Log In Sign up
The largest database of trusted experimental protocols

Lsm 880 nlo laser scanning confocal and multiphoton microscope

Manufactured by Zeiss
Visit Supplier

The LSM 880 NLO is a laser scanning confocal and multiphoton microscope developed by Zeiss. It combines the capabilities of confocal and multiphoton microscopy to enable high-resolution imaging of living and fixed samples. The system utilizes a tunable femtosecond pulsed laser for multiphoton excitation and an argon and helium-neon laser for confocal imaging.

Automatically generated - may contain errors

Lab products found in correlation

Prolong gold antifade with dapi, by Thermo Fisher Scientific (4 mentions) Fast green, by Merck Group (4 mentions) Tcs sp8 confocal laser scanning microscope, by Leica (2 mentions) Goat anti rabbit af488, by Thermo Fisher Scientific (2 mentions) Anti alexafluor 488 antibody, by Thermo Fisher Scientific (2 mentions) Inverted tcs sp8 confocal scanning laser microscope, by Leica (1 mentions)

Close spelling variants of this product

LSM 880 NLO laser scanning microscope LSM 880 confocal laser scanning microscope LSM 880 NLO confocal microscope LSM 880 multiphoton confocal microscope LSM 880 laser scanning microscope LSM 880 laser confocal microscope LSM 880 confocal scanning microscope Multiphoton confocal laser-scanning microscope Zeiss 880 laser scanning confocal microscope LSM confocal laser-scanning microscope

7 protocols using lsm 880 nlo laser scanning confocal and multiphoton microscope

1

Visualizing Nodose Ganglia Transcripts

Check if the same lab product or an alternative is used in the 5 most similar protocols
Nodose ganglia (NG) from C57Bl/6 mice were dissected as described above. Once removed ganglia were dipped in Fast Green (1%, Sigma-Aldrich) to assist with visualization when slicing and flash frozen in OCT. 15 um sections of NG were sliced on a cryostat for RNAScope. Samples were processed and stained with Scn5a, positive control or negative control probes according to the manufacturer’s instructions. Samples were mounted in Prolong gold antifade with DAPI (Thermo-Fisher) for imaging and imaged within 24 hours on an inverted LSM 880 NLO laser scanning confocal and multiphoton microscope (Zeiss) and images were processed using Image J.
Muller P.A., Schneeberger M., Matheis F., Wang P., Kerner Z., Ilanges A., Pellegrino K., del Mármol J., Castro T.B., Furuichi M., Perkins M., Han W., Rao A., Picard A.J., Cross J.R., Honda K., de Araujo I, & Mucida D. (2020). Microbes modulate sympathetic neurons via a gut-brain circuit. Nature, 583(7816), 441-446.
+ Open protocol
+ Expand
2

Multimodal Imaging of Intestinal Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole mount intestine, NG, DRG, and CG-SMG samples were imaged on an inverted LSM 880 NLO laser scanning confocal and multiphoton microscope (Zeiss) and on an inverted TCS SP8 laser scanning confocal microscope (Leica).
Muller P.A., Schneeberger M., Matheis F., Wang P., Kerner Z., Ilanges A., Pellegrino K., del Mármol J., Castro T.B., Furuichi M., Perkins M., Han W., Rao A., Picard A.J., Cross J.R., Honda K., de Araujo I, & Mucida D. (2020). Microbes modulate sympathetic neurons via a gut-brain circuit. Nature, 583(7816), 441-446.
+ Open protocol
+ Expand
3

Visualizing Colon-Innervating Neurons via CTB Labeling

Check if the same lab product or an alternative is used in the 5 most similar protocols
C57Bl/6 mice were injected bilaterally with CTB 488 into the colon as described above. NG were dissected 1 week post injection as described above, dipped in Fast Green (1%, Sigma-Aldrich) to assist with visualization and flash frozen in OCT. 15 um sections of NG were sliced on a cryostat for RNAScope/IHC. Samples were processed and stained with Scn5a, positive or negative control probes according to the manufacturer’s instructions. After in situ hybridization sections were washed three times in wash buffer (1X, ACDBio) and then fixed in 1% PFA in TBS for 10 minutes at 4 C to stabilize the ISH labeling. Samples were next washed three times in TBS-T and incubated in 10% Goat Serum in TBS with 1% BSA for 30 minutes. Samples were stained with anti Alexafluor-488 antibody (1:1000, Thermo-Fisher) for 1 hour in TBS-1% BSA. After primary antibody staining, sections were washed three times for 5 minutes each in TBST and stained with Goat anti rabbit AF488 (1:1000, Thermo-Fisher) in TBS-1% BSA for 30 minutes. Samples were again washed three times for 5 minutes each in TBST and finally mounted in Prolong gold antifade with DAPI (Thermo-Fisher) for imaging. Slides were imaged within 24 hours of mounting on an inverted LSM 880 NLO laser scanning confocal and multiphoton microscope (Zeiss) and images were processed using Image J.
Muller P.A., Schneeberger M., Matheis F., Wang P., Kerner Z., Ilanges A., Pellegrino K., del Mármol J., Castro T.B., Furuichi M., Perkins M., Han W., Rao A., Picard A.J., Cross J.R., Honda K., de Araujo I, & Mucida D. (2020). Microbes modulate sympathetic neurons via a gut-brain circuit. Nature, 583(7816), 441-446.
+ Open protocol
+ Expand
4

Visualizing Nodose Ganglia Transcripts

Check if the same lab product or an alternative is used in the 5 most similar protocols
Nodose ganglia (NG) from C57Bl/6 mice were dissected as described above. Once removed ganglia were dipped in Fast Green (1%, Sigma-Aldrich) to assist with visualization when slicing and flash frozen in OCT. 15 um sections of NG were sliced on a cryostat for RNAScope. Samples were processed and stained with Scn5a, positive control or negative control probes according to the manufacturer’s instructions. Samples were mounted in Prolong gold antifade with DAPI (Thermo-Fisher) for imaging and imaged within 24 hours on an inverted LSM 880 NLO laser scanning confocal and multiphoton microscope (Zeiss) and images were processed using Image J.
Muller P.A., Schneeberger M., Matheis F., Wang P., Kerner Z., Ilanges A., Pellegrino K., del Mármol J., Castro T.B., Furuichi M., Perkins M., Han W., Rao A., Picard A.J., Cross J.R., Honda K., de Araujo I, & Mucida D. (2020). Microbes modulate sympathetic neurons via a gut-brain circuit. Nature, 583(7816), 441-446.
+ Open protocol
+ Expand
5

Multimodal Imaging of Intestinal Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole mount intestine, NG, DRG, and CG-SMG samples were imaged on an inverted LSM 880 NLO laser scanning confocal and multiphoton microscope (Zeiss) and on an inverted TCS SP8 laser scanning confocal microscope (Leica).
Muller P.A., Schneeberger M., Matheis F., Wang P., Kerner Z., Ilanges A., Pellegrino K., del Mármol J., Castro T.B., Furuichi M., Perkins M., Han W., Rao A., Picard A.J., Cross J.R., Honda K., de Araujo I, & Mucida D. (2020). Microbes modulate sympathetic neurons via a gut-brain circuit. Nature, 583(7816), 441-446.
+ Open protocol
+ Expand
6

Visualizing Colon-Innervating Neurons via CTB Labeling

Check if the same lab product or an alternative is used in the 5 most similar protocols
C57Bl/6 mice were injected bilaterally with CTB 488 into the colon as described above. NG were dissected 1 week post injection as described above, dipped in Fast Green (1%, Sigma-Aldrich) to assist with visualization and flash frozen in OCT. 15 um sections of NG were sliced on a cryostat for RNAScope/IHC. Samples were processed and stained with Scn5a, positive or negative control probes according to the manufacturer’s instructions. After in situ hybridization sections were washed three times in wash buffer (1X, ACDBio) and then fixed in 1% PFA in TBS for 10 minutes at 4 C to stabilize the ISH labeling. Samples were next washed three times in TBS-T and incubated in 10% Goat Serum in TBS with 1% BSA for 30 minutes. Samples were stained with anti Alexafluor-488 antibody (1:1000, Thermo-Fisher) for 1 hour in TBS-1% BSA. After primary antibody staining, sections were washed three times for 5 minutes each in TBST and stained with Goat anti rabbit AF488 (1:1000, Thermo-Fisher) in TBS-1% BSA for 30 minutes. Samples were again washed three times for 5 minutes each in TBST and finally mounted in Prolong gold antifade with DAPI (Thermo-Fisher) for imaging. Slides were imaged within 24 hours of mounting on an inverted LSM 880 NLO laser scanning confocal and multiphoton microscope (Zeiss) and images were processed using Image J.
Muller P.A., Schneeberger M., Matheis F., Wang P., Kerner Z., Ilanges A., Pellegrino K., del Mármol J., Castro T.B., Furuichi M., Perkins M., Han W., Rao A., Picard A.J., Cross J.R., Honda K., de Araujo I, & Mucida D. (2020). Microbes modulate sympathetic neurons via a gut-brain circuit. Nature, 583(7816), 441-446.
+ Open protocol
+ Expand
7

Intestine Imaging Using Confocal Microscopy

Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole-mount intestine samples were imaged on an inverted LSM 880 NLO laser scanning confocal and multiphoton microscope (Zeiss) and on an inverted TCS SP8 laser scanning confocal microscope (Leica).
Matheis F., Muller P.A., Graves C.L., Gabanyi I., Kerner Z.J., Costa-Borges D., Ahrends T., Rosenstiel P, & Mucida D. (2020). Adrenergic signaling in muscularis macrophages limits infection-induced neuronal loss. Cell, 180(1), 64-78.e16.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!