Cd47 apc

Manufactured by Thermo Fisher Scientific

CD47-APC is a fluorescently labeled antibody used for the detection and analysis of CD47 expression on cells. CD47 is a surface protein involved in various cellular processes. This product is intended for research use only.

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APC-conjugated mouse anti-human CD47 MAb (clone B6H12, (2 citations)

2 protocols using cd47 apc

Quantifying SIRT1 Expression in CD34+ Cells

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CD34⁺ cells were labeled with CD34-PeCy7, Lin-APC-Cy7 (including CD2, CD3, CD7, CD10, CD19, and CD235a), and CD38-APC or CD38-PE, CD90-PercpCy5.5, CD123-APC, or CD47-APC (eBioscience) antibodies; fixed and permeabilized (Cytofix/Cytoperm Kit, Beckman Coulter); labeled with rabbit anti-human SIRT1 (E1104-1, Millipore) and Alexa Fluor 488-conjugated goat anti-rabbit antibodies (Invitrogen); and analyzed by flow cytometry. Data were analyzed using FlowJo software (version 8.5.2; TreeStar).

Li L., Osdal T., Ho Y., Chun S., McDonald T., Agarwal P., Lin A., Chu S., Qi J., Li L., Hsieh Y.T., Santos C.D., Yuan H., Ha T.Q., Popa M., Hovland R., Bruserud Ø., Gjertsen B.T., Kuo Y.H., Chen W., Lain S., McCormack E, & Bhatia R. (2014). SIRT1 Activation by a c-MYC Oncogenic Network Promotes the Maintenance and Drug Resistance of Human FLT3-ITD Acute Myeloid Leukemia Stem Cells. Cell stem cell, 15(4), 431-446.

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Multiparameter Flow Cytometry of Immune Cells

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Single cell suspensions were stained with monoclonal antibodies for 1 hour at 4°C. One to four‐color flow cytometry was performed (FACS LSR, Becton Dickinson). Data were acquired using the CellQuest software (Becton Dickinson) using at least 100 000 events gated for live cells.
The following conjugated rat anti‐mouse mAbs were used: CD45‐PerCP, CD11b‐APC, CCR4‐PE, CCR5‐PE, CCR7‐PE, CCR10‐PE and CXCR4‐PE (BD Biosciences); CD49b‐APC (Biolegend); CD226‐PE, CD4‐FITC, CD25‐APC, CD8a‐FITC, PD1‐PE, CTLA4‐PE, PDL1‐PE‐Cy7, PDL2‐FITC, CD47‐APC (eBiosciences), CD11c‐FITC, GR1‐FITC (Miltenyi Biotech), CD206 (Santa Cruz), and anti‐mouse Foxp3 staining set PE (eBioscience).

El Hafny‐Rahbi B., Brodaczewska K., Collet G., Majewska A., Klimkiewicz K., Delalande A., Grillon C, & Kieda C. (2021). Tumour angiogenesis normalized by myo‐inositol trispyrophosphate alleviates hypoxia in the microenvironment and promotes antitumor immune response. Journal of Cellular and Molecular Medicine, 25(7), 3284-3299.

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