Biomek fxp automated workstation

Manufactured by Beckman Coulter

The Biomek FXP Automated Workstation is a liquid handling system designed for a wide range of laboratory applications. It features precise liquid handling capabilities, customizable deck configurations, and integrated robotic arms for automated sample processing. The Biomek FXP provides reliable and reproducible results to support various scientific workflows.

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Lab products found in correlation

Qubit 2.0 fluorometer, by Thermo Fisher Scientific (2 mentions) Tapestation 4200, by Agilent Technologies (1 mentions) Nextseq 550 system, by Illumina (1 mentions) Bluepippin size selection, by Sage Science (1 mentions) Spectramax quant dsdna assay kit, by Molecular Devices (1 mentions) Gemini xps fluorometer, by Molecular Devices (1 mentions) Smrtbell template preparation kit, by Pacific Biosciences (1 mentions) D1000 screentape, by Agilent Technologies (1 mentions) G tube fragmentation, by Covaris (1 mentions) Prism 8, by GraphPad (1 mentions) Celltiter glo luminescent cell viability assay, by PerkinElmer (1 mentions) Multiflo fx multi mode dispenser, by Agilent Technologies (1 mentions) Infinite m200 plate reader, by Tecan (1 mentions) Cells to ct kit, by Thermo Fisher Scientific (1 mentions) Freedom evo 200, by Tecan (1 mentions) Cyquant cell proliferation assay kit, by Thermo Fisher Scientific (1 mentions) Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions) Lumitrac 200, by Greiner (1 mentions)

5 protocols using biomek fxp automated workstation

Bacterial DNA Isolation and Sequencing

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DNA was extracted from bacterial isolates using the Agencourt GenFind DNA Isolation Kit and the Biomek FX^P Automated Workstation (Beckman Coulter). A short-read NGS library was prepared using the Nextera DNA Flex or XT Library Prep Kit (Illumina, San Diego, CA, USA). DNA quantification was performed in a microplate using the SpectraMax Quant dsDNA Assay Kit and the Gemini XPS Fluorometer (Molecular Devices, San Jose, CA, USA), or in a single tube using the Qubit 2.0 fluorometer (Thermo Fisher Scientific, Springfield Township, NJ, USA). Library quality was examined using the 4200 TapeStation and D1000 ScreenTape (Agilent, Santa Clara, CA, USA). Paired-end sequencing (2 × 150 cycles) was performed using the NextSeq 550 system (Illumina). The Pacific Biosciences (PacBio, Menlo Park, CA, USA) RSII single-molecule real-time (SMRT) sequencing system was employed for the long-read sequencing of A. baumannii PB364. The long-read NGS library was processed using g-TUBE fragmentation (Covaris, Woburn, MA, USA), BluePippin size selection (Sage Science, Beverly, MA, USA) and a SMRTbell template preparation kit (PacBio). Mainly, the manufacturers’ standard protocols were followed.

Huang W., Wang G., Yin C., Chen D., Dhand A., Chanza M., Dimitrova N, & Fallon J.T. (2019). Optimizing a Whole-Genome Sequencing Data Processing Pipeline for Precision Surveillance of Health Care-Associated Infections. Microorganisms, 7(10), 388.

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Cell Viability Assay Protocol

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Proliferating cells were seeded at approximately 20 × 103 cells per 50 μL per well into 96 well plates using a MultiFlo FX multimode dispenser (BioTek). On reaching ~40% cell density, the cells were then incubated with varying concentrations (195 nM to 200 μM final) of test samples using a Biomek FXP Automated Workstation (Beckman Coulter). 48 hours post drug incubation, cell viability was determined using Promega’s CellTiter-Glo Luminescent Cell Viability Assay according to the manufacturer’s protocol on a plate reader (EnVision from PerkinElmer) in Luminescence mode. In each experiment, each unique condition (i.e., different sample type and concentration) was tested in triplicate. The percentage of test sample induced toxicity was calculated with respect to DMSO treated cells, and graphed to give dose response curves. IC50 values for each treatment were calculated using GraphPad Prism 8.0 Software.

Ali R., Guan Y., Leveille A.N., Vaughn E., Parelkar S., Thompson P.R, & Mattson A.E. (2019). Synthesis and Anticancer Activity of Structure Simplified Naturally-Inspired Dimeric Chromenone Derivatives. European journal of organic chemistry, 2019(41), 6917-6929.

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Luminescence-based Biosensor Assay

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Plasma was diluted 12.5-fold into a 96-well plate, and, subsequently, 10 μl was dispensed into an individual flat-bottom white plate (Greiner Lumitrac 200, 384-well plates) using a Biomek FX^P Automated Workstation (Beckmann Coulter). Next, 10 μl of 1 nM biosensors was dispensed using Thermo Multidrop Combi Reagent Dispenser (Thermo Fisher Scientific) to assay plates and incubated at room temperature for 20 min. Then, 15 μl of substrate was added using the same reagent dispenser and incubated at room temperature for 10 min. Luminescence was read on a Tecan M200 infinite plate reader with an integration time of 1,000 ms.

Elledge S.K., Zhou X.X., Byrnes J.R., Martinko A.J., Lui I., Pance K., Lim S.A., Glasgow J.E., Glasgow A.A., Turcios K., Iyer N.S., Torres L., Peluso M.J., Henrich T.J., Wang T.T., Tato C.M., Leung K.K., Greenhouse B, & Wells J.A. (2021). Engineering luminescent biosensors for point-of-care SARS-CoV-2 antibody detection. Nature biotechnology, 39(8), 928-935.

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High-throughput esiRNA Screening of Cell Proliferation

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The esiRNA screening was performed on Tecan Freedom EVO 200 Automated workstation (Tecan Group Ltd., Switzerland, RRID:SCR_016771), equipped with RoMa (Robotic Manipulator Arm) and LiHa (Liquid Handling Arm) with 1 ml dilutors. Briefly, ESCs were reversely transfected with esiRNAs and Lipofectamine RNAiMAX (Invitrogen 56532) in 96-well plates. Subsequently, cells were treated with APH or ATRi 36 hr post-transfection. Twelve hours after treatment, cell proliferation was assessed by CyQUANT Cell Proliferation Assay kit (ThermoFisher C35011), followed by cell lysis using Cells-to-CT Kit (ThermoFisher, AM1728). The Taqman qPCR assays were performed using Biomek FXP Automated Workstation (Beckman Coulter Life Sciences).

Atashpaz S., Samadi Shams S., Gonzalez J.M., Sebestyén E., Arghavanifard N., Gnocchi A., Albers E., Minardi S., Faga G., Soffientini P., Allievi E., Cancila V., Bachi A., Fernández-Capetillo Ó., Tripodo C., Ferrari F., López-Contreras A.J, & Costanzo V. (2020). ATR expands embryonic stem cell fate potential in response to replication stress. eLife, 9, e54756.

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High-Throughput Serum Screening Protocol

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To facilitate high-throughput screening of serum, a semi-automated approach was developed and simulated using the UCSF Antibiome Center robotics platform⁴⁰. Serum in 96-well plates is first diluted 12.5-fold, and 10 μl is dispensed into four individual flat-bottom white plates (Greiner Lumitrac 200, 384-well plates) using a Biomek FX^P Automated Workstation (Beckmann Coulter). Serum-containing assay plates are then transferred to a robotics protocol with dispensing of biosensor and substrate followed by luminescence reading. Although one iteration of 96 samples takes 40 min, each additional iteration takes an additional 3.5 min, limited by luminescence reading (1 s per well plus plate transfer). As such, it is estimated that 40 plates (3,840 assays) could be run in 3 h. The robotics run was developed and simulated using Thermo Momentum software (v5.0.6).

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