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Libra 120 system

Manufactured by Zeiss
Sourced in Japan, Germany

The Libra 120 system is a transmission electron microscope (TEM) designed and manufactured by Zeiss. The core function of the Libra 120 is to provide high-resolution imaging and analysis of materials at the nanoscale level. The system utilizes advanced electron optics and imaging capabilities to enable detailed examination of a wide range of samples.

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3 protocols using libra 120 system

1

Transmission Electron Microscopy Protocol

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TEM was conducted with a Hitachi H7650 system (Hitachi, Tokyo, Japan) at 80kV and a Zeiss Libra 120 system (Zeiss) at 120kV.
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2

Transmission Electron Microscopy Imaging

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For TEM, every formulation was prepared
in water, as described above. The undiluted sample (5 μL) was
deposited onto formvar-coated copper grids and dried overnight at
room temperature. Imaging was done on a Libra 120 system (Zeiss, Germany)
with a LaB6 filament and in column filter operating at
120 kV using a bottom mount camera Sharp:eye TRS (2 × 2 k) and
an in column camera Morada G2 (11 MP). Magnifications ranged from
8000- to 200,000-fold.
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3

Characterization of Coated AAV Nanoparticles

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AAV were coated with PAMAM conjugate as described above and processed in principle as described by Chen.[30] In brief, 2 µL sample was pipetted onto a formvar coated 300 mesh copper grid, left to evaporate for 6 h, then drop coated with 20 µL 1% phosphor tungstic acid solution for three minutes, excess liquid drained off and left to dry overnight. Samples were imaged on a Libra 120 system (Zeiss,) using a 120 kV operating filter and a LaB6 filament together with a Sharp:eye camera (TRS) and an in column camera Morada G2 (11 MP)
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