Anti gr 1 pe clone rb6 8c5

The spleen and thymus of the mice were removed under aseptic conditions and then ground through a 75-μm filter. Mice thymocytes were cultured in RPMI medium containing 1 μg/mL Con A or 1 μg/mL Con A + 100 U/mL IL-2 to stimulate cell proliferation. The cells were inoculated into a culture medium in a 96-well plate, a CCK-8 kit (Kesaien) was added, and a microplate reader (Molecular Devices) was used to detect the OD450 nm. Mice spleen cells were first stained with CFSE (10 μM), and then cultured in RPMI medium containing 1 μg/mL ConA or 1 μg/mL ConA + 100 U/mL IL-2. The cells were removed and incubated with anti-CD3ε-PE (clone 145-2C11, BioLegend), anti-CD19-PE-cy7 (clone 6D5, BioLegend), and anti-Gr-1-PE (clone RB6-8C5, BioLegend) antibodies in PBS for 30 min at 4 °C. Finally, flow cytometry (Beckman) was performed to analyze the T, B, and granulocytes and detect the average fluorescence intensity using the FITC channel.