H4 Lys8 (61103; Active Motif), acetyl-Histone H4 Lys12 (07-595; Millipore), acetyl-Histone H4 Lys16 (07-329; Millipore), monomethyl-Histone H3 Lys4 (07-436; Millipore), monomethyl-Histone H3 Lys9 (ABE101-S; Millipore), unmethylated-Histone H3 Lys9 (MABE263; Millipore), monomethyl-Histone H3 Lys27 (07-448; Millipore), total Histone H3 (AB18521; Abcam) and total Histone H4 (AB17036; Abcam). Each measured protein was normalized to total H3 or H4. The chemiluminescent blots were imaged with a ChemiDoc MP imager (Bio-Rad) and quantified using Image software (NIH, Bethesda, MD). At the same time, an EpiQuik Histone H3 Modification Multiplex Assay Kit (P-3100; EpiGentek) was used to detect and quantify various H3 methylations simultaneously in an ELISA-like format following the manufacturer’s recommended protocols.
Ge Y., Zhu J., Wang X., Zheng N., Tu C., Qu J, & Ren X. (2018). Mapping dynamic histone modification patterns during arsenic-induced malignant transformation of human bladder cells. Toxicology and applied pharmacology, 355, 164-173.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!