1,1'-Dioctadecyl-3,3,3',3'-tetramethylindotricarbocyanine iodide (DiR) was purchased from AAT Bioquest, Inc. (USA). DSPG, and soybean phosphatidylcholine S75 (S75) were obtained from Lipoid GmbH (USA). N (carbonyl-methoxy PEG 2000 )-1,2-distearoyl-sn-glycero-3-phosphoethanolamine (mPEG 2000 -DSPE) was obtained from Genzyme Corporation (USA). Medium-chain triglycerides (MCTs) were purchased from Beiya Medicated Oil Co., Ltd. (China). Bovine serum albumin (BSA) was supplied by Shanghai Seebio Biotech, Inc. (China). Horseradish peroxidase (HRP)-conjugated goat anti-rat IgM antibody was obtained from ICL Lab (USA). The compound 1,2-diaminobenzene was purchased from Sigma-Aldrich (USA). All the other reagents were of analytical grade and purchased commercially.
1 1 dioctadecyl 3 3 3 3 tetramethylindotricarbocyanine iodide dir
Manufactured by AAT Bioquest
Sourced in United States, China
1,1′-dioctadecyl-3,3,3′,3′-tetramethylindotricarbocyanine iodide (DiR) is a near-infrared fluorescent dye. It is a lipophilic carbocyanine dye that exhibits strong fluorescence in the near-infrared region of the spectrum.
Automatically generated - may contain errors
Lab products found in correlation
1 1 dioctadecyl 3 3 3 3 tetramethylindocarbocyanine perchlorate dii, by Beyotime (2 mentions)
Temozolomide tmz, by Meilun (2 mentions)
Penicillin, by Thermo Fisher Scientific (2 mentions)
Streptomycin, by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Chlorpromazine hydrochloride, by Merck Group (1 mentions)
Amiloride hydrochloride, by Merck Group (1 mentions)
Nonessential amino acid, by Thermo Fisher Scientific (1 mentions)
Genistein, by Merck Group (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Nocodazole, by Beyotime (1 mentions)
Monensin, by Beyotime (1 mentions)
Brefeldin a, by Beyotime (1 mentions)
Tween 80, by Maclin Biochemical Technology (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
Tetrahydrofuran, by Sinopharm (1 mentions)
Ascorbic acid, by Sinopharm (1 mentions)
4 6 diamino 2 phenylindole dapi, by Beyotime (1 mentions)
Dspe peg2000, by Laysan Bio (1 mentions)
L α phosphatidylcholine from egg yolk, by Merck Group (1 mentions)
13 protocols using 1 1 dioctadecyl 3 3 3 3 tetramethylindotricarbocyanine iodide dir
1
Synthesis and Characterization of DiR-Loaded Lipid Nanoparticles
2
Baicalein-Loaded Liposomes for Enhanced Cellular Uptake
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Baicalein (98% purity) was purchased from Nanjing Zelang Biological Technology Co. Ltd. Soybean phospholipids were purchased from Shanghai Tywei Pharmaceutical Co. Ltd. Ethylis oleas was purchased from Jiangxi Alpha High‐Tech Pharmaceutical Co., Ltd. Tween 80 was cgqzlied by Shanghai Macklin Biochemical Co., Ltd. Transcutol HP was kindly donated by Gattefossé. Ascorbic acid and tetrahydrofuran were cgqzlied by Sinopharm Chemical Reagent Co., Ltd. Minimum Essential Medium (MEM), fetal bovine serum, non‐essential amino‐acid, L‐glutamine, penicillin, streptomycin, and 0.25% trypsin were purchased from Thermo Fisher Scientific. Coumarin 6 (Cou‐6), methyl‐β‐cyclodextrin, chlorpromazine hydrochloride, genistein, and amiloride hydrochloride were purchased from Sigma‐Aldrich. Brefeldin A, monensin, nocodazole, and 4,6‐diamino‐2‐phenyl indole (DAPI) were purchased from Beyotime Biotechnology Co., Ltd. Bafilomycin A1 was cgqzlied by Meilunbio. 1,1′‐Dioctadecyl‐3,3,3′,3′‐tetramethylindotricarbocyanine iodide (DiR) was obtained from AAT Bioquest Inc. Tetramethylrhodaminyl (TRITC) phalloidin was purchased from Yeasen Biotechnology Co., Ltd.
