Carbachol

Manufactured by Fujifilm

Sourced in Japan

Carbachol is a chemical compound used in laboratory equipment. It functions as a cholinergic receptor agonist, activating muscarinic acetylcholine receptors.

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Lab products found in correlation

5 protocols using carbachol

Pharmacological Evaluation of 9-Phenanthrol

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9-Phenanthrol was purchased from Toronto Research Chemicals (North York, Canada).
Atropine, carbachol, and nystatin were purchased from Wako (Osaka, Japan). L-NAME
(Nω-Nitro-L-arginine methyl ester hydrochloride), guanethidine, α, β-methylene ATP,
collagenase type II and type XI, papain, dithiothreitol, and bovine serum albumin were
purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, U.S.A.). 9-Phenanthrol was
dissolved in dimethyl sulfoxide (DMSO), where the final concentration of DMSO up to 0.03%
had no effect on EFS- or carbachol-evoked contractions.

ALOM F., MATSUYAMA H., NAGANO H., FUJIKAWA S., TANAHASHI Y, & UNNO T. (2018). Involvement of transient receptor potential melastatin 4 channels in the resting membrane potential setting and cholinergic contractile responses in mouse detrusor and ileal smooth muscles. The Journal of Veterinary Medical Science, 81(2), 217-228.

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Calcium Signaling Modulation in Cell Experiments

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A-23107, BSA, KB-R7943 and carbachol were purchased from Wako Pure Chemical Industries (Osaka, Japan). 2-APB, amiloride, serotonin (5-HT), nifedipine, N-methyl-glucamine, noradrenaline, ouabain, U46619, 5-HT were purchased from Sigma-Aldrich (St. Louis, MO, U.S.A). Fluo-8 was purchased from Abcam (Cambridge, U.K.) Cytochalasin D was purchased from Cayman Chemical (Ann Arbor, MI, U.S.A.)

Ishida H., Yamaguchi M., Saito S.Y., Furukawa T., Shannonhouse J.L., Kim Y.S, & Ishikawa T. (2021). Na+-dependent inactivation of vascular Na+/Ca2+ exchanger responsible for reduced peripheral blood flow in neuropathic pain model. European journal of pharmacology, 910, 174448.

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Signaling Pathways Protocol

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ATP, apyrase, suramin, and epidermal growth factor (EGF) were purchased from Sigma‐Aldrich (St. Louis, MO). Capsaicin, 4α‐phorbol 12,13‐didecanoate (4α‐PDD) and carbachol were purchased from Wako Pure Chemical (Osaka, Japan). Trametinib was purchased from LC Laboratories (Woburn, MA). Pyridoxalphosphate‐6‐azophenyl‐2′,4′‐disulfonic acid (PPADS) was purchased from Abcam (Cambridge, MA). AG1478 was purchased from BioVision Inc. (Milpitas, CA).

Sano T., Kobayashi T., Negoro H., Sengiku A., Hiratsuka T., Kamioka Y., Liou L.S., Ogawa O, & Matsuda M. (2016). Intravital imaging of mouse urothelium reveals activation of extracellular signal‐regulated kinase by stretch‐induced intravesical release of ATP. Physiological Reports, 4(21), e13033.

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Krebs' Solution Preparation for Tissue Experiments

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During experiments, tissues were maintained in Krebs’ solution consisting of (mM): NaCl 118.4, KCl 4.7, CaCl₂ 2.5, MgSO₄ 1.2, KH₂PO₄ 1.2, NaHCO₃ 25 and glucose 11.7. ATP, tetraethylammonium chloride (TEA), tetrodotoxin, atropine sulfate monohydrate and carbachol were obtained from FUJIFILM-Wako (Osaka, Japan). D-tubocurarine, suramin, methoctramine hydrate, 4-diphenylacetoxy-N-methyl-piperidine methiodide (4-DAMP), pyridoxal phosphate-6-azophenyl-2,4-disulfonic acid (PPADS), cibacron blue F3GA (CBF3GA; synonym reactive blue 2) and 4-aminopyridine (4-AP) were obtained from Sigma-Aldrich (St Louis, MO, USA). Glibenclamide and nicorandil were obtained from Tokyo Chemical Industry (Tokyo, Japan). GSK1016790A was obtained from Cayman Chemical (Ann Arbor, MI, USA). Apamin was obtained from Peptide Institute (Osaka, Japan). tetrodotoxin was dissolved in citrate solution. Glibenclamide, nicorandil and GSK1016790A were dissolved in DMSO. Other drugs were dissolved in distilled water. The highest concentration of vehicles (0.1%) for the drugs alone had no effect on the basal tone and contractile responses at the concentrations used. The concentrations of drugs given were final concentrations in the bath solution.

Shiina T., Suzuki Y., Horii K., Sawamura T., Yuki N., Horii Y, & Shimizu Y. (2024). Purinergic inhibitory regulation of esophageal smooth muscle is mediated by P2Y receptors and ATP-dependent potassium channels in rats. The Journal of Physiological Sciences : JPS, 74, 26.

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Evaluation of Contractile Mechanisms

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ML204 was purchased from Focus Biomolecules (Plymouth Meeting, Pennsylvania, PA, U.S.A.), whereas atropine and carbachol were obtained from Wako (Osaka, Japan). L-NAME, guanethidine, and
α,β-methylene ATP were purchased from Sigma Chemical Co. (St. Louis, MO, U.S.A.). CPA was purchased from LKT Laboratories, Inc. (St. Paul, MN, U.S.A.) and 2-APB was purchased from StressMarq
Biosciences Inc. (Victoria, Canada). ML204 was dissolved in dimethyl sulfoxide (DMSO), where the final concentration of DMSO (up to 0.1%) had no effect on EFS- or carbachol-evoked
contractions.

ALOM F., MIYAKAWA M., MATSUYAMA H., NAGANO H., TANAHASHI Y, & UNNO T. (2018). Possible antagonistic effects of the TRPC4 channel blocker ML204 on M2 and M3 muscarinic receptors in mouse ileal and detrusor smooth muscles and atrial myocardium. The Journal of Veterinary Medical Science, 80(9), 1407-1415.

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