The following mouse monoclonal antibodies were used: v-Src (#327, Calbiochem), Src (GD11, Millipore), phosphotyrosine (pTyr, 4G10, Upstate Biotechnology), and actin (C4, Millipore). The following rabbit monoclonal antibodies were used: phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204) (D13.14.4E, Cell Signaling Technology), and p21 Waf1/Cip1 (12D1, Cell Signaling Technology). The following rabbit polyclonal antibodies were used: c-Src (N-16, Santa Cruz Biotechnology), ERK2 (C-14, Santa Cruz Biotechnology), Src[pY416] (phospho-Src family, Cell Signaling Technology), p16 (C-20, Santa Cruz Biotechnology), p21 (C-19, Santa Cruz Biotechnology) and Cleaved Caspase-3 (Asp175, Cell Signaling Technology). The following rat monoclonal antibody was used: α-tubulin (MCA78G, Serotec). Horseradish peroxidase-F(ab’)2 fragments of anti-mouse IgG antibody (GE Healthcare), anti-rabbit IgG antibody (Cell LAB), and anti-rat IgG antibody (GE Healthcare) were used. Alexa Fluor 488-donkey-anti-mouse IgG and Alexa Fluor 647-goat-anti-mouse IgG secondary antibodies were obtained from Invitrogen.
α tubulin mca78g
Manufactured by Bio-Rad
α-tubulin (MCA78G) is a monoclonal antibody that recognizes the α-tubulin protein. α-tubulin is a key component of the cytoskeleton and is involved in various cellular processes, including cell division and intracellular transport.
Automatically generated - may contain errors
Lab products found in correlation
P21 waf1 cip1 12d1, by Cell Signaling Technology (1 mentions)
Alexa fluor 488 donkey anti mouse igg, by Thermo Fisher Scientific (1 mentions)
Cleaved caspase 3 asp175, by Cell Signaling Technology (1 mentions)
Alexa fluor 647 goat anti mouse igg, by Thermo Fisher Scientific (1 mentions)
Erk2 c 14, by Santa Cruz Biotechnology (1 mentions)
Actin c4, by Merck Group (1 mentions)
Phospho p44 42 mapk erk1 2 thr202 tyr204 d13.14.4e, by Cell Signaling Technology (1 mentions)
Src py416 phospho src family, by Cell Signaling Technology (1 mentions)
Anti rat igg antibody, by GE Healthcare (1 mentions)
Anti src py416, by Cell Signaling Technology (1 mentions)
Cyclin b1 h 433, by Santa Cruz Biotechnology (1 mentions)
Anti rabbit igg antibody, by GE Healthcare (1 mentions)
2 protocols using α tubulin mca78g
1
Antibody-Based Protein Expression Analysis
2
Antibody Panel for Cell Cycle Analysis
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The following antibodies are used: Lyn (clone 9, Wako; H-6, sc-7274, Santa Cruz Biotechnology), actin (C4, Millipore; 13E5, Cell Signaling Technology), phosphotyrosine (pTyr) (4G10, Upstate Biotechnology Inc.), Src (#327, Calbiochem), anti-Src[pY416] (phospho-Src family, Cell Signaling Technology), cyclin B1 (H-433, Santa Cruz Biotechnology; V152, Cell Signaling Technology), phospho-histone H3 serine at position 10 (H3pS10) (clone 6G3, #9706 S, Cell Signaling Technology), and α-tubulin (MCA78G, Serotec). Horseradish peroxidase (HRP)-F(ab′)2 fragments of anti-mouse IgG antibody, anti-rabbit IgG antibody, and of anti-rat IgG antibody, and Alexa Fluor 488-donkey-anti-mouse IgG antibody were purchased from GE Healthcare, Cell LAB, and Invitrogen.
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