Labchip gx touch software

Manufactured by PerkinElmer

The LabChip GX Touch software is a data analysis tool designed for use with PerkinElmer's LabChip microfluidic platforms. The software provides automated analysis of sample data generated by the LabChip instruments.

Automatically generated - may contain errors

Lab products found in correlation

Labchip gx reviewer, by PerkinElmer (2 mentions) Sds polyacrylamide gel electrophoresis, by Bio-Rad (1 mentions) Melon gel igg purification kit, by Thermo Fisher Scientific (1 mentions) Nanodrop spectrophotometer, by Bio-Rad (1 mentions) Amicon ultra centrifugal filter, by Merck Group (1 mentions) Microtiter plate, by Bio-Rad (1 mentions) Labchip gxii touch ht, by PerkinElmer (1 mentions) Dithiothreitol (dtt), by Thermo Fisher Scientific (1 mentions) Iodoacetamide, by Merck Group (1 mentions)

2 protocols using labchip gx touch software

IgG Antibody Purification and Glycan Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

IgG antibodies were purified from plasma as described previously^{55 (link)}. In brief, total IgG was collected using the Melon Gel IgG Purification Kit (Thermo Fisher Scientific) according to the manufacturer’s protocol. Purified IgG samples were then centrifuged through 100-kDa Amicon Ultra Centrifugal Filters (Merck Millipore) at 14,000g for 15 min to remove excess serum proteins and buffer exchange antibodies into PBS. Purity was confirmed via SDS–polyacrylamide gel electrophoresis (Bio-Rad Laboratories) and IgG concentrations were measured using a NanoDrop spectrophotometer (Bio-Rad Laboratories). IgG N-linked glycosylation patterns were measured according to the ProfilerPro glycan profiling LabChip GXII Touch protocol on the LabChip GXII Touch HT Microchip-CE platform (PerkinElmer) using the LabChip GX Touch software (v.1.9.1010.0), as described previously^{55 (link)}. Microchip capillary electrophoresis laser-induced fluorescence analysis of digested and labeled N-linked glycans was performed. The relative prevalence of major N-linked glycan profiles of IgG was analyzed using the LabChip GX Reviewer (PerkinElmer) v.5.4.2222.0. Peaks were assigned based on the migration of known standards and glycan digests. The peak area and relative prevalence of each glycan pattern were calculated.

Zhang W., Kedzierski L., Chua B.Y., Mayo M., Lonzi C., Rigas V., Middleton B.F., McQuilten H.A., Rowntree L.C., Allen L.F., Purcell R.A., Tan H.X., Petersen J., Chaurasia P., Mordant F., Pogorelyy M.V., Minervina A.A., Crawford J.C., Perkins G.B., Zhang E., Gras S., Clemens E.B., Juno J.A., Audsley J., Khoury D.S., Holmes N.E., Thevarajan I., Subbarao K., Krammer F., Cheng A.C., Davenport M.P., Grubor-Bauk B., Coates P.T., Christensen B., Thomas P.G., Wheatley A.K., Kent S.J., Rossjohn J., Chung A.W., Boffa J., Miller A., Lynar S., Nelson J., Nguyen T.H., Davies J, & Kedzierska K. (2023). Robust and prototypical immune responses toward COVID-19 vaccine in First Nations peoples are impacted by comorbidities. Nature Immunology, 24(6), 966-978.

+ Open protocol

+ Expand

Protein Denaturation Analysis by LabChip GXII

Check if the same lab product or an alternative is used in the 5 most similar protocols

Analysis of all samples was performed on the LabChip GXII touch HT (PerkinElmer) according to the manufacturer’s protocol. Briefly, denatured protein was prepared by mixing 2 μl protein sample (1 mg/ml) with 14 μl of the denaturation solution (Protein Express Sample Buffer) provided by the manufacturer in the presence of 23 mM iodoacetamide (Sigma) for nonreducing condition or 34 mM DTT (Thermo Scientific) for reducing condition. The mixture was heated at 75 °C for 5 min and the samples run under both reducing and nonreducing conditions. The denatured protein was electrokinetically loaded directly into the chip from a microtiter plate (Bio-Rad Laboratories). The experiment was run using LabChip GX touch software (PerkinElmer) and data were analyzed using LabChip GX Reviewer (PerkinElmer).

Liu W., Maben Z., Wang C., Lindquist K.C., Li M., Rayannavar V., Lopez Armenta I., Nager A., Pascua E., Dominik P.K., Oyen D., Wang H., Roach R.C., Allan C.M., Mosyak L, & Chaparro-Riggers J. (2021). Structural delineation and phase-dependent activation of the costimulatory CD27:CD70 complex. The Journal of Biological Chemistry, 297(4), 101102.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!