Peg dithiol

Four-arm PEG–acrylate (10 kDa; 4-arm PEG–Ac) and PEG–dithiol (3.4 kDa; PEG–diSH) were obtained from Laysan Bio (Arab, AL, USA). PEG-diester-dithiol-1 (3.4 kDa; PEGDD-1) was synthesized in-house as described by us previously [26 (link)]. Fluoro-max red microspheres (2.0 µm in diameter, ex./em. 542 nm/612 nm) and human lys-plasmin native protein were obtained from ThermoFisher (Waltham, MA, USA). FluoroSpheresTM carboxylate-modified, infrared, 0.1 µm in diameter (ex./em. 715 nm/755 nm) were obtained from Invitrogen (Carlsbad, CA, USA). Bovine serum albumin (BSA), lysozyme, 1X phosphate buffer solution (PBS), 1X Dulbecco’s phosphate buffer solution (DPBS), triethanolamine (TEA), Bradford Protein Dye Reagent, and Triton X-100 were obtained from Sigma Aldrich (St. Louis, MO, USA). Olive oil was obtained from Costco (Issaquah, WA, USA). A 60-mL syringe and needles were obtained from Becton Dickinson (Franklin Lakes, NJ, USA) and 100 µL gas-tight syringe was obtained from Hamilton Company (Reno, NV, USA). Tubing and T-junctions for the microfluidic setup were obtained from Valco Instruments (Houston, TX, USA).