Cd efficient feed b

Manufactured by Thermo Fisher Scientific

Sourced in United States

CD Efficient Feed B is a laboratory equipment product designed to provide a consistent and efficient feed for cell culture applications. It is a specialized medium that supports the growth and proliferation of cells in controlled environments. The core function of this product is to supply the necessary nutrients and growth factors to sustain and optimize cell cultures.

Automatically generated - may contain errors

Lab products found in correlation

Freestyle max reagent, by Thermo Fisher Scientific (1 mentions) Cd forticho, by Thermo Fisher Scientific (1 mentions)

2 protocols using cd efficient feed b

Expressing UTI-Fc Fusions in CHO Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 3

Expression of UTI-Fc Fusions in CHO Cells.

A DNA vector encoding UTI-Fc1 was stably transfected into CHO-S cells using Invitrogen's Freestyle MAX Reagent. Bulk cultures were plated into T-flasks and selected using CD CHO supplemented with various concentrations of methionine sulfoximine (MSX) ranging from 50-100 μM. Once the cultures recovered from selection, they were expanded for production and cryopreservation. Multiple production batches were done to support in vitro and in vivo testing. The production process is a 10-14 day fed-batch culture using CD FortiCHO, CD Efficient Feed B, and CD Efficient Feed C from Invitrogen. The production volumes ranged from 1 L-3 L, and the cultures were harvested by centrifugation at 3500 rpm for 1-2 hours followed by sterile filtration of the supernatant and the resulting cell supernatant was used in purification.

US10351618B2. UTI fusion proteins (2019-07-16). Takeda Pharmaceutical Company Limited [JP]. Inventors: Aaron Chamberlain [US], Qiang Liu [US], Mathias Schmidt [US].

+ Open protocol

+ Expand

Recombinant Cell Line Bioreactor Cultivation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Bioreactor-batch and fed-batch cultures were performed only with the recombinant cell line at basal medium concentration. Experiments were performed in 1.6-L bioreactors (DASGIP, Julich, Germany) with 1.0-L working volume. The fed-batch protocol consisted of a temperature reduction (31 °C) on the seventh day and five intermittent feed additions (CD EfficientFeed™ B, Invitrogen, Carlsbad, CA, USA) of 10 % the initial volume starting on day 1. Cultures were seeded at a viable cell density of ~2 × 10⁵ cells/mL and maintained at 33 °C with an 80-rpm rate of agitation. Cultures were supplied with a gas mixture (2 sL/h) consisting of air, oxygen, nitrogen, and carbon dioxide to maintain a dissolved oxygen saturation of 40 %. All cultures were performed by duplicate with daily sampling. Batch cultures were terminated after 10 days and fed-batch cultures after 20 days. Samples for glucose, lactic acid, and antibody concentration determinations were collected and centrifuged at 5000 rpm for 5 min. The supernatant was stored at −20 °C for analysis.

López-Meza J., Araíz-Hernández D., Carrillo-Cocom L.M., López-Pacheco F., Rocha-Pizaña M.D, & Alvarez M.M. (2015). Using simple models to describe the kinetics of growth, glucose consumption, and monoclonal antibody formation in naive and infliximab producer CHO cells. Cytotechnology, 68(4), 1287-1300.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!