3
Lipid Nanoparticle Formulation Protocols
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DSPE-PEG2000 was from Laysan Bio, Inc. (mPEG-DSPE, molecular weight 2000, Arab, AL, USA). L-α-Phosphatidylcholine (from egg yolk) was from Sigma-Aldrich (Catalog No. P-2772, St. Louis, MO, USA). F12511 and K604 were custom synthesized by WuXi AppTec in Shanghai, China. Based on the HPLC/MS and NMR profiles, F12511 was 98% in chemical purity and in stereospecificity (F12511 contains an asymmetric center) [51 (link)] and K604 was 98% in chemical purity. CP113,818 was a research gift from Pfizer. [1,1-dioctadecyl-3,3,3,3-tetramethylindotricarbocyanine iodide] DiR (catalog #22070) was purchased from AAT Bioquest, Pleasanton, CA, USA.
4
Fabrication of DiR-loaded PCL Nanoparticles
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FK506 was purchased from MedChem Express (Monmouth Junction, NJ, USA). Polycaprolactone (PCL, MW = 70 kDa-90 kDa) was obtained from the Qingdao Pansi Technology Co., Ltd. Acetone (ACE) and chloroform (DCM) were purchased from Sinopharm Group. 1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindotricarbocyanine iodide (DiR) was purchased from AAT Bioquest (California, USA). All other chemicals used were of analytical grade.
5
Synthesis and Characterization of Fluorescent Inositol Polyphosphates
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All lipids were purchased form Avanti Polar Lipids, Inc. (Alabaster, AL). Chloroform was obtained from Fisher Scientific. The 1,1′-dioctadecyl-3,3,3′,3′ tetramethylindotricarbocyanine iodide (DiR) was purchased from AAT Bioquest Inc. HEPES was purchased from Affymetrix, Inc. TiO2 beads were obtained from GL Sciences Inc. The 1,5-InsP8 and 1,5-PCP-InsP8 were synthesized as previously described.34 (link),46 The synthesis of 5-FAM-InsP7 began with a fluorenylmethyl derivative of InsP6 in which the only non-protected phosphate is at the 5-position.22 (link),48 The latter was phosphitylated with phosphoamidate and oxidized with meta-chloroperoxybenzoic acid following a strategy that proceeds via P(iii )–(v ) intermediates.46 ,47 Both reactions proceeded with a yield of 50% underlining the efficiency of the approach. Next, the alkyne modified product was used in an azide–alkyne Huisgen cycloaddition under copper catalysis resulting in the fluorescein modified FAM-5-InsP7 product with a yield of 93% (see ESI† ). The final preparation of 5-FAM-InsP7 was validated by PAGE62 (link) and by NMR (see ESI† ). The origin of the WT and PPIP5K KO HCT116 cells has been described previously.63 (link) The culture medium for these cells was DMEM/F12 plus 10% FBS (Germini Bio-Product) and 100 U mL–1 penicillin–streptomycin (ThermoFisher Scientific) at 37 °C with 5% CO2.
6
Aptamer-functionalized Theranostic Nanoparticles
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Polyethylene glycol (PEG), poly(lactic-co-glycolic acid) (PLGA) (50:50, MW=15000) and PLGA-PEG-COOH was purchased from Daigang BIO Engineer Ltd., Co. (Shan Dong, China). Chloroform (CHCl3) and isopropyl alcohol were purchased from Chongqing East Chemical Industry Ltd., Co. (Chongqing, China). Poly(vinyl alcohol) (PVA, MW=25000) were purchased from Sigma-Aldrich Chemical Co. (St Louis, MO, USA). Fluorescein isothiocyanate (FITC) were purchased from BioFroxx Ltd., Co. (Germany). 1,1-dioctadecyl-3,3,3,3-tetramethylindotricarbocyanine iodide (DiR) was purchased from AAT Bioquest Inc. (USA). 4,6-diamidino-2-phenylindole (DAPI) and 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI) were purchased from Beyotime Biotechnology Ltd., Co. (Shanghai, China). 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), N-hydroxysulfosuccinimide (sulfo-NHS), MES buffer and PFH were purchased from Aladdin Co., Ltd. (Shanghai, China). Aptamer CCFM641-529 (link) (sequence: 5ʹ-AGCAGCACAGAGGTCAGATGTGCGTGAGCGGTAGCCCCGTACGACCCACTGTGGTTGGGCCCTATGCGTGCTACCGTGAA-3ʹ, 80 bp), 5ʹ-NH2–modified CCFM641-5 aptamer and 5ʹ-FAM–modified CCFM641-5 aptamer were synthesized by Sangon Biotech Co., Ltd (Shanghai, China). Cell Counting Kit-8 (CCK-8) assay was purchased from Dojindo Laboratories (Kumamoto, Japan).
