Paclitaxel oregon green 488 conjugate

Lipid stock solutions of DOPC and DOTAP in chloroform were purchased from Avanti Polar Lipids. PTXL was purchased from Acros Organics and dissolved in chloroform at 10.0 mM concentration. CellTiter 96® AQueous-One Solution Cell Proliferation Assay was obtained from Promega. Paclitaxel–Oregon Green® 488 Conjugate, Texas Red–DHPE, and glycerol monooleate (GMO) were purchased from Thermo Fisher Scientific as powders and dissolved in chloroform to 190 μM, 81 μM and 10 mM concentrations, respectively. Custom DNA oligomers (sense strand: ACGCTTT; antisense strand: AGCGTTT) were purchased from Sigma-Aldrich. Luciferase plasmid pGL3 (purchased from Promega) was propagated in Escherichia coli and purified using a Qiagen Plasmid Mega Prep Kit.

Lipid stock solutions of DOPC and DOTAP in chloroform were purchased from Avanti Polar Lipids. PTX was purchased from Acros Organics and dissolved in chloroform at 10.0 mM concentration. Paclitaxel-OregonGreen® 488 conjugate (PTX_GF) and TRITC-DHPE (lipid_RF) were purchased from Thermo Fisher Scientific as powders and dissolved in chloroform to 190 μM and 81 μM concentrations, respectively. 1-((3,4-dioleyloxybenzoyl)oxy)-ω -methoxy-poly(ethylene glycol) 2000 (PEG2K-lipid) was synthesized in a manner analogous to that described for ω -hydroxy PEG-lipids^{58 (link)} and dissolved in chloroform to 10 mM. CellTiter 96® AQueous-One Solution Cell Proliferation Assay was obtained from Promega.