6120 quadrupole lc ms

Manufactured by Agilent Technologies

Sourced in United States

The 6120 Quadrupole LC/MS is a liquid chromatography-mass spectrometry (LC/MS) system designed for qualitative and quantitative analysis of chemical compounds. It features a quadrupole mass analyzer for detection and identification of a wide range of molecules in complex samples.

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17 protocols using 6120 quadrupole lc ms

Characterization of Synthesized Compounds

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The uncorrected melting points of the synthesized compounds were measured using a Gallenkamp melting point device. A Perkin Elmer FT-IR Spectrum BX device was used to record the infrared (IR) spectra (as KBr disks). The nuclear magnetic resonance (NMR) spectra of the synthesized compounds were measured after dissolving the test samples in DMSO-d₆ and he measurements were achieved at 500 MHz for ₁H and 125.76 MHz for ₁₃C on Bruker NMR spectrometer at the Research Center, College of Pharmacy, King Saud University, Saudi Arabia. Chemical shifts are articulated in δ-values (ppm) compared to tetramethylsilane as an internal standard. Elemental analyses of the target compounds were executed at the Microanalysis Laboratory, Cairo University, Cairo, Egypt, and the results agreed favorably with the proposed structure within ±0.4% of the theoretical values (Table S1). Agilent Quadrupole 6120 LC/MS was utilized to record the mass spectra of the synthesized compounds with the aid of electrospray ionization (ESI) source. Silica gel thin layer chromatography plates with fluorescent indicator at 254 nm were acquired from Merck and visualization was accomplished by illumination with a UV light source (254 nm).

Al-Wabli R.I., Al-Ghamdi A.R., Ghabbour H.A., Al-Agamy M.H, & Attia M.I. (2019). Synthesis, structure elucidation, and antifungal potential of certain new benzodioxole–imidazole molecular hybrids bearing ester functionalities. Drug Design, Development and Therapy, 13, 775-789.

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Time-Dependent Degradation of TLQP-21 Peptide

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TLQP-21 was incubated in 100% mouse plasma (100uL) at concentration at 1mM at 37°C for the duration of the study. At the time points of 0 min, 10 min, 30 min, and 60 min, aliquots of the incubated solutions were withdrawn (15uL) and diluted with 3x acetonitrile/ethanol mixture (v/v=l:l, 45uL) to precipitate the plasma proteins, and peptides were present in solution. The mixture was subsequently treated by microcentrifugation at 15,000 rpm for 2 min to remove the precipitation, the upper clear supernatant was collected and subjected to the LC-MS (Agilent Quadrupole 6120 LC-MS, ESI mode) analysis using a linear gradient of buffer B over buffer A (A: 100% water, 0.05% TFA; B: 90% aqueous acetonitrile, 0.05% TFA) on a Kinetex C8 column (2.6μΜ, 100Å, 75×4.6mm). The area under the curve of the peak at each time point was compared to the 0 min time point for calculating the percentage of the remaining peptide, and the degraded peptide products were studied by analyzing the Mass data of newly formed peaks.

Guo Z., Sahu B., He R., Finan B., Cero C., Verardi R., Razzoli M., Veglia G., Di Marchi R.D., Miles J.M, & Bartolomucci A. (2018). Clearance kinetics of the VGF-derived Neuropeptide TLQP-21. Neuropeptides, 71, 97-103.

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Synthesis of Benzyl Cinnamate Derivatives

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All commercial regents: 2,4-dihydroxyacetophenone (99%), benzaldehyde (99%), cinnamic acid, benzyl bromide, dicyclohexylcarbodiimide (99%) dimethyl sulfoxide, potassium carbonate, potassium hydroxide, dichloromethane anhydrous (99%), methanol and boron tribromide were obtained from Sigma–Aldrich (St. Louis, MO, USA) and were used without further purification. Melting points were determined in a Prendo and were uncorrected. Nuclear magnetic resonance spectra were determined at 600 MHz for ¹H NMR and 150 MHz for ¹³C NMR in the presence of tetramethylsilane (TMS) as an internal standard in CDCl₃ on a Bruker AMX 600 instrument. Chemical shifts δ are expressed in parts per million (ppm) relative to TMS and coupling constants (J) in Hertz. Multiplicities are indicated as singlet (s), doublet (d), triplet (t), quartet (q), double of double (dd), multiplet (m), and broad singlet (bs). Open column chromatographies were carried out on silica gel 60 (70–230 mesh), and different solvent systems of n-hexane and EtOAc were used as mobile phases for the purification. Mass spectra were obtained in a Jeol M-station JEOL JMX-AX 505 HA mass spectrometer and HPLC Agilent Infinity 1260 coupled Quadrupole LC/MS 6120.