7
Formulation and Evaluation of Nanoparticles
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ACGs were obtained from Professor Jianyong Si’s laboratory (Institute of Medicinal Plant Development (IMPLAD), Beijing, China). Rh2 was purchased from Nanjing Spring & Autumn Biotech Co., Ltd. (Nanjing, China). Soybean phospholipids (SPCs) were provided by Shenyang Tianfeng Biological Pharmaceutical Co., Ltd. (Shenyang, China). Cholesterol was purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). 1,1-Dioctadecyl-3,3,3,3-tetramethylindotricarbocyanine iodide (DiR) was supplied by AAT Bioquest Inc. (Sunnyvale, CA). Temozolomide (TMZ) was purchased from Dalian Meilun Biological Technology Co., Ltd. (Dalian, China). All the other reagents were of analytical grade or higher. The water used was deionized in the research.
8
Synthesis and Characterization of Polymeric Nanoparticles
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Both DMC and bis-demethoxycurcumin, used as an internal standard (IS) for bioanalysis, were prepared by our laboratory and were tested for purity, which was >98.0%, using high-performance liquid chromatography (HPLC). Stannous octoate (Sn(Oct)2); ε-caprolactone (ε-CL); and monomethoxyl poly (ethylene glycol) (mPEG) with a number-average molecular weight (MN) of 2,000 were obtained from Sigma-Aldrich Co (St Louis, MO, USA). Pivaloyl chloride and 4-pyrrolidinopyridine were purchased from Wako Co (Osaka, Japan). N-t-butoxycarbonyl-phenylalanine (Boc-Phe) was provided by GL Biochem Co, Ltd (Shanghai, People’s Republic of China), and 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindotricarbocyanine iodide (DiR) was supplied by AAT Bioquest, Inc (Sunnyvale, CA, USA), respectively. Acetonitrile and methanol were both of HPLC grade and were purchased from Oceanpak Alexative Chemical (Gothenburg, Sweden). All other chemicals were reagent grade and were used as received. Purified water used throughout the study was provided by Wahaha Group Co, Ltd, (Hangzhou, People’s Republic of China).
9
Docetaxel-loaded Polymeric Nanoparticle Formulation
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Docetaxel (DTX) was purchased from HVSF United Chemical Materials Co., Ltd. (Beijing, People’s Republic of China). 1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindotricarbocyanine iodide (DiR) and coumarin 6 were purchased from AAT Bioquest (Sunnyvale, CA, USA) and Sigma-Aldrich Co. (St Louis, MO, USA). PEG2000–PDLLA2000 (polydispersity index [PDI] <1.8), PEG2000–PDLLA5000 (PDI <1.8), and PEG2000– PDLLA8000 (PDI <1.8) were purchased from Daigang Biomaterial Co., Ltd. (Jinan, People’s Republic of China). Water was purified by a Milli-Q water purification system (EMD Millipore, Billerica, MA, USA). All other chemicals and reagents were of analytical grade.
10
Multimodal Lipid-based Nanoparticle Synthesis
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Lipids, including 1,2-dipalmitoyl-sn-glycero-3-phosphocho-line (DPPC), 1,2-distearoyl-sn-glycero-3-phosphoethanola-mine-N-[amino(polyethyleneglycol)-2000] (DSPE-PEG2000-Amine), and DC-cholesterol hydrochloride (DC-CHOL) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). Chloroform (CHCl3) was purchased from Chongqing East Chemical Industry Ltd, Co. (Chongqing, China). Superparamagnetic iron oxide nanoparticles (Fe3O4, 10 nm, 25 mg/mL) were purchased from Ocean Nanotech Co. Ltd. (San Diego, USA). Perfluorohexanes (PFH, a PFC compound with a boiling point of 56°C) were purchased from Biofroxx Ltd, Co. (Shanghai, China). 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindotricarbocyanine iodide (DiR) was purchased from AAT Bioquest Inc. (USA). 2-(4-Amidinophenyl)- 6-indolecarbamidinedihyrochloride(DAPI), 1,1-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI) and fluorescein isothiocyanate (FITC) were purchased from Beyotime Biotechnology Co., Ltd (Shanghai, China).
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