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Ultrospec 3000pro

Manufactured by GE Healthcare
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Sourced in Sweden

The Ultrospec 3000pro is a UV-Vis spectrophotometer designed for laboratory use. It is capable of measuring the absorbance of samples across a range of wavelengths. The device features a high-quality optical system and advanced electronics to provide accurate and reliable results.

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Lab products found in correlation

Zetapals, by Brookhaven Instruments (3 mentions)

4 protocols using ultrospec 3000pro

1

Nanoparticle Characterization: Size, Charge

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Hydrodynamic diameter and surface charge of nanoparticles and aggregates were characterized by dynamic light scattering (DLS) and zeta potential measurements on the ZetaPALS instrument (Brookhaven Instruments Corporation). For zeta measurements, 40 μL of nanoparticle solution was diluted to 1.2 mL with 1 mM KNO3 immediately prior to measurement. UV absorption spectra were taken on the Ultrospec 3000pro (GE Lifesciences). Aggregates were visualized by transmission electron microscopy (TEM). 4 μL of aggregates solution was dried onto a formvar-stabilized 200 or 300 mesh copper–carbon grid purchased from Ted Pella. TEM images were taken using FEI Tecnai 12 Twin transmission electron microscope.
Zhang Y., Liu A.T., Cornejo Y.R., Van Haute D, & Berlin J.M. (2020). A Systematic comparison of in vitro cell uptake and in vivo biodistribution for three classes of gold nanoparticles with saturated PEG coatings. PLoS ONE, 15(7), e0234916.
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2

Comprehensive Particle Characterization

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Hydrodynamic diameter (HD) and polydispersity (PDI) of particles were characterized by dynamic light scattering (DLS) on the ZetaPALS instrument (Brookhaven) and the number-weighted average size of particles was measured by Nanosight (Malvern Instruments). Surface charge was characterized by zeta potential measurements on the ZetaPALS instrument (Brookhaven). UV-vis absorption spectra were measured on the Ultrospec 3000pro (GE Lifesciences). All particles were visualized by transmission electron microscopy (TEM).
Zhang Y., Abidi W, & Berlin J.M. (2019). Colloidal Capsules Assembled from Gold Nanoparticles using Small Molecule Hydrophobic Crosslinkers. Langmuir : the ACS journal of surfaces and colloids, 35(52), 17037-17045.
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3

Nanoparticle Characterization Protocol

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To determine particle concentration and hydrodynamic diameter, particles were diluted 100 times in DiH2O, mixed, and injected into the Nanosight (Malvern Instruments). To determine zeta potential, 10 μL of particles were diluted into 1.4mL of DiH2O and analyzed using ZetaPals (Brookhaven Instruments). UV absorbance was measured on Ultrospec 3000pro (GE Life Sciences). In order to visualize the aggregates, 2 μL of aggregates solution were dried onto a formvar stabilized 200 mesh copper carbon grid purchased from TED Pella. TEM images were taken using FEI Tecnai 12 Twin.
Van Haute D., Liu A.T, & Berlin J.M. (2018). Coating Metal Nanoparticle Surfaces With Small Organic Molecules Can Reduce Non-Specific Cell Uptake. ACS nano, 12(1), 117-127.
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4

Clavulanic Acid Production Quantification

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The fermented broth was centrifuged at 5500 x g for 20 min at 4 °C, and the cell pellet was washed twice with distilled water and dried to constant weight at 80 °C. Clavulanic acid concentration in the fermented broth was determined spectrophotometric ally by its reaction with imidazole (Bird et al., 1982 ). For this purpose, the increase in the optical density at 311 nm consequent to the formation of the product [1-(8-hydroxy-6-oxo-4-azooct-2-enol)-imidazole] was determined using a UV/Vis spectrophotometer, model Ultrospec 3000 pro (GE Healthcare, Life Sciences, Uppsala, Sweden). Glycerol concentration was determined according to Hae Bok and Demain (1977) (link).
Viana Marques D.A., Santos-Ebinuma V.D., de Oliveira P.M., Lima G.M., Araújo J.M., Lima-Filho J.L., Converti A., Pessoa-Júnior A, & Porto A.L. (2014). Screening of wild type Streptomyces isolates able to overproduce clavulanic acid. Brazilian Journal of Microbiology, 45(3), 919-928.
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