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5 protocols using ethyl l lactate

1

Clay-Ceramic Composite Formation

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To prepare the materials of specified compositions, a 6 M NaOH solution (14 wt %) was used as a water and NaOH source for the clay green body formation promoting surface activation and vitrification. To facilitate sample compaction and compacted specimen extraction from the mould, the lubricant Zusoplast G 72 (Zschimmer & Schwarz Gmbh & Co KG, Lahnstein, Germany) was used at a level of 2.0 wt %. Furthermore, the surfactant (−)-Ethyl L-lactate, (cleaning grade, ≥98.0%, Sigma-Aldrich Chemie Gmbh, Munich, Germany) was used to improve clay and CS particle wetting [18 (link),24 (link),26 ] at a level of 2 wt %. Another 0.5 wt % of a dispersing agent on huminite-silicate basis, Dolaflux B 11 (Zschimmer & Schwarz Gmbh & Co KG, Lahnstein, Germany), was introduced. All the above weight percentage values reflect the dry clay-CS mixture as represented in Table 1, the clay/CS ratios also calculated in vol % for convenience. The specimens with 0, 10, 30, 50 and 60 vol % CS loading were designated as CE-0, CE-1, CE-3, CE-5 and CE-6, respectively.
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2

Comprehensive Analytical Standards for Wine Aroma Profiling

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Chromatographically pure standards included ethyl acetate, ethyl isobutyrate, isobutyl acetate, butyl acetate, ethyl 2-methylbutyrate, 2-methyl-1-propanol, isoamyl acetate, 1-butanol, 3-methyl-1-butanol, ethyl hexanoate, hexyl acetate, propanoic acid, ethyl L(-)-lactate, 1-hexanol, cis-3-hexenol, ethyl octanoate, 1-heptanol, 2,3-butanediol, 1-octanol, isobutyric acid, diethyl succinate, L-α-terpineol, phenethyl acetate, hexanoic acid, benzyl alcohol, phenylethyl alcohol, octanoic acid, decanoic acid, ethyl lactate, ethyl decanoate (Sigma-Aldrich, Shanghai, China). Ultrapure 18.2 MΩ cm water used for these experiments was produced from the Milli-Q Ultrapure water system (Human Corp., Seoul, Korea). Absolute ethanol (analytically pure) was purchased from Sichuan Xilong Chemical Industry Co. Ltd. (Chengdu, Sichuan, CHN).
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3

Larvae Injury Response Biochemical Modulation

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Chemical compounds were injected into larvae right after injury (Figure 4K-4M and 5K-5M), or right after the first imaging session (Figure 6I and S6E). Sodium L-lactate (Sigma Aldrich), ethyl L-lactate (Sigma Aldrich), disodium L-2-Hydroxyglutarate (Sigma Aldrich), octyl-L-2HG (Cayman Chemical), L-2-Hydroxybutyric acid (Sigma Aldrich), ethyl 2-Hydroxybutyrate (Fisher Scientific), L-tartaric acid (Sigma Aldrich) and diethyl L-tartrate (Sigma Aldrich) were used. Sodium L-lactate, disodium L-2-Hydroxyglutarate, L-2-Hydroxybutyric acid and L-tartaric acid were dissolved in water and pH was adjust to 7.2 before using. Final concentration of chemical compounds injected into larvae were calculated based on the volume estimated from larvae weight. Injection was performed by glass micropipettes and Hamilton syringes.
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Synthesis of Cyclic Polylactides

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l-Lactide, a product of PURAC was kindly supplied by Thyssen-Uhde (Berlin, Germany) and recrystallized from toluene (99.89% extra dry, ACROS Chemicals, Geel, Belgium). Ethyl l-lactate was purchased from Sigma-Aldrich (Taufkirchen, Germany) and used as received, tin(ii) 2-ethylhexanoate (>96% purity) was purchased from Alfa Aesar (Karlsruhe, Germany) and used as received. DSTL,24 (link) BuSnBuCa,25 (link) BuSnBiph26 (link) and SnBiph27 (link) were synthesized as described in previous publications dealing with syntheses of cyclic poly(l-lactide)s by means of these catalysts. Those publications also contain the experimental evidence for the cyclic topology of the resulting polylactides.
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5

Collagen-Functionalized PLA Microcarriers

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Poly(lactic acid) (Purasorb® PLDL 7038; 3.8 dL/g viscosity; Mw≈ 850,00 Da) was obtained from Corbion (Amsterdam, The Netherlands). (-)-Ethyl-L-lactate (purity > 99.0%), ammonium hydroxide (NH4OH), polyvinyl alcohol (PVA, 9–10 kDa, 80% hydrolyzed), N-hydroxysuccinimide 98% (NHS), Triton® X-100, Tris-EDTA 100X, and Sigmacote® were acquired from Sigma-Aldrich (Madrid, Spain). Cultispher® S gelatin microcarriers were kindly provided by Percell Biolytica (Astorp, Sweden). Nunc™ 96-Well Polystyrene Round Bottom Microwell Plates with non-treated surface were acquired from Thermo Fisher (Spain). Human recombinant collagen type I (hrCol I) was purchased from FibroGen (San Francisco, CA, USA). Sodium hydroxide (NaOH) was purchased from Panreac (Barcelona, Spain). 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide (EDAC), Glycine BioUltra, and Vectashield® Antifade Mounting Medium were acquired from Acros Organics (Amsterdam, The Netherlands), Fluka (Zaragoza, Spain), and Vectorlabs (CA, USA), respectively.
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