Bu6 4

Manufactured by GeneTex

Bu6-4 is a laboratory equipment product offered by GeneTex. It is designed to provide a specific function within the laboratory setting. However, a detailed and unbiased description of its core function cannot be provided while maintaining the requested level of conciseness and objectivity. Additional information may be available from the manufacturer or other reliable sources.

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Lab products found in correlation

Bmc9318, by Roche (2 mentions) Dylight 649 anti mouse, by Jackson ImmunoResearch (1 mentions) Alexa fluor 488 anti rabbit, by Jackson ImmunoResearch (1 mentions) Rabbit anti fibrillarin antibody, by Abcam (1 mentions) B44 and 3d4, by BD (1 mentions) Chicken polyclonal anti brdu antibody, by Abcam (1 mentions) Mouse anti sc35 antibody, by Abcam (1 mentions) Rabbit anti h1.2 antibody, by Abcam (1 mentions) Mouse anti coilin antibody, by Abcam (1 mentions) Dylight 649 anti chicken antibodies, by Jackson ImmunoResearch (1 mentions) Bu20a and mobu 1, by Exbio (1 mentions) Bu20a, by BioLegend (1 mentions) Bu 33, by Merck Group (1 mentions) Alexa fluor 488, by Jackson ImmunoResearch (1 mentions)

2 protocols using bu6 4

Multimodal Imaging of DNA Synthesis

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The following primary antibodies were used: mouse anti-BrdU antibody clones: B44 and 3D4 (BD Biosciences), Bu20a and MoBu-1 (Exbio Praha), BMC9318 (Roche), Bu5.1 (Millipore), Bu6-4 (GeneTex), B33 (Sigma Aldrich) and chicken polyclonal anti-BrdU antibody (Abcam). For the concurrent localisation of DNA synthesis and cellular proteins, we used following antibodies: mouse anti-SC35 antibody (Abcam), rabbit anti-H1.2 antibody (Abcam), rabbit anti-PCNA antibody (proliferating cell nuclear antigen; Abcam), mouse anti-coilin antibody (Abcam), mouse anti-MBD4 antibody (Santa Cruz Biotechnology), rabbit anti-fibrillarin antibody (Abcam) and mouse anti-mitochondrial antibody (MTC02; Abcam).
The following secondary antibodies were used: DyLight 649 anti-mouse, Alexa Fluor^® 488 anti-mouse, Alexa Fluor^® 488 anti-rabbit and DyLight 649 anti-chicken antibodies (all Jackson ImmunoResearch).

Ligasová A., Konečný P., Frydrych I, & Koberna K. (2017). Looking for ugly ducklings: The role of the stability of BrdU-antibody complex and the improved method of the detection of DNA replication. PLoS ONE, 12(3), e0174893.

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Comparative Evaluation of Anti-BrdU Antibodies

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The following monoclonal anti-bromodeoxyuridine antibody clones were tested in this study: BMC9318 (Roche), Bu-33 (Sigma Aldrich), B44 (Becton Dickinson), Bu6-4 (Genetex), Bu20a (BioLegend) and Bu5.1 (Millipore). As a secondary antibody, we used anti-mouse antibody conjugated with the fluorochrome Alexa Fluor 488 (1:100, Jackson ImmunoResearch).

Ligasová A., Liboska R., Rosenberg I, & Koberna K. (2015). The Fingerprint of Anti-Bromodeoxyuridine Antibodies and Its Use for the Assessment of Their Affinity to 5-Bromo-2'-Deoxyuridine in Cellular DNA under Various Conditions. PLoS ONE, 10(7), e0132393.

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