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Mouse monoclonal anti transferrin receptor antibody

Manufactured by Thermo Fisher Scientific
Sourced in United States

The mouse monoclonal anti-transferrin receptor antibody is a laboratory reagent used to detect and quantify the transferrin receptor protein in various biological samples.

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2 protocols using mouse monoclonal anti transferrin receptor antibody

1

Immunoblot Analysis of L1 Phosphorylation

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Horseradish peroxidase-conjugated cholera toxin B subunits were obtained from Sigma-Aldrich (St Louis, MO, USA). Mouse monoclonal anti-transferrin receptor antibody was from Invitrogen (Carlsbad, CA, USA). Goat polyclonal anti-neural cell adhesion molecule L1 cytoplasmic domain (anti-L1CD) was from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The mouse monoclonal antibody 74-5H7 that recognizes dephosphorylated Y1176 on the cytoplasmic tail of L1 was previously described (Schaefer et al., 2002). Monoclonal antibody to phosphotyrosine (PY-100) was obtained from Cell Signaling (Danvers, MA, USA). Polyclonal antibody to beta actin was obtained from ThermoFisher Scientific (Waltham, MA, USA). All secondary antibodies for immunoblot analysis were from Jackson Immuno Research Laboratories (West Grove, PA, USA).
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2

Antibodies for Insulin Signaling Pathway Analysis

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Rabbit, polyclonal anti-Akt rabbit polyclonal anti-phospho-Akt (Ser 473), rabbit polyclonal anti-phospho-AS160 (Ser 588), rabbit polyclonal anti-phosho-AS160 (Thr 642) AS160, rabbit polyclonal anti-FoxO1(Ser 256 Fox) were from Cell Signaling Technology, Inc. (Danvers, MA). Goat polyclonal anti-GLUT4 (C-20) (used for western blot), rabbit polyclonal anti-insulin Rb (C-19) rabbit polyclonal anti-IRAP, rabbit polyclonal anti-IRS-1(A-19), mouse monoclonal anti-phosphotyrosine (PY20) were from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Mouse monoclonal anti-GAPDH, rabbit polyclonal anti-GLUT4 (used for immuno-fluorescence) antibody were from Abcam (Cambridge, MA). Mouse monoclonal anti-transferrin receptor antibody was from Invitrogen (Madison, WI). Mouse monoclonal anti-GM130 was from BD Transduction Laboratories (Mississauga, ON). Mouse monoclonal anti-Flag antibody was from Sigma-Aldrich (St. Louis, MO).
Anti-rabbit and anti-mouse antibodies conjugated with HRP were from Pierce/Thermo Fisher Scientific Inc. (Rockford, IL), anti-goat conjugated with HRP antibody was from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Anti-rabbit Alexa 488 or Alexa 594 and anti-mouse Alexa 488 or Alexa 594 were from Invitrogen (Madison, WI).
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