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2 protocols using apc rat anti mouse cd8a 53 6.7

1

Murine Immune Cell Phenotyping

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The following antibodies/kits were bought from BD Biosciences (USA): PerCP-Cy-5.5 Rat Anti-Mouse CD4-(RM4-5) (550954), APC-Rat-Anti-Mouse-CD8a-(53-6.7) (561093), PE-Rat-Anti-Mouse-CD45R/B220-(RA3-6B2) (553089) and mouse Cytometric Bead Array-(CBA) kit. The reagents Anti-Human/Mouse-Rorγt-PE-(AFKJS-9) (12-6988), Anti-Mouse/Rat-Foxp3-APC-(FJK-16s) (17-5773), Foxp3/Transcription factor staining buffer (0-5523-00), RBC lysis buffer (00-4300-54) were obtained from eBioscience (USA). Anti-Mouse-CD254-(TRANCE-RANKL) PE (IK22/5) (510005), Anti-Mouse-CD8-PE/Cy7 (53-6.7) (100721), Anti-Mouse-IFN-γ-FITC (XMG 1.2) (505805), Anti-Mouse-IL-10-FITC (JESS-16E3) (505005), Anti-Mouse-IL-17A-PE (TC11-8H10.1) (506903) were obtained from BioLegend (USA).
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2

Multiplex Flow Cytometric Analysis

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The following antibodies/kits were bought from BD Biosciences (USA): PerCP-Cy-5.5 Rat Anti-Mouse CD4-(RM4-5) (550954), APC-Rat-Anti-Mouse-CD8a-(53-6.7) (561093), PE-Rat-Anti-Mouse-CD45R/B220-(RA3-6B2) (553089) and mouse Cytometric Bead Array-(CBA) kit. The reagents Anti-Human/Mouse Rorγt-PE-(AFKJS-9) (12-6988), Anti-Mouse/Rat Foxp3-APC-(FJK-16s) (17-5773), Foxp3/Transcription factor staining buffer (0-5523-00), RBC lysis buffer (00-4300-54) were obtained from eBioscience (USA). Anti-Mouse-CD254-(TRANCE-RANKL)-PE (IK22/5) (510005), Anti-Mouse-CD8-PE/Cy7 (53-6.7) (100721), Anti-Mouse-IFN-γ-FITC (XMG 1.2) (505805) and Anti-Mouse-IL-17A-PE (TC11-8H10.1) (506903) were obtained from BioLegend (USA). Mouse/Rat Estradiol ELISA Kit (Calbiotech, Spring Valley, CA, USA) and all procedures were followed according to the manufacturer's instruction.
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