Anemia was evaluated according to the diagnostic standards recommended by the World Health Organization through hematocrit (hemocytes and hemoglobin) indices with slight variations according to age and sex. The haemoglobin concentration was measured in venous blood using the Oiapoque Hospital's automated equipment (Mindray-BC-3000plus). Anemia was defined with haemoglobin reference values. The haematological parameters evaluated were the total number of erythrocytes (RBC; reference range: male 4.5–6.5 x 106/μL, female 3.9–5.6 x 106/μL and children aged 7–11 years 4.5–4.7 x 106/μL) and haemoglobin levels (Hb; males ≥ 13 g/dL, females ≥ 12 g/dLand children ≥ 11 g/dL). Individuals were considered anemic when their haemoglobin blood levels were ≤ 13 g/dL for males, ≤ 12 g/dL for females and ≥ 11 g/dL for the children.
Bc 3000 plus
Manufactured by Mindray
Sourced in China
The BC-3000 Plus is a hematology analyzer designed for clinical laboratory use. It is capable of performing complete blood count (CBC) analysis, including parameters such as red blood cell count, white blood cell count, and platelet count. The BC-3000 Plus utilizes advanced technology to provide accurate and reliable results for patient samples.
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Lab products found in correlation
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Thermomixer, by Eppendorf (1 mentions)
Selectra pro s, by Vital Scientific (1 mentions)
J2 21, by Beckman Coulter (1 mentions)
Hem 7211, by Omron (1 mentions)
Elisa test kit, by R&D Systems (1 mentions)
Isoflurane, by Abbott (1 mentions)
Bc 5150, by Mindray (1 mentions)
Selectra junior, by Vital Scientific (1 mentions)
19 protocols using bc 3000 plus
1
Anemia Diagnosis via Hematological Indices
2
Thrombocytopenia Evaluation in Pregnant Women
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Data on sociodemographic characteristics and clinical and other related factors were collected by trained data collectors. After the purpose of the study was explained, a laboratory technologist collected 4 mL of venous blood from each study participant into tubes containing ethylenediaminetetraacetic acid (EDTA) for complete blood count analysis and blood smear preparation. Platelet counts, platelet parameters, and other hematological parameters were measured using a MINDRAY BC-3000 PLUS automated hematology analyzer (Shenzhen Mindray Bio-Medical Electronics Co., Ltd., China). Thin blood smears were prepared from thrombocytopenic specimens, air-dried, labeled, and stained with Wright's stain to assess platelet morphology (small platelets and giant platelets) and the presence of malaria parasites. In addition, platelet aggregation was observed to distinguish between pseudo thrombocytopenia and true thrombocytopenia.
Thrombocytopenia is said to be present when the platelet count of the pregnant women is less than 150 × 109/L. The PLT count between 100 and 150 × 109/l is considered mild thrombocytopenia, levels ranging between 50 and 100 × 109/l are considered as moderate thrombocytopenia, and levels less than 50 × 109/L are considered as severe thrombocytopenia [22 (link)].
Thrombocytopenia is said to be present when the platelet count of the pregnant women is less than 150 × 109/L. The PLT count between 100 and 150 × 109/l is considered mild thrombocytopenia, levels ranging between 50 and 100 × 109/l are considered as moderate thrombocytopenia, and levels less than 50 × 109/L are considered as severe thrombocytopenia [22 (link)].
3
Nanofat Isolation and Nanoparticle Extraction
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Nanofat was obtained using a two-step centrifugation procedure consisting of collagenase I digestion, in accordance with the reported method (Tonnard et al., 2013 (link)). Briefly, after harvesting the fat tissue from the rats’ lower abdomen, the tissue was sliced into small pieces and digested with 0.2% collagenase I for 30 min at 37°C in the ThermoMixer (Eppendorf). After centrifugation at 210 × g for 5 min, cell pellets were obtained and resuspended in PBS, followed by filtration through a cell strainer (100 μm). The collected solution was washed using PBS thrice and concentrated to obtain Nanofat concentrates. The cell number in Nanofat was measured by a blood cell analyzer (Mindray BC-3000 plus, Shenzhen, China) and standardized to 1 × 106 cells/mL. Acquired Nanofat cells were treated with freeze–thawing (−80°C to 37°C) cycles thrice, followed by centrifugation at 2,000 × g for 5 min to remove residual fragments. The obtained supernatant was designated as NFL and stored at −80°C before use. Sketch map of NFL production was shown in Supplementary Figure S4 .
4
Incubator-Based Avian Embryo Preparation
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It was used during this study, an incubator (PAS REFORM®), electric sight, hatching trays, a centrifuge (HARAEUS Megafuge 1.0R), a hematology automat (MINDRAY BC 3000 Plus), and a biochemistry spectrophotometer (UV–1600 PC).
Five centiliter syringes and insulin syringes, precision balance, adhesive tape (Hypafix®), physiological water, hydrophilic cotton pads, EDTA, and dry tubes, pipettes, 100 uL and 1000 uL cones and others were used as consumables during this study.
Five centiliter syringes and insulin syringes, precision balance, adhesive tape (Hypafix®), physiological water, hydrophilic cotton pads, EDTA, and dry tubes, pipettes, 100 uL and 1000 uL cones and others were used as consumables during this study.
5
Fasting Blood Collection Protocol
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Participants were instructed not to exercise, not to change their diet, and not to take any medication or supplements for at least 48 h prior to blood sampling. Overnight-fasting venous blood samples were drawn between 08:30 a.m. and 10:00 a.m. The sample-collection day was selected according to the women's menstrual cycles.
Blood samples were allowed to clot at 25°C for 30 min and centrifuged at 2000 g for 10 min. Hematological analyses were performed with a hematology analyzer (BC-3000 Plus; Mindray, Shenzhen, China). Serum samples were stored at −82°C until analysis. The analyses were performed within 1 month.
Blood samples were allowed to clot at 25°C for 30 min and centrifuged at 2000 g for 10 min. Hematological analyses were performed with a hematology analyzer (BC-3000 Plus; Mindray, Shenzhen, China). Serum samples were stored at −82°C until analysis. The analyses were performed within 1 month.
6
Comprehensive Biomarker Profiling of Participants
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Prior to delivery, about 9 ml venous blood sample was collected from each participant into sodium citrate tubes for coagulation studies (Prothrombin Time - PT and activated Partial Thromboplastin Time - aPTT), serum separator tubes for biochemical assays (Total protein- TP, Albumin - ALB, Globulins-GLOB, Alanine Aminotransferase - ALT, Aspartate Aminotransferase - AST, urea -URE and creatinine - CRE) and ethylenediaminetetraacetic acid tubes (EDTA) tubes for haematological assays (Full Blood Count - FBC, Erythrocyte Sedimentation Rate - ESR, Glucose-6-phosphate Dehydrogenase - G6PD, sickling, blood group and peripheral blood smear).
Haematological assays were performed on fresh anticoagulated blood using an automated analyser (Mindray BC 3000 Plus, Shenzhen, China). Serum separator and sodium citrated samples were centrifuged and aliquots of plasma or serum were stored at −80°C until assayed. Biochemical assays were performed using an automated analyser (Selectra Pro S, Vital Scientific NV, Netherlands). Coagulation parameters were assayed using a semi-automated analyser (Coatron M4 TECO Medical Instruments, Germany). All other parameters were gathered from the patient records.
Haematological assays were performed on fresh anticoagulated blood using an automated analyser (Mindray BC 3000 Plus, Shenzhen, China). Serum separator and sodium citrated samples were centrifuged and aliquots of plasma or serum were stored at −80°C until assayed. Biochemical assays were performed using an automated analyser (Selectra Pro S, Vital Scientific NV, Netherlands). Coagulation parameters were assayed using a semi-automated analyser (Coatron M4 TECO Medical Instruments, Germany). All other parameters were gathered from the patient records.
7
Coenzyme Q10 and Precooling Effects on Blood
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Blood sampling was carried out 24 h before (stages 1 and 2) and after (stages 1 and 2) supplementation of CoQ 10 and precooling strategy (the highest level of effectiveness of CoQ 10 in blood) in four stages (17) . At both time points, approximately 5 ml of blood was sampled from an antecubital vein of right arms of the subjects by nursing expert before noon (from 08.00 to 11.00 hours). Then, 2 ml of it was poured and thoroughly stirred in test tubes with anticoagulant substances (K 2 EDTA) for blood cell count. The remaining blood (3 ml) was immediately transferred into gel tubes specific for serum without addition of an anticoagulant material for serum preparation and was centrifuged at 1500 g for 10 min at 4°C in a Beckman (J2-21) centrifuge (Beckman Coulter). Plasma samples were immediately frozen and stored at -80°C until analysis. Participants avoided any physical activity 24 h before blood sampling and recordings. Biochemical analysis of blood samples was conducted in equipped biochemistry laboratory of veterinary faculty (Department of Clinical Pathology, University of Urmia, Urmia, Iran). Also, laboratory equipment was evaluated 3 months before starting study. (25) with trivial modifications. Blood cell count was carried out using American Cell Counter of Mindray (BC-3000 plus).
8
High-altitude Physiological Assessment
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Data were collected by two physicians and an experienced sonographer who traveled between Yecheng and Ali. Measurement included height, weight, blood pressure (BP), heart rate (HR), arterial O2 saturation (SpO2), and blood samples. BP and HR were measured (HEM-7211; OMRON, Japan) in the supine position after 10 min of rest. SpO2 was measured using finger-pulse oximetry (YX303, Yuwell, Jiangsu, China) after finger warming and signal stabilization. Hemoglobin concentration (HB) and hematocrit (HCT) measurements (BC-3000Plus, Mindray, Shenzhen, China) were performed by peripheral venous sampling. From these data, body surface area (BSA) and body mass index (BMI) were calculated.
9
Comprehensive Hematological and Biochemical Analysis
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During slaughter, 25 blood samples were collected from each variety in heparinized tubes. The hematological parameters were determined by using Automatic Fully Digital Hematology Analyzer (BC-3000 Plus, Shenzhen Mindray, Bio-Medical Electronics Co., LTD, Shenzhen, China). These parameters were total count of red blood cells (RBC), hemoglobin (HGB), hematocrit (HT), and thrombocytes. The collected blood samples were centrifuged at 4000× rpm for 15 min. The resulting plasma samples were frozen at −20 °C for further analysis. The plasma concentrations of total protein, albumen, total cholesterol, and triglycerides were spectrophotometrically determined according to Fathi et al. [10 (link)] using commercial reagent kits (Stanbio Laboratory, Boerne, TX, USA). The globulin was calculated as the difference between the total protein and albumen.
10
Parasite Identification and Hematological Analysis
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Parasite identification was performed by microscopy of stained smears of scrapings of lesion borders and by isolation in culture (17 ). Briefly, smears of scrapings of lesion borders in triplicate were stained with the Panotic fast system and microscopically assessed for amastigote forms of the parasite. The isolation by culture was performed by inoculation of aspirates into tubes containing blood agar base No2 and 10% rabbit defibrinated blood (18 ). The tubes were incubated at 26°C for 10 days and the promastigote form of parasite was microscopically assessed. Total number of white blood cells, neutrophils, eosinophils and lymphocytes (as fraction of white blood cells), red blood cells, hemoglobin, and hematocrit were measured within 30 min after blood sampling using the Auto hematology analyzer BC-3000 Plus, Mindray™ (Nanshan, Shenzhen, China) at the Villa Tunari Hospital. ASAT, ALAT, urea, and glucose in serum were also measured by photometry. The transferrin receptor was measured by an ELISA test kit (R&D Systems) at the IIBISMED laboratories. The other clinical chemistry measurements were performed at the Clinical Chemistry Laboratory of Skåne University Hospital, Lund, Sweden, using certified methods. These data were compared with the reference ranges for healthy subjects established at that laboratory.
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