Beas2B cells expressing mTagBFP2::EML4-ALK and GFP::DCP1B were seeded into Nunc Lab-Tek 8-well imaging chambers. A custom-made sample holder ensured the imaging chamber fits securely on the microscope stage without position shift. The cells were first imaged in regular RPMI cell culture media and then the media was replaced by RPMI media containing 5% hexanediol (Sigma). The same field of view was monitored at defined time points after addition of hexanediol.
Hexanediol
Manufactured by Merck Group
Sourced in Sweden
Hexanediol is a clear, colorless liquid that is commonly used as a chemical building block in the production of various products. It has the chemical formula C6H14O2. Hexanediol is primarily employed as a precursor in the synthesis of other chemical compounds, but its specific core function is not provided in this factual and unbiased description.
Automatically generated - may contain errors
Lab products found in correlation
Lab tek 8 well imaging chambers, by Thermo Fisher Scientific (1 mentions)
Acetic acid anhydride, by Merck Group (1 mentions)
Methanol, by Thermo Fisher Scientific (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Acetic acid, by Thermo Fisher Scientific (1 mentions)
Chloroform, by Thermo Fisher Scientific (1 mentions)
Toluene, by Merck Group (1 mentions)
Dichloromethane, by Merck Group (1 mentions)
Dichloromethane dcm, by Thermo Fisher Scientific (1 mentions)
Diethyl carbonate, by Merck Group (1 mentions)
1 8 diazabicyclo 5.4.0 undec 7 ene, by Merck Group (1 mentions)
Trimethylolpropane allyl ether, by Merck Group (1 mentions)
Sodium hydride nah, by Merck Group (1 mentions)
1 4 butanediol, by Merck Group (1 mentions)
Deuterated chloroform, by Merck Group (1 mentions)
Diphenyl ether, by Merck Group (1 mentions)
Diethyl sebacate, by Merck Group (1 mentions)
Diethyl succinate, by Merck Group (1 mentions)
Diethyl adipate, by Merck Group (1 mentions)
Dimethyl 2 5 furandicarboxylate, by Fluorochem (1 mentions)
4 protocols using hexanediol
1
Visualizing Stress Granule Dynamics
2
Synthesis of Polymeric Precursors
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Hexanediol (Sigma-Aldrich, Sweden) and 1,8-diazabicyclo[5.4.0]undec-7-ene
(DBU) (Sigma-Aldrich, Sweden) were stored under an inert gas atmosphere
prior to use. Chloroform (HPLC grade, Fisher Scientific, Germany),
methanol (general purpose grade, Fisher Scientific, Germany), dichloromethane
(anhydrous, ≥ 99.8%, Sigma-Aldrich), toluene (anhydrous, ≥
99.8%, Sigma-Aldrich), acetonitrile (anhydrous, ≥ 99.8%, Sigma-Aldrich),
dichloromethane (DCM) (Fisher Scientific, Germany), sodium hydride
(NaH) (60% dispersion in mineral oil, Sigma-Aldrich, Sweden), diethyl
carbonate (99%, Sigma-Aldrich, Sweden), trimethylolpropane allyl ether
(98%, Sigma-Aldrich, Sweden), acetic acid (technical grade, Fisher
Scientific, Germany), and acetic acid anhydride (ReagentPlus, ≥
99%, Sigma-Aldrich, Sweden) were used as received.
(DBU) (Sigma-Aldrich, Sweden) were stored under an inert gas atmosphere
prior to use. Chloroform (HPLC grade, Fisher Scientific, Germany),
methanol (general purpose grade, Fisher Scientific, Germany), dichloromethane
(anhydrous, ≥ 99.8%, Sigma-Aldrich), toluene (anhydrous, ≥
99.8%, Sigma-Aldrich), acetonitrile (anhydrous, ≥ 99.8%, Sigma-Aldrich),
dichloromethane (DCM) (Fisher Scientific, Germany), sodium hydride
(NaH) (60% dispersion in mineral oil, Sigma-Aldrich, Sweden), diethyl
carbonate (99%, Sigma-Aldrich, Sweden), trimethylolpropane allyl ether
(98%, Sigma-Aldrich, Sweden), acetic acid (technical grade, Fisher
Scientific, Germany), and acetic acid anhydride (ReagentPlus, ≥
99%, Sigma-Aldrich, Sweden) were used as received.
3
Enzymatic Synthesis of Diesters and Diols
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Novozym 435 (N435, Candida antarctica lipase B (CALB) immobilized on acrylic resin, ≥5000 U g−1), 1,4‐butanediol (1,4‐BDO, 99 %), 1,6‐hexanediol (1,6‐HDO, 99 %), 1,8‐octanediol (1,8‐ODO, 98 %), 1,10‐decanediol (1,10‐DDO, 98 %), 1,12‐dodecanediol (1,12‐DODO, 99 %), diethyl succinate (99 %), diethyl adipate (99 %), diethyl sebacate (≥98 %), deuterated chloroform (CDCl3, 99.8 atom% D), chloroform (CHCl3, Chromasolv HPLC, ≥99.8 %, amylene stabilized) and diphenyl ether (99 %) were purchased from Sigma–Aldrich. Dimethyl 2,5‐furandicarboxylate (DMFDCA, 97 %) was purchased from Fluorochem UK. Diethyl dodecanedioate (≥95 %) was purchased from TCI Europe. Diethyl suberate (99 %) was purchased from ABCR. 2,5‐Bis(hydroxymethyl)furan (BHMF, ≥98 %) was purchased from Apollo Scientific. Chloroform (CHCl3, ChromAR HPLC, ethanol stabilized) and n‐hexane (n‐Hx, 99 %) were obtained from Macron. Absolute methanol (MeOH, AR) was obtained from Biosolve Chemicals. N435 was predried as reported previously.5b diphenyl ether was distilled at 140 °C under reduced pressure and stored with activated 4 Å molecular sieves before use. All other chemicals were used as received.
4
Spermatogenic Cell Inhibitor Rescue Assay
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10-µM stock solutions of nocodazole and paclitaxel (Sigma-Aldrich), along with 5 mM stock of MLN 8237 Aurora A Kinase inhibitor (Cayman Chemical, Ann Arbor, MI) were prepared in DMSO and stored at − 80 °C. All drug stocks were diluted to final concentrations in mEKRB. Rescue experiments from inhibitors for isolated spermatogenic cells were performed by centrifuging cells at 125×g for 3 min, discarding supernatant (inhibitor), and adding back 2 ml of inhibitor-free mEKRB to cell pellets for 15–60 min recovery at 34 °C. Cold treatment was performed in ice-cold water at 4 °C in mEKRB for 15 min, with rescue experiments performed by a 15-min recovery at 34 °C. Hexanediol (Sigma-Aldrich) was suspended in mEKRB at a final concentration of 3.5% just before use47 (link).
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