Ventura-Salazar I.A., Palacios-Can F.J., González-Maya L., Sánchez-Carranza J.N., Antunez-Mojica M., Razo-Hernández R.S, & Alvarez L. (2023). Finding a Novel Chalcone–Cinnamic Acid Chimeric Compound with Antiproliferative Activity against MCF-7 Cell Line Using a Free-Wilson Type Approach. Molecules, 28(14), 5486.

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Quantification of Isoflavones and Anthocyanins

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The concentrations of isoflavones and anthocyanins were analyzed using reversed phase high-pressure liquid chromatography (RP-HPLC) with electrospray ionization mass spectrometry (ESI-MS) detection. Extraction and chromatographic analysis procedures were based on previously published methods, with certain modifications (Zhang et al., 2011 (link); Liu et al., 2016 (link)). Twelve common isoflavones and eight anthocyanins were quantified by external standardization using an Agilent 1260-series high performance liquid chromatography (HPLC) system equipped with a mass spectrometric detector (Agilent Quadrupole LC/MS 6120). The isoflavones and anthocyanins were identified by comparing the sample retention times and mass spectra with those of standard compounds, and absolute quantification was carried out via linear regression of corresponding standards.

Deng J.C., Yang C.Q., Zhang J., Zhang Q., Yang F., Yang W.Y, & Liu J. (2017). Organ-Specific Differential NMR-Based Metabonomic Analysis of Soybean [Glycine max (L.) Merr.] Fruit Reveals the Metabolic Shifts and Potential Protection Mechanisms Involved in Field Mold Infection. Frontiers in Plant Science, 8, 508.

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HPLC-MS Analysis of Organic Compound

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The mobile phase comprised methanol/water (70 : 30, v/v), with isocratic elution at the flow rate of 1.0 mL min⁻¹ and a column temperature of 30 °C. The detection wavelength was set at 254 nm, and the injection volume was 10 μL.
The identification of the converted product was performed via mass spectrometry (ESI-MS) with electrospray ionization (ESI)/quadrupole (Q) ESI-MS data (quadrupole LC/MS 6120, Agilent Technologies, Palo Alto, CA) in the direct injection mode, and the MS conditions were as follows: mass range: 100–1000 m/z, capillary temperature: 300 °C, spray voltage: 4 kV, skimmer voltage: 40 V, auxiliary gas (nitrogen) flow: 6 L min⁻¹, and positive mode. NMR spectra were obtained using the Bruker AV-400 spectrometer (Bruker BioSpin, Rheinstetten, Germany) with TMS as the internal standard.

Wang D., Khan M.S., Cui L., Song X., Zhu H., Ma T., Li X, & Sun R. (2019). A novel method for the highly efficient biotransformation of genistein from genistin using a high-speed counter-current chromatography bioreactor. RSC Advances, 9(9), 4892-4899.

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Fmoc-based Solid-phase Peptide Synthesis

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Synthesis of both peptides was performed on a 0.022 mmol scale. Peptides were synthesized using standard Fmoc-based solid-phase synthesis with HBTU. Fmoc was deprotected with 20% piperidine in dimethylformamide. The resin was cleaved with 95% trifluoroacetic acid, 2.5% triisopropylsilane and 2.5% dichloromethane (1 mL) for one hour. After ether precipitation, peptides were purified using reverse-phase HPLC. Final pure peptides were identified (≥95% purity) using LC/MS (Agilent Technologies 6120 Quadrupole LC/MS).

Cassell R.J., Mores K.L., Zerfas B.L., Mahmoud A.H., Lill M.A., Trader D.J, & van Rijn R.M. (2018). Rubiscolins are naturally occurring G protein-biased delta opioid receptor peptides. European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology, 29(3), 450-456.

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Extraction and Quantification of Stilbenes from Berry Skins

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Extraction of total stilbenes from berry skins was conducted as described by Vrhovsek et al. [51 (link)]. The quantification of piceid and resveratrol was performed by injecting 10 μl of extract in an Agilent 1100 Series HPLC coupled with an Agilent Technologies 6120 Quadrupole LC/MS. Reverse phase separation was carried out in a column Synergi 4u Fusion-RP 80, 150 × 4.6 mm 4 μm Phenomenex, flow rate was 0.5 mL/min using gradient Solvent A (1% formic acid in water) and solvent B (1% formic acid in acetonitrile). The solvent gradient was as follows: 0–20 min from 90 to 40% solvent A; 20–21 min from 40 to 10% solvent A; 21–26 min hold 10% solvent A; 26–27 min from 10 to 90% solvent A; 27–37 min hold 90% solvent A. The compounds were determined and quantified by comparing with trans-resveratrol (R5010 Sigma-Aldrich, St. Louis) and piceid (15,721 Sigma-Aldrich, St. Louis) standards.

Ferreira V., Matus J.T., Pinto-Carnide O., Carrasco D., Arroyo-García R, & Castro I. (2019). Genetic analysis of a white-to-red berry skin color reversion and its transcriptomic and metabolic consequences in grapevine (Vitis vinifera cv. ‘Moscatel Galego’). BMC Genomics, 20, 952.

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Organic Synthesis Characterization Methods

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The solvents and reagents were purchased from Bidepharm (Shanghai, China) or Aladdin (Shanghai, China) without further purification unless otherwise indicated. The column chromatography was performed on silica gel (200–300 mesh). ¹H and ¹³C NMR spectra were recorded in DMSO-d₆ using a Bruker AC-300/400P spectrometer, and TMS as a standard. Chemical shifts (δ values) and coupling constants (J values) are measured in ppm and Hz, respectively. ESI mass spectra were performed using an Agilent Technologies 6120 quadrupole LC-MS.

Bao J., Hao Y., Ni T., Wang R., Liu J., Chi X., Wang T., Yu S., Jin Y., Yan L., Li X., Zhang D, & Xie F. (2023). Design, synthesis and in vitro biological studies of novel triazoles with potent and broad-spectrum antifungal activity. Journal of Enzyme Inhibition and Medicinal Chemistry, 38(1), 2244696.

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Peptide Synthesis and Purification

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All amino acids and resins were purchased from Novabiochem (Merck Millipore) and Carbosynth. All other synthetic reagents were obtained from Novabiochem, Sigma-Aldrich, or Carl Roth. Peptide synthesis was performed on a MultiPep RSi peptide synthesizer (Intavis Bioanalytical Instruments AG). Peptides were purified using a 1260 Infinity II preparative HPLC system (Agilent Technologies) with a VP 250/10 NUCLEODUR 100-5 C18 ec column (Macherey-Nagel) running a general gradient of 10–70% acetonitrile (ACN) in H₂O and validated using a 1260 Infinity I HPLC System coupled to a 6120 Quadrupole LC-MS (Agilent Technologies) with an EC 250/4 NUCLEODUR 100-5 C18 ec column (Macherey-Nagel) with a general gradient of 10–90% ACN in H₂O, and additionally a Microflex LT MALDI (Bruker) was used for the analysis.

Schwarz J.J., Grundmann L., Kokot T., Kläsener K., Fotteler S., Medgyesi D., Köhn M., Reth M, & Warscheid B. (2021). Quantitative proteomics identifies PTP1B as modulator of B cell antigen receptor signaling. Life Science Alliance, 4(11), e202101084.

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Peptide Synthesis and Purification

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All amino acids and resins were purchased from Novabiochem (Merck, Darmstadt, Germany). All other synthetic reagents were obtained from Novabiochem, Sigma-Aldrich (St. Louis, Missouri, USA), or Carl Roth (Karlsruhe, Germany). Peptide synthesis was performed on a MultiPep RSi peptide synthesizer (Intavis Bioanalytical Instruments AG, Köln, Germany). Peptides were purified using a 1260 Infinity II Prep HPLC system (Agilent Technologies, Santa Clara, California, USA) with a VP 250/10 NUCLEODUR 100-5 C18 ec column (Macherey-Nagel, Düren Germany) running a general gradient of 10% to 50% Acetonitrile (ACN) in H₂O and validated using a 1260 Infinity I HPLC System coupled to a 6120 Quadrupole LC/MS (Agilent Technologies, Santa Clara, California, USA) with an EC 250/4 NUCLEODUR 100-5 C18 ec column (Macherey-Nagel) also running a general gradient of 10% to 50% ACN in H₂O and a Microflex LT MALDI (Bruker, Bremen, Germany).

Hoermann B., Kokot T., Helm D., Heinzlmeir S., Chojnacki J.E., Schubert T., Ludwig C., Berteotti A., Kurzawa N., Kuster B., Savitski M.M, & Köhn M. (2020). Dissecting the sequence determinants for dephosphorylation by the catalytic subunits of phosphatases PP1 and PP2A. Nature Communications, 11, 3583.

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