Isolated retains were dissociated to a single cell level as described previously.22 (link) Peripheral blood was collected from tail veins, and the erythrocytes were lysed using ACK lysing buffer (Thermo Fisher Scientific). Cells were stained with PE-conjugated anti-CD11b antibody (Bio Legend, San Diego, CA, USA), Alexa Fluor647-conjugated anti-CD73 antibody (BD Biosciences), or Annexin V-PE (Bio Vison, Milpitas, CA, USA). Dead cell exclusion was done by using propidium iodide (Sigma-Aldrich). Cells were analyzed by FACS Calibur (BD Biosciences). Data analysis were performed using Flow Jo software (BD Biosciences).
Pe conjugated anti cd11b antibody
Manufactured by BioLegend
Sourced in United States
The PE-conjugated anti-CD11b antibody is a fluorescent-labeled monoclonal antibody that binds to the CD11b cell surface antigen. CD11b is expressed on the surface of various immune cells, including monocytes, macrophages, and neutrophils. This antibody can be used in flow cytometry applications to identify and characterize these cell types.
Automatically generated - may contain errors
2 protocols using pe conjugated anti cd11b antibody
1
Isolation and Analysis of Myeloid Cells
2
Isolated Retains Cell Profiling
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Isolated retains were dissociated to single cell level as described previously [20] . Peripheral blood were collected from tail vein, and the erythrocytes were lysed using ACK lysing buffer (Thermo Fisher Scienti c). Cells were stained with PE-conjugated anti-CD11b antibody (Bio Legend), Alexa Fluor647conjugated anti-CD73 antibody (BD Biosciences), or Annexin V-PE (Bio Vison). Dead cell exclusion was done by using propidium iodide (Sigma-Aldrich). Cells were analyzed by FACS Calibur (BD Biosciences). Data analysis were performed using Flow Jo software (BD Biosciences).